PGAM2 Antibody - #DF13447

(4)  
Product: PGAM2 Antibody
Catalog: DF13447
Description: Rabbit polyclonal antibody to PGAM2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 28kDa; 29kD(Calculated).
Uniprot: P15259
RRID: AB_2846466

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(88%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
PGAM2 Antibody detects endogenous levels of total PGAM2.
RRID:
AB_2846466
Cite Format: Affinity Biosciences Cat# DF13447, RRID:AB_2846466.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BPG dependent PGAM 2; BPG-dependent PGAM 2; GSD10; MGC88743; Muscle specific phosphoglycerate mutase; Muscle-specific phosphoglycerate mutase; OTTHUMP00000207787; PGAM 2; PGAM M; PGAM-M; Pgam2; PGAM2_HUMAN; PGAMM; phosphoglycerate mutase 2 (muscle); Phosphoglycerate mutase 2; Phosphoglycerate mutase isozyme M;

Immunogens

Immunogen:
Uniprot:

P15259(PGAM2_HUMAN) >>Visit HPA database.

Gene(ID):
PGAM2(5224)
Expression:
P15259 PGAM2_HUMAN:

Expressed in the heart and muscle. Not found in the liver and brain.

Sequence:
MATHRLVMVRHGESTWNQENRFCGWFDAELSEKGTEEAKRGAKAIKDAKMEFDICYTSVLKRAIRTLWAILDGTDQMWLPVVRTWRLNERHYGGLTGLNKAETAAKHGEEQVKIWRRSFDIPPPPMDEKHPYYNSISKERRYAGLKPGELPTCESLKDTIARALPFWNEEIVPQIKAGKRVLIAAHGNSLRGIVKHLEGMSDQAIMELNLPTGIPIVYELNKELKPTKPMQFLGDEETVRKAMEAVAAQGKAK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Bovine
88
Sheep
88
Zebrafish
88
Pig
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15259 As Substrate

Site PTM Type Enzyme
T3 Phosphorylation
K33 Acetylation
K39 Acetylation
R90 Methylation
Y92 Phosphorylation
T96 Phosphorylation
K100 Acetylation
K100 Sumoylation
K100 Ubiquitination
K113 Ubiquitination
S118 Phosphorylation
Y132 Phosphorylation
Y133 Phosphorylation
S135 Phosphorylation
S137 Phosphorylation
T152 Phosphorylation
K179 Ubiquitination
S189 Phosphorylation
K195 Ubiquitination
K241 Methylation
K241 Ubiquitination
K251 Ubiquitination

Research Backgrounds

Function:

Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.

Tissue Specificity:

Expressed in the heart and muscle. Not found in the liver and brain.

Subunit Structure:

Homodimer. Interacts with ENO1.

Family&Domains:

Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.

Research Fields

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Amino acid metabolism > Glycine, serine and threonine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.