C6orf134 Antibody - #DF13487

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Product: C6orf134 Antibody
Catalog: DF13487
Description: Rabbit polyclonal antibody to C6orf134
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 46kDa; 47kD(Calculated).
Uniprot: Q5SQI0
RRID: AB_2846506

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
Clonality:
Polyclonal
Specificity:
C6orf134 Antibody detects endogenous levels of total C6orf134.
RRID:
AB_2846506
Cite Format: Affinity Biosciences Cat# DF13487, RRID:AB_2846506.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Acetyltransferase mec 17 homolog; Acetyltransferase mec-17 homolog; Alpha TAT; Alpha tubulin acetyltransferase 1; Alpha tubulin N acetyltransferase; Alpha-TAT; Alpha-tubulin N-acetyltransferase; ATAT_HUMAN; ATAT1; Chromosome 6 open reading frame 134; Hypothetical protein LOC79969; MEC17; Nbla00487; TAT;

Immunogens

Immunogen:
Uniprot:

Q5SQI0(ATAT_HUMAN) >>Visit HPA database.

Gene(ID):
ATAT1(79969)
Sequence:
MEFPFDVDALFPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIIDELGKASAKAQNLSAPITSASRMQSNRHVVYILKDSSARPAGKGAIIGFIKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHRPPAPSLRATRHSRAAAVDPTPAAPARKLPPKRAEGDIKPYSSSDREFLKVAVEPPWPLNRAPRRATPPAHPPPRSSSLGNSPERGPLRPFVPEQELLRSLRLCPPHPTARLLLAADPGGSPAQRRRTRGTPPGLVAQSCCYSRHGGVNSSSPNTGNQDSKQGEQETKNRSASEEQALSQDGSGEKPMHTAPPQAPAPPAQSWTVGGDILNARFIRNLQERRSTRPW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Rabbit
88
Bovine
50
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5SQI0 As Substrate

Site PTM Type Enzyme
K56 Methylation
K56 Ubiquitination
Y142 Phosphorylation
K162 Ubiquitination
K165 Ubiquitination
K233 Acetylation
S237 Phosphorylation
T261 Phosphorylation
S270 Phosphorylation
S272 Phosphorylation
S276 Phosphorylation
R293 Methylation
S315 Phosphorylation
T322 Phosphorylation
R323 Methylation
S333 Phosphorylation
K380 Acetylation

Research Backgrounds

Function:

Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.

PTMs:

Autoacetylation strongly increases tubulin acetylation.

Subcellular Location:

Cytoplasm. Membrane>Clathrin-coated pit. Cell junction>Focal adhesion. Cell projection>Axon. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Spindle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the BBSome complex. Interacts with AP2 alpha-adaptins, including AP2A2, but not with AP1 gamma-adaptin (AP1G1/AP1G2); this interaction is required for efficient alpha-tubulin acetylation, hence clathrin-coated pits are sites of microtubule acetylation.

Family&Domains:

Belongs to the acetyltransferase ATAT1 family.

References

1). Inhibition of miR-221-3p promotes axonal regeneration and repair of primary sensory neurons via regulating p27 expression. NeuroReport, 2021 (PubMed: 37161985) [IF=1.7]

Application: WB    Species: Rat    Sample:

Fig. 4 (a) Immunofluorescence micrographs of DRG neurons in the five groups. α-Tubulin staining of DRG neurons is shown in red and DAPI counterstaining in blue (400×). (b) Expression of p27 and α-tubulin relative fluorescence intensity in the five groups. (c) Relative protein expression levels of p27 and GAP-43 were normalized to actin in the five groups. The data were presented as mean ± SD of triplicate experiments (n = 3; *P 

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