STAM1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Pc12, using STAM1 Antibody.

Product:	STAM1 Antibody
Catalog:	DF13448
Description:	Rabbit polyclonal antibody to STAM1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	59kDa; 59kD(Calculated).
Uniprot:	Q92783
RRID:	AB_2846467

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

STAM1 Antibody detects endogenous levels of total STAM1.

RRID:

AB_2846467
Cite Format: Affinity Biosciences Cat# DF13448, RRID:AB_2846467.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

HSE1 homolog; Signal transducing adapter molecule 1; signal transducing adaptor molecule (SH3 domain and ITAM motif) 1; STAM; STAM-1; STAM1_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q92783(STAM1_HUMAN) >>Visit HPA database.

Gene(ID):

STAM(8027)

Expression:

Q92783 STAM1_HUMAN:

Ubiquitously expressed.

Sequence:

Q92783 STAM1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPLFATNPFDQDVEKATSEMNTAEDWGLILDICDKVGQSRTGPKDCLRSIMRRVNHKDPHVAMQALTLLGACVSNCGKIFHLEVCSRDFASEVSNVLNKGHPKVCEKLKALMVEWTDEFKNDPQLSLISAMIKNLKEQGVTFPAIGSQAAEQAKASPALVAKDPGTVANKKEEEDLAKAIELSLKEQRQQSTTLSTLYPSTSSLLTNHQHEGRKVRAIYDFEAAEDNELTFKAGEIITVLDDSDPNWWKGETHQGIGLFPSNFVTADLTAEPEMIKTEKKTVQFSDDVQVETIEPEPEPAFIDEDKMDQLLQMLQSTDPSDDQPDLPELLHLEAMCHQMGPLIDEKLEDIDRKHSELSELNVKVMEALSLYTKLMNEDPMYSMYAKLQNQPYYMQSSGVSGSQVYAGPPPSGAYLVAGNAQMSHLQSYSLPPEQLSSLSQAVVPPSANPALPSQQTQAAYPNTMVSSVQGNTYPSQAPVYSPPPAATAAAATADVTLYQNAGPNMPQVPNYNLTSSTLPQPGGSQQPPQPQQPYSQKALL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92783 As Substrate

Q92783

Site	PTM Type	Source
S91	Phosphorylation	Uniprot
K99	Ubiquitination	Uniprot
K103	Ubiquitination	Uniprot
K133	Ubiquitination	Uniprot
K136	Ubiquitination	Uniprot
K154	Ubiquitination	Uniprot
S156	Phosphorylation	Uniprot
K162	Ubiquitination	Uniprot
K170	Ubiquitination	Uniprot
K171	Ubiquitination	Uniprot
K178	Ubiquitination	Uniprot
S183	Phosphorylation	Uniprot
K185	Ubiquitination	Uniprot
T196	Phosphorylation	Uniprot
Y198	Phosphorylation	Uniprot
S200	Phosphorylation	Uniprot
T201	Phosphorylation	Uniprot
S202	Phosphorylation	Uniprot
S203	Phosphorylation	Uniprot
T206	Phosphorylation	Uniprot
K276	Ubiquitination	Uniprot
K353	Ubiquitination	Uniprot
S355	Phosphorylation	Uniprot
Y371	Phosphorylation	Uniprot
T372	Phosphorylation	Uniprot
Y381	Phosphorylation	Uniprot
S382	Phosphorylation	Uniprot
Y384	Phosphorylation	Uniprot

Research Backgrounds

Q92783_STAM1_HUMAN

Function:

Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.

PTMs:

Phosphorylated on Tyr-198. Phosphorylated in response to IL2, IL3, IL4, IL7, CSF2/GM-CSF, EGF and PDGFB. Phosphorylated by activated PDGFRB.

Ubiquitinated by ITCH.

Subcellular Location:

Cytoplasm. Early endosome membrane>Peripheral membrane protein>Cytoplasmic side.

Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS. Probably part of a complex at least composed of HSG, STAM and EPS15. Found in a complex with HGS and E3 ligase ITCH and DTX3L. Interacts with E3 ligase DTX3L; the interaction brings together STAM and HSG, promotes their recruitment to early endosomes and decreases STAM and HGS ubiquitination by ITCH. Interacts with STAMBP/AMSH. Interacts with PDGFRB. Interacts with LITAF; the interaction is direct. Identified in a complex with HGS and LITAF.

Family&Domains:

The VHS domain mediates high-avidity binding to Lys63-linked and Lys48-linked polyubiquitinated cargos.

Belongs to the STAM family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

STAM1 Antibody - #DF13448