TBL1 Antibody | Affinity Biosciences

1/2

Western blot analysis of extracts from Hela cells, using TBL1 Antibody.

DF13425 at 1/100 staining Mouse lung tissue by IHC-P.

Product:	TBL1 Antibody
Catalog:	DF13425
Description:	Rabbit polyclonal antibody to TBL1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	62kDa; 62kD(Calculated).
Uniprot:	O60907
RRID:	AB_2846444

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

TBL1 Antibody detects endogenous levels of total TBL1.

RRID:

AB_2846444
Cite Format: Affinity Biosciences Cat# DF13425, RRID:AB_2846444.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EBI; F box like/WD repeat protein TBL1X; F-box-like/WD repeat-containing protein TBL1X; SMAP 55; SMAP55; TBL 1; TBL 1X; TBL1; TBL1X; TBL1X_HUMAN; Transducin (beta) like 1; Transducin (beta) like 1 X linked; Transducin (beta) like 1X linked; Transducin beta like 1 X linked; Transducin beta like 1X; Transducin beta like 1X protein; Transducin beta like protein 1, X linked; Transducin beta-like protein 1X; Transducin-beta-like protein 1; X-linked;

Immunogens

Immunogen:

Uniprot:

O60907(TBL1X_HUMAN) >>Visit HPA database.

Gene(ID):

TBL1X(6907)

Expression:

O60907 TBL1X_HUMAN:

Ubiquitous.

Sequence:

O60907 TBL1X_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTELAGASSSCCHRPAGRGAMQSVLHHFQRLRGREGGSHFINTSSPRGEAKMSITSDEVNFLVYRYLQESGFSHSAFTFGIESHISQSNINGTLVPPAALISILQKGLQYVEAEISINEDGTVFDGRPIESLSLIDAVMPDVVQTRQQAFREKLAQQQASAAAAAAAATAAATAATTTSAGVSHQNPSKNREATVNGEENRAHSVNNHAKPMEIDGEVEIPSSKATVLRGHESEVFICAWNPVSDLLASGSGDSTARIWNLNENSNGGSTQLVLRHCIREGGHDVPSNKDVTSLDWNTNGTLLATGSYDGFARIWTEDGNLASTLGQHKGPIFALKWNRKGNYILSAGVDKTTIIWDAHTGEAKQQFPFHSAPALDVDWQNNTTFASCSTDMCIHVCRLGCDRPVKTFQGHTNEVNAIKWDPSGMLLASCSDDMTLKIWSMKQEVCIHDLQAHNKEIYTIKWSPTGPATSNPNSNIMLASASFDSTVRLWDIