Kinesin 5A Antibody - #DF13467
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13467, RRID:AB_2846486.
Fold/Unfold
D12S1889; KIF 5A; Kif5a; KIF5A_HUMAN; Kinesin family member 5A; Kinesin heavy chain isoform 5A; Kinesin Heavy Chain Neuron Specific; Kinesin heavy chain neuron-specific 1; MY050; Neuronal kinesin heavy chain; NKHC 1; NKHC; SPG 10;
Immunogens
- Q12840 KIF5A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAETNNECSIKVLCRFRPLNQAEILRGDKFIPIFQGDDSVVIGGKPYVFDRVFPPNTTQEQVYHACAMQIVKDVLAGYNGTIFAYGQTSSGKTHTMEGKLHDPQLMGIIPRIARDIFNHIYSMDENLEFHIKVSYFEIYLDKIRDLLDVTKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVIDEGKSNRHVAVTNMNEHSSRSHSIFLINIKQENMETEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGTKSYVPYRDSKMTRILQDSLGGNCRTTMFICCSPSSYNDAETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEKEKTKAQKETIAKLEAELSRWRNGENVPETERLAGEEAALGAELCEETPVNDNSSIVVRIAPEERQKYEEEIRRLYKQLDDKDDEINQQSQLIEKLKQQMLDQEELLVSTRGDNEKVQRELSHLQSENDAAKDEVKEVLQALEELAVNYDQKSQEVEEKSQQNQLLVDELSQKVATMLSLESELQRLQEVSGHQRKRIAEVLNGLMKDLSEFSVIVGNGEIKLPVEISGAIEEEFTVARLYISKIKSEVKSVVKRCRQLENLQVECHRKMEVTGRELSSCQLLISQHEAKIRSLTEYMQSVELKKRHLEESYDSLSDELAKLQAQETVHEVALKDKEPDTQDADEVKKALELQMESHREAHHRQLARLRDEINEKQKTIDELKDLNQKLQLELEKLQADYEKLKSEEHEKSTKLQELTFLYERHEQSKQDLKGLEETVARELQTLHNLRKLFVQDVTTRVKKSAEMEPEDSGGIHSQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALEGALKEAKEGAMKDKRRYQQEVDRIKEAVRYKSSGKRGHSAQIAKPVRPGHYPASSPTNPYGTRSPECISYTNSLFQNYQNLYLQATPSSTSDMYFANSCTSSGATSSGGPLASYQKANMDNGNATDINDNRSDLPCGYEAEDQAKLFPLHQETAAS
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q12840 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T152 | Phosphorylation | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| T170 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| T298 | Phosphorylation | Uniprot | |
| T299 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| T317 | Phosphorylation | Uniprot | |
| T326 | Phosphorylation | Uniprot | |
| K348 | Acetylation | Uniprot | |
| S705 | Phosphorylation | Uniprot | |
| T806 | Phosphorylation | Uniprot | |
| T807 | Phosphorylation | Uniprot | |
| S831 | Phosphorylation | Uniprot | |
| K842 | Ubiquitination | Uniprot | |
| K845 | Ubiquitination | Uniprot | |
| K883 | Acetylation | Uniprot | |
| K888 | Acetylation | Uniprot | |
| K890 | Acetylation | Uniprot | |
| R899 | Methylation | Uniprot | |
| S931 | Phosphorylation | Uniprot |
Research Backgrounds
Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. The ZFYVE27-KIF5A complex contributes to the vesicular transport of VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 proteins in neurons. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation.
Cytoplasm>Perinuclear region. Cytoplasm>Cytoskeleton. Perikaryon.
Note: Concentrated in the cell body of the neurons, particularly in the perinuclear region.
Distributed throughout the CNS but is highly enriched in subsets of neurons.
Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1. Interacts with FMR1 (via C-terminus); this interaction is increased in a mGluR-dependent manner. Interacts with ZFYVE27. Interacts with VAPA, VAPB, SURF4, RAB11A (GDP-bound form), RAB11B (GDP-bound form) and RTN3 in a ZFYVE27-dependent manner (By similarity). Interacts with BORCS5. Interacts with BICD2.
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
Research Fields
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Organismal Systems > Nervous system > Dopaminergic synapse.
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