Chd7 Antibody - #DF13453

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Product: Chd7 Antibody
Catalog: DF13453
Description: Rabbit polyclonal antibody to Chd7
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 335kDa; 336kD(Calculated).
Uniprot: Q9P2D1
RRID: AB_2846472

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Chd7 Antibody detects endogenous levels of total Chd7.
RRID:
AB_2846472
Cite Format: Affinity Biosciences Cat# DF13453, RRID:AB_2846472.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP-dependent helicase CHD7; ATP-dependent helicase chromodomain helicase DNA binding protein 7; CHD-7; Chd7; CHD7_HUMAN; Chromodomain helicase DNA binding protein 7; chromodomain helicase DNA binding protein 7 isoform CRA_e; Chromodomain-helicase-DNA-binding protein 7; FLJ20357; FLJ20361; HH5; IS3; KAL5; KIAA1416;

Immunogens

Immunogen:
Uniprot:

Q9P2D1(CHD7_HUMAN) >>Visit HPA database.

Gene(ID):
CHD7(55636)
Expression:
Q9P2D1 CHD7_HUMAN:

Widely expressed in fetal and adult tissues.

Sequence:
MADPGMMSLFGEDGNIFSEGLEGLGECGYPENPVNPMGQQMPIDQGFASLQPSLHHPSTNQNQTKLTHFDHYNQYEQQKMHLMDQPNRMMSNTPGNGLASPHSQYHTPPVPQVPHGGSGGGQMGVYPGMQNERHGQSFVDSSSMWGPRAVQVPDQIRAPYQQQQPQPQPPQPAPSGPPAQGHPQHMQQMGSYMARGDFSMQQHGQPQQRMSQFSQGQEGLNQGNPFIATSGPGHLSHVPQQSPSMAPSLRHSVQQFHHHPSTALHGESVAHSPRFSPNPPQQGAVRPQTLNFSSRSQTVPSPTINNSGQYSRYPYSNLNQGLVNNTGMNQNLGLTNNTPMNQSVPRYPNAVGFPSNSGQGLMHQQPIHPSGSLNQMNTQTMHPSQPQGTYASPPPMSPMKAMSNPAGTPPPQVRPGSAGIPMEVGSYPNMPHPQPSHQPPGAMGIGQRNMGPRNMQQSRPFIGMSSAPRELTGHMRPNGCPGVGLGDPQAIQERLIPGQQHPGQQPSFQQLPTCPPLQPHPGLHHQSSPPHPHHQPWAQLHPSPQNTPQKVPVHQHSPSEPFLEKPVPDMTQVSGPNAQLVKSDDYLPSIEQQPQQKKKKKKNNHIVAEDPSKGFGKDDFPGGVDNQELNRNSLDGSQEEKKKKKRSKAKKDPKEPKEPKEKKEPKEPKTPKAPKIPKEPKEKKAKTATPKPKSSKKSSNKKPDSEASALKKKVNKGKTEGSENSDLDKTPPPSPPPEEDEDPGVQKRRSSRQVKRKRYTEDLEFKISDEEADDADAAGRDSPSNTSQSEQQESVDAEGPVVEKIMSSRSVKKQKESGEEVEIEEFYVKYKNFSYLHCQWASIEDLEKDKRIQQKIKRFKAKQGQNKFLSEIEDELFNPDYVEVDRIMDFARSTDDRGEPVTHYLVKWCSLPYEDSTWERRQDIDQAKIEEFEKLMSREPETERVERPPADDWKKSESSREYKNNNKLREYQLEGVNWLLFNWYNMRNCILADEMGLGKTIQSITFLYEIYLKGIHGPFLVIAPLSTIPNWEREFRTWTELNVVVYHGSQASRRTIQLYEMYFKDPQGRVIKGSYKFHAIITTFEMILTDCPELRNIPWRCVVIDEAHRLKNRNCKLLEGLKMMDLEHKVLLTGTPLQNTVEELFSLLHFLEPSRFPSETTFMQEFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKEETIIEVELTNIQKKYYRAILEKNFTFLSKGGGQANVPNLLNTMMELRKCCNHPYLINGAEEKILEEFKETHNAESPDFQLQAMIQAAGKLVLIDKLLPKLKAGGHRVLIFSQMVRCLDILEDYLIQRRYPYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKSVKIYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRENATNGVQQLSKKEIEDLLRKGAYGALMDEEDEGSKFCEEDIDQILLRRTHTITIESEGKGSTFAKASFVASGNRTDISLDDPNFWQKWAKKAELDIDALNGRNNLVIDTPRVRKQTRLYSAVKEDELMEFSDLESDSEEKPCAKPRRPQDKSQGYARSECFRVEKNLLVYGWGRWTDILSHGRYKRQLTEQDVETICRTILVYCLNHYKGDENIKSFIWDLITPTADGQTRALVNHSGLSAPVPRGRKGKKVKAQSTQPVVQDADWLASCNPDALFQEDSYKKHLKHHCNKVLLRVRMLYYLRQEVIGDQADKILEGADSSEADVWIPEPFHAEVPADWWDKEADKSLLIGVFKHGYEKYNSMRADPALCFLERVGMPDAKAIAAEQRGTDMLADGGDGGEFDREDEDPEYKPTRTPFKDEIDEFANSPSEDKEESMEIHATGKHSESNAELGQLYWPNTSTLTTRLRRLITAYQRSYKRQQMRQEALMKTDRRRRRPREEVRALEAEREAIISEKRQKWTRREEADFYRVVSTFGVIFDPVKQQFDWNQFRAFARLDKKSDESLEKYFSCFVAMCRRVCRMPVKPDDEPPDLSSIIEPITEERASRTLYRIELLRKIREQVLHHPQLGERLKLCQPSLDLPEWWECGRHDRDLLVGAAKHGVSRTDYHILNDPELSFLDAHKNFAQNRGAGNTSSLNPLAVGFVQTPPVISSAHIQDERVLEQAEGKVEEPENPAAKEKCEGKEEEEETDGSGKESKQECEAEASSVKNELKGVEVGADTGSKSISEKGSEEDEEEKLEDDDKSEESSQPEAGAVSRGKNFDEESNASMSTARDETRDGFYMEDGDPSVAQLLHERTFAFSFWPKDRVMINRLDNICEAVLKGKWPVNRRQMFDFQGLIPGYTPTTVDSPLQKRSFAELSMVGQASISGSEDITTSPQLSKEDALNLSVPRQRRRRRRKIEIEAERAAKRRNLMEMVAQLRESQVVSENGQEKVVDLSKASREATSSTSNFSSLSSKFILPNVSTPVSDAFKTQMELLQAGLSRTPTRHLLNGSLVDGEPPMKRRRGRRKNVEGLDLLFMSHKRTSLSAEDAEVTKAFEEDIETPPTRNIPSPGQLDPDTRIPVINLEDGTRLVGEDAPKNKDLVEWLKLHPTYTVDMPSYVPKNADVLFSSFQKPKQKRHRCRNPNKLDINTLTGEERVPVVNKRNGKKMGGAMAPPMKDLPRWLEENPEFAVAPDWTDIVKQSGFVPESMFDRLLTGPVVRGEGASRRGRRPKSEIARAAAAAAAVASTSGINPLLVNSLFAGMDLTSLQNLQNLQSLQLAGLMGFPPGLATAATAGGDAKNPAAVLPLMLPGMAGLPNVFGLGGLLNNPLSAATGNTTTASSQGEPEDSTSKGEEKGNENEDENKDSEKSTDAVSAADSANGSVGAATAPAGLPSNPLAFNPFLLSTMAPGLFYPSMFLPPGLGGLTLPGFPALAGLQNAVGSSEEKAADKAEGGPFKDGETLEGSDAEESLDKTAESSLLEDEIAQGEELDSLDGGDEIENNENDE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
75
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9P2D1 As Substrate

