KCNQ2 Antibody - #DF13451
| Product: | KCNQ2 Antibody |
| Catalog: | DF13451 |
| Description: | Rabbit polyclonal antibody to KCNQ2 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Horse, Chicken |
| Mol.Wt.: | 95kDa; 96kD(Calculated). |
| Uniprot: | O43526 |
| RRID: | AB_2846470 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13451, RRID:AB_2846470.
Immunogens
In adult and fetal brain. Highly expressed in areas containing neuronal cell bodies, low in spinal chord and corpus callosum. Isoform 2 is preferentially expressed in differentiated neurons. Isoform 6 is prominent in fetal brain, undifferentiated neuroblastoma cells and brain tumors.
- O43526 KCNQ2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVQKSRNGGVYPGPSGEKKLKVGFVGLDPGAPDSTRDGALLIAGSEAPKRGSILSKPRAGGAGAGKPPKRNAFYRKLQNFLYNVLERPRGWAFIYHAYVFLLVFSCLVLSVFSTIKEYEKSSEGALYILEIVTIVVFGVEYFVRIWAAGCCCRYRGWRGRLKFARKPFCVIDIMVLIASIAVLAAGSQGNVFATSALRSLRFLQILRMIRMDRRGGTWKLLGSVVYAHSKELVTAWYIGFLCLILASFLVYLAEKGENDHFDTYADALWWGLITLTTIGYGDKYPQTWNGRLLAATFTLIGVSFFALPAGILGSGFALKVQEQHRQKHFEKRRNPAAGLIQSAWRFYATNLSRTDLHSTWQYYERTVTVPMYSSQTQTYGASRLIPPLNQLELLRNLKSKSGLAFRKDPPPEPSPSKGSPCRGPLCGCCPGRSSQKVSLKDRVFSSPRGVAAKGKGSPQAQTVRRSPSADQSLEDSPSKVPKSWSFGDRSRARQAFRIKGAASRQNSEEASLPGEDIVDDKSCPCEFVTEDLTPGLKVSIRAVCVMRFLVSKRKFKESLRPYDVMDVIEQYSAGHLDMLSRIKSLQSRVDQIVGRGPAITDKDRTKGPAEAELPEDPSMMGRLGKVEKQVLSMEKKLDFLVNIYMQRMGIPPTETEAYFGAKEPEPAPPYHSPEDSREHVDRHGCIVKIVRSSSSTGQKNFSAPPAAPPVQCPPSTSWQPQSHPRQGHGTSPVGDHGSLVRIPPPPAHERSLSAYGGGNRASMEFLRQEDTPGCRPPEGNLRDSDTSISIPSVDHEELERSFSGFSISQSKENLDALNSCYAAVAPCAKVRPYIAEGESDTDSDLCTPCGPPPRSATGEGPFGDVGWAGPRK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O43526 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K21 | Ubiquitination | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| S52 | Phosphorylation | Uniprot | |
| T217 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| Y237 | Phosphorylation | Uniprot | |
| Y372 | Phosphorylation | Uniprot | |
| Y379 | Phosphorylation | Uniprot | |
| K398 | Acetylation | Uniprot | |
| S438 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| S507 | Phosphorylation | Uniprot | |
| K556 | Methylation | Uniprot | |
| Y562 | Phosphorylation | Uniprot | |
| Y571 | Phosphorylation | Uniprot | |
| Y644 | Phosphorylation | Uniprot | |
| Y658 | Phosphorylation | Uniprot | |
| Y755 | Phosphorylation | Uniprot | |
| S762 | Phosphorylation | Uniprot | |
| Y821 | Phosphorylation | Uniprot | |
| S843 | Phosphorylation | Uniprot | |
| S855 | Phosphorylation | Uniprot | |
| T857 | Phosphorylation | Uniprot |
Research Backgrounds
Associates with KCNQ3 to form a potassium channel with essentially identical properties to the channel underlying the native M-current, a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons as well as the responsiveness to synaptic inputs. Therefore, it is important in the regulation of neuronal excitability. KCNQ2/KCNQ3 current is blocked by linopirdine and XE991, and activated by the anticonvulsant retigabine. As the native M-channel, the potassium channel composed of KCNQ2 and KCNQ3 is also suppressed by activation of the muscarinic acetylcholine receptor CHRM1.
KCNQ2/KCNQ3 heteromeric current can be increased by intracellular cyclic AMP, an effect that depends on phosphorylation of Ser-52 in the N-terminal region.
KCNQ2/KCNQ3 are ubiquitinated by NEDD4L. Ubiquitination leads to protein degradation (Probable). Degradation induced by NEDD4L is inhibited by USP36.
Cell membrane>Multi-pass membrane protein.
In adult and fetal brain. Highly expressed in areas containing neuronal cell bodies, low in spinal chord and corpus callosum. Isoform 2 is preferentially expressed in differentiated neurons. Isoform 6 is prominent in fetal brain, undifferentiated neuroblastoma cells and brain tumors.
Heterotetramer with KCNQ3; form the heterotetrameric M potassium channel. Interacts with calmodulin; the interaction is calcium-independent, constitutive and participates to the proper assembly of a functional heterotetrameric M channel. May associate with KCNE2. Interacts with IQCJ-SCHIP1 (By similarity).
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.2/KCNQ2 sub-subfamily.
Research Fields
· Organismal Systems > Nervous system > Cholinergic synapse.
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