TCF21 Antibody - #DF13477

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Product: TCF21 Antibody
Catalog: DF13477
Description: Rabbit polyclonal antibody to TCF21
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 19kDa, 37 kDa; 20kD(Calculated).
Uniprot: O43680
RRID: AB_2846496

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(86%), Bovine(100%), Horse(86%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(86%)
Clonality:
Polyclonal
Specificity:
TCF21 Antibody detects endogenous levels of total TCF21.
RRID:
AB_2846496
Cite Format: Affinity Biosciences Cat# DF13477, RRID:AB_2846496.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bHLHa23; Capsulin; Class A basic helix-loop-helix protein 23; Epicardin; POD 1; Pod-1; POD1; Podocyte expressed 1; Podocyte-expressed 1; TCF-21; tcf21; TCF21_HUMAN; Transcription factor 21;

Immunogens

Immunogen:
Uniprot:

O43680(TCF21_HUMAN) >>Visit HPA database.

Gene(ID):
TCF21(6943)
Sequence:
MSTGSLSDVEDLQEVEMLECDGLKMDSNKEFVTSNESTEESSNCENGSPQKGRGGLGKRRKAPTKKSPLSGVSQEGKQVQRNAANARERARMRVLSKAFSRLKTTLPWVPPDTKLSKLDTLRLASSYIAHLRQILANDKYENGYIHPVNLTWPFMVAGKPESDLKEVVTASRLCGTTAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Pig
86
Horse
86
Xenopus
86
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43680 As Substrate

Site PTM Type Enzyme
K24 Sumoylation
S67 Phosphorylation
Y140 Phosphorylation

Research Backgrounds

Function:

Involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis. May play a role in the specification or differentiation of one or more subsets of epicardial cell types.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Efficient DNA binding requires dimerization with another bHLH protein. Forms a heterodimer with TCF3 and binds the E box (5'-CANNTG-3').

References

1). Characterizing cellular heterogeneity in fibrotic hypersensitivity pneumonitis by single-cell transcriptional analysis. Cell Death Discovery, 2022 (PubMed: 35091537) [IF=7.0]

Application: IF/ICC    Species: Human    Sample: lung tissues

Fig. 4: Single-Cell transcriptional analysis unravels heterogeneity of fibroblasts during FHP.A t-SNE visualization of PLA2G2Ahigh fibroblasts, COL1A1high fibroblasts, TCF21high fibroblasts, and ACTA2high fibroblasts. B The heatmap showing the expression levels of top five marker genes in fibroblast subpopulations. C Relative percentage of fibroblast subpopulations in lung from the patient with FHP. D Violin plots displaying the expression levels of the representative genes in each fibroblast subpopulation. E Relative gene set enrichment scores of subpopulations calculated by AUCell analysis. F Multiplex immunofluorescence images of TCF21, ACTA2, and COL1A1 in lung tissues from FHP. Scale bar = 10 μm. G Trajectory analysis of PLA2G2Ahigh fibroblasts, COL1A1high fibroblasts, TCF21high fibroblasts, and ACTA2high fibroblasts using Monocle 2 (upper) and Velocyto R package (lower). H CEBPD_extended motif showed greater enrichment of regulon activity for PLA2G2Ahigh fibroblasts using SCENIC analysis (left panel). MEF2C_extended motif showed greater enrichment of regulon activity for ACTA2high fibroblasts (left panel). t-SNE visualization of AUC values of CEBPD_extended and MEF2C_extended motifs in fibroblasts (right panel). I Schematic developmental trajectories of fibroblast subpopulations in FHP lungs. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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