Product: T Plastin Antibody
Catalog: DF13459
Description: Rabbit polyclonal antibody to T Plastin
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 70kDa; 71kD(Calculated).
Uniprot: P13797
RRID: AB_2846478

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(86%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
T Plastin Antibody detects endogenous levels of total T Plastin.
RRID:
AB_2846478
Cite Format: Affinity Biosciences Cat# DF13459, RRID:AB_2846478.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BMND18; Plastin 3 (T isoform); Plastin 3; Plastin-3; PLS3; PLST_HUMAN; T fimbrin; T-plastin;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P13797 PLST_HUMAN:

Expressed in a variety of organs, including muscle, brain, uterus and esophagus.

Sequence:
MDEMATTQISKDELDELKEAFAKVDLNSNGFICDYELHELFKEANMPLPGYKVREIIQKLMLDGDRNKDGKISFDEFVYIFQEVKSSDIAKTFRKAINRKEGICALGGTSELSSEGTQHSYSEEEKYAFVNWINKALENDPDCRHVIPMNPNTDDLFKAVGDGIVLCKMINLSVPDTIDERAINKKKLTPFIIQENLNLALNSASAIGCHVVNIGAEDLRAGKPHLVLGLLWQIIKIGLFADIELSRNEALAALLRDGETLEELMKLSPEELLLRWANFHLENSGWQKINNFSADIKDSKAYFHLLNQIAPKGQKEGEPRIDINMSGFNETDDLKRAESMLQQADKLGCRQFVTPADVVSGNPKLNLAFVANLFNKYPALTKPENQDIDWTLLEGETREERTFRNWMNSLGVNPHVNHLYADLQDALVILQLYERIKVPVDWSKVNKPPYPKLGANMKKLENCNYAVELGKHPAKFSLVGIGGQDLNDGNQTLTLALVWQLMRRYTLNVLEDLGDGQKANDDIIVNWVNRTLSEAGKSTSIQSFKDKTISSSLAVVDLIDAIQPGCINYDLVKSGNLTEDDKHNNAKYAVSMARRIGARVYALPEDLVEVKPKMVMTVFACLMGRGMKRV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Chicken
86
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13797 As Substrate

Site PTM Type Enzyme
K11 Ubiquitination
K18 Ubiquitination
Y35 Phosphorylation
K42 Ubiquitination
K52 Ubiquitination
K59 Ubiquitination
R66 Methylation
K85 Ubiquitination
K91 Ubiquitination
K100 Ubiquitination
T117 Phosphorylation
Y121 Phosphorylation
K126 Ubiquitination
Y127 Phosphorylation
K135 Ubiquitination
R144 Methylation
K158 Ubiquitination
K168 Ubiquitination
S173 Phosphorylation
K185 Acetylation
S203 Phosphorylation
S205 Phosphorylation
S246 Phosphorylation
K266 Ubiquitination
S268 Phosphorylation
S293 Phosphorylation
K297 Ubiquitination
K300 Ubiquitination
Y302 Phosphorylation
S326 Phosphorylation
K335 Acetylation
K335 Ubiquitination
S339 Phosphorylation
K346 Ubiquitination
T354 Phosphorylation
S360 Phosphorylation
K364 Ubiquitination
K382 Ubiquitination
T391 Phosphorylation
K437 Ubiquitination
K444 Methylation
K447 Ubiquitination
K452 Ubiquitination
K459 Acetylation
K459 Ubiquitination
K471 Ubiquitination
S477 Phosphorylation
T492 Phosphorylation
K537 Ubiquitination
K545 Methylation
K545 Ubiquitination
K587 Ubiquitination
Y601 Phosphorylation
K611 Ubiquitination
K613 Ubiquitination
T617 Phosphorylation

Research Backgrounds

Function:

Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. May play a role in the regulation of bone development.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in a variety of organs, including muscle, brain, uterus and esophagus.

Subunit Structure:

Monomer.

Restrictive clause

 

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