CAT1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HepG2 cells, using CAT1 Antibody.

DF13433 at 1/100 staining Human liver cancer and adjacent normal tissues by IHC-P.

Product:	CAT1 Antibody
Catalog:	DF13433
Description:	Rabbit polyclonal antibody to CAT1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	67kDa; 68kD(Calculated).
Uniprot:	P30825
RRID:	AB_2846452

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(88%), Dog(88%)

Clonality:

Polyclonal

Specificity:

CAT1 Antibody detects endogenous levels of total CAT1.

RRID:

AB_2846452
Cite Format: Affinity Biosciences Cat# DF13433, RRID:AB_2846452.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Amino acid transporter cationic 1; ATRC1; CAT-1; CaT1; CTR1_HUMAN; Ecotropic retroviral leukemia receptor homolog; Ecotropic retroviral receptor; Ecotropic retrovirus receptor homolog; ERR; HCAT1; High affinity cationic amino acid transporter 1; High-affinity cationic amino acid transporter-1; REC1L; Slc7a1; Solute carrier family 7 (cationic amino acid transporter, y+ system) member 1; Solute carrier family 7 member 1; System Y+ basic amino acid transporter;

Immunogens

Immunogen:

Uniprot:

P30825(SL7A1_HUMAN) >>Visit HPA database.

Gene(ID):

SLC7A1(6541)

Expression:

P30825 SL7A1_HUMAN:

Ubiquitous.

Sequence:

P30825 SL7A1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGCKVLLNIGQQMLRRKVVDCSREETRLSRCLNTFDLVALGVGSTLGAGVYVLAGAVARENAGPAIVISFLIAALASVLAGLCYGEFGARVPKTGSAYLYSYVTVGELWAFITGWNLILSYIIGTSSVARAWSATFDELIGRPIGEFSRTHMTLNAPGVLAENPDIFAVIIILILTGLLTLGVKESAMVNKIFTCINVLVLGFIMVSGFVKGSVKNWQLTEEDFGNTSGRLCLNNDTKEGKPGVGGFMPFGFSGVLSGAATCFYAFVGFDCIATTGEEVKNPQKAIPVGIVASLLICFIAYFGVSAALTLMMPYFCLDNNSPLPDAFKHVGWEGAKYAVAVGSLCALSASLLGSMFPMPRVIYAMAEDGLLFKFLANVNDRTKTPIIATLASGAVAAVMAFLFDLKDLVDLMSIGTLLAYSLVAACVLVLRYQPEQPNLVYQMASTSDELDPADQNELASTNDSQLGFLPEAEMFSLKTILSPKNMEPSKISGLIVNISTSLIAVLIITFCIVTVLGREALTKGALWAVFLLAGSALLCAVVTGVIWRQPESKTKLSFKVPFLPVLPILSIFVNVYLMMQLDQGTWVRFAVWMLIGFIIYFGYGLWHSEEASLDADQARTPDGNLDQCK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Rabbit

Chicken

Zebrafish

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30825 As Substrate

P30825

Site	PTM Type	Source
K4	Ubiquitination	Uniprot
K215	Ubiquitination	Uniprot
N226	N-Glycosylation	Uniprot
K373	Ubiquitination	Uniprot
Y441	Phosphorylation	Uniprot
K484	Ubiquitination	Uniprot
T620	Phosphorylation	Uniprot
C628	S-Nitrosylation	Uniprot
K629	Sumoylation	Uniprot
K629	Ubiquitination	Uniprot

Research Backgrounds

P30825_SL7A1_HUMAN

Function:

High-affinity, low capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine) in non-hepatic tissues.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with TM4SF5; the interaction is negatively regulated by arginine.

Family&Domains:

Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.

Research Fields

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

References

1). Diterpenoid alkaloids isolated from Delphinium trichophorum alleviate pulmonary fibrosis via the TGF-β/Smad pathway in 3T6 and HFL-1 cells. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2022 (PubMed: 36068772) [IF=7.5]

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CAT1 Antibody - #DF13433