Site PTM Type Enzyme
Y75 Phosphorylation
R148 Methylation
R195 Methylation
S199 Phosphorylation
R209 Methylation
R414 Methylation
R448 Methylation
S557 Phosphorylation
S559 Phosphorylation
K565 Acetylation
S612 Phosphorylation
S633 Phosphorylation
S637 Phosphorylation
T689 Phosphorylation
S708 Phosphorylation
S725 Phosphorylation
T730 Phosphorylation
S734 Phosphorylation
S768 Phosphorylation
S782 Phosphorylation
T786 Phosphorylation
K928 Ubiquitination
K934 Ubiquitination
K1116 Ubiquitination
K1177 Ubiquitination
K1183 Ubiquitination
K1196 Sumoylation
T1204 Phosphorylation
Y1217 Phosphorylation
Y1218 Phosphorylation
T1227 Phosphorylation O14757 (CHEK1)
K1250 Ubiquitination
Y1256 Phosphorylation
K1297 Ubiquitination
R1308 Methylation
R1317 Methylation
R1359 Methylation
K1427 Ubiquitination
K1431 Ubiquitination
K1436 Ubiquitination
T1449 Phosphorylation
S1456 Phosphorylation
K1458 Ubiquitination
K1466 Ubiquitination
K1511 Ubiquitination
K1533 Ubiquitination
T1555 Phosphorylation
S1566 Phosphorylation
S1577 Phosphorylation
S1581 Phosphorylation
S1583 Phosphorylation
S1793 Phosphorylation
T1862 Phosphorylation
S1874 Phosphorylation
S1876 Phosphorylation
S2141 Phosphorylation
T2153 Phosphorylation
S2229 Phosphorylation
S2231 Phosphorylation
S2233 Phosphorylation
S2237 Phosphorylation
S2251 Phosphorylation
S2254 Phosphorylation
S2255 Phosphorylation
S2272 Phosphorylation
S2275 Phosphorylation
S2277 Phosphorylation
T2278 Phosphorylation
Y2349 Phosphorylation
T2350 Phosphorylation
T2352 Phosphorylation
S2356 Phosphorylation
S2395 Phosphorylation
K2446 Ubiquitination
S2471 Phosphorylation P24941 (CDK2)
T2472 Phosphorylation P24941 (CDK2)
T2492 Phosphorylation
S2501 Phosphorylation
T2532 Phosphorylation
S2533 Phosphorylation
S2535 Phosphorylation
T2542 Phosphorylation
T2551 Phosphorylation
T2554 Phosphorylation
S2559 Phosphorylation
Y2601 Phosphorylation
Y2608 Phosphorylation
S2619 Phosphorylation
T2640 Phosphorylation
S2698 Phosphorylation
T2705 Phosphorylation
S2715 Phosphorylation
T2952 Phosphorylation
S2956 Phosphorylation
S2961 Phosphorylation
T2965 Phosphorylation
S2969 Phosphorylation
S2983 Phosphorylation

Research Backgrounds

Function:

Probable transcription regulator. Maybe involved in the in 45S precursor rRNA production.

Subcellular Location:

Nucleus.

Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed in fetal and adult tissues.

Subunit Structure:

May interact with CTCF. Interacts with CHD8. Interacts with FAM124B. Found in a complex composed of AGO2, CHD7 and FAM172A (By similarity).

Family&Domains:

Belongs to the SNF2/RAD54 helicase family.

Restrictive clause

 

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