Niemann Pick C1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HepG2 cells, using Niemann Pick C1 Antibody.

Product:	Niemann Pick C1 Antibody
Catalog:	DF13452
Description:	Rabbit polyclonal antibody to Niemann Pick C1
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Rabbit, Dog
Mol.Wt.:	142kDa; 142kD(Calculated).
Uniprot:	O15118
RRID:	AB_2846471

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(86%), Horse(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Niemann Pick C1 Antibody detects endogenous levels of total Niemann Pick C1.

RRID:

AB_2846471
Cite Format: Affinity Biosciences Cat# DF13452, RRID:AB_2846471.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Niemann Pick C1 protein precursor; Niemann Pick disease, type C1; Niemann-Pick C1 protein; NPC; NPC1; NPC1_HUMAN;

Immunogens

Immunogen:

Uniprot:

O15118(NPC1_HUMAN) >>Visit HPA database.

Gene(ID):

NPC1(4864)

Sequence:

O15118 NPC1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTARGLALGLLLLLLCPAQVFSQSCVWYGECGIAYGDKRYNCEYSGPPKPLPKDGYDLVQELCPGFFFGNVSLCCDVRQLQTLKDNLQLPLQFLSRCPSCFYNLLNLFCELTCSPRQSQFLNVTATEDYVDPVTNQTKTNVKELQYYVGQSFANAMYNACRDVEAPSSNDKALGLLCGKDADACNATNWIEYMFNKDNGQAPFTITPVFSDFPVHGMEPMNNATKGCDESVDEVTAPCSCQDCSIVCGPKPQPPPPPAPWTILGLDAMYVIMWITYMAFLLVFFGAFFAVWCYRKRYFVSEYTPIDSNIAFSVNASDKGEASCCDPVSAAFEGCLRRLFTRWGSFCVRNPGCVIFFSLVFITACSSGLVFVRVTTNPVDLWSAPSSQARLEKEYFDQHFGPFFRTEQLIIRAPLTDKHIYQPYPSGADVPFGPPLDIQILHQVLDLQIAIENITASYDNETVTLQDICLAPLSPYNTNCTILSVLNYFQNSHSVLDHKKGDDFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLGTFGGPVFPWLVLGGYDDQNYNNATALVITFPVNNYYNDTEKLQRAQAWEKEFINFVKNYKNPNLTISFTAERSIEDELNRESDSDVFTVVISYAIMFLYISLALGHMKSCRRLLVDSKVSLGIAGILIVLSSVACSLGVFSYIGLPLTLIVIEVIPFLVLAVGVDNIFILVQAYQRDERLQGETLDQQLGRVLGEVAPSMFLSSFSETVAFFLGALSVMPAVHTFSLFAGLAVFIDFLLQITCFVSLLGLDIKRQEKNRLDIFCCVRGAEDGTSVQASESCLFRFFKNSYSPLLLKDWMRPIVIAIFVGVLSFSIAVLNKVDIGLDQSLSMPDDSYMVDYFKSISQYLHAGPPVYFVLEEGHDYTSSKGQNMVCGGMGCNNDSLVQQIFNAAQLDNYTRIGFAPSSWIDDYFDWVKPQSSCCRVDNITDQFCNASVVDPACVRCRPLTPEGKQRPQGGDFMRFLPMFLSDNPNPKCGKGGHAAYSSAVNILLGHGTRVGATYFMTYHTVLQTSADFIDALKKARLIASNVTETMGINGSAYRVFPYSVFYVFYEQYLTIIDDTIFNLGVSLGAIFLVTMVLLGCELWSAVIMCATIAMVLVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTVSMKGSRVERAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFQIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKSCATEERYKGTERERLLNF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

100

Rabbit

100

Pig

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15118 As Substrate

O15118

Site	PTM Type	Source
K49	Ubiquitination	Uniprot
N70	N-Glycosylation	Uniprot
K84	Ubiquitination	Uniprot
S95	Phosphorylation	Uniprot
N122	N-Glycosylation	Uniprot
N135	N-Glycosylation	Uniprot
N158	N-Glycosylation	Uniprot
K171	Ubiquitination	Uniprot
N185	N-Glycosylation	Uniprot
N222	N-Glycosylation	Uniprot
Y302	Phosphorylation	Uniprot
S382	Phosphorylation	Uniprot
S385	Phosphorylation	Uniprot
S386	Phosphorylation	Uniprot
K392	Ubiquitination	Uniprot
Y394	Phosphorylation	Uniprot
N452	N-Glycosylation	Uniprot
N459	N-Glycosylation	Uniprot
N478	N-Glycosylation	Uniprot
N524	N-Glycosylation	Uniprot
N557	N-Glycosylation	Uniprot
N572	N-Glycosylation	Uniprot
K585	Ubiquitination	Uniprot
N598	N-Glycosylation	Uniprot
K822	Ubiquitination	Uniprot
S826	Phosphorylation	Uniprot
K903	Acetylation	Uniprot
N916	N-Glycosylation	Uniprot
N931	N-Glycosylation	Uniprot
N961	N-Glycosylation	Uniprot
N968	N-Glycosylation	Uniprot
K987	Ubiquitination	Uniprot
N1064	N-Glycosylation	Uniprot
K1180	Ubiquitination	Uniprot
T1270	Phosphorylation	Uniprot

Research Backgrounds

O15118_NPC1_HUMAN

Function:

Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals (Probable).

(Microbial infection) Acts as an endosomal entry receptor for ebolavirus.

PTMs:

N-glycosylated.

Subcellular Location:

Late endosome membrane>Multi-pass membrane protein. Lysosome membrane>Multi-pass membrane protein.

Subunit Structure:

Interacts (via the second lumenal domain) with NPC2. Interacts with TMEM97. Interacts with TIM1.

(Microbial infection) Interacts with ebolavirus glycoprotein.

Family&Domains:

A cysteine-rich N-terminal domain and a C-terminal domain containing a di-leucine motif necessary for lysosomal targeting are critical for mobilization of cholesterol from lysosomes.

Belongs to the patched family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)

· Organismal Systems > Digestive system > Cholesterol metabolism.

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Niemann Pick C1 Antibody - #DF13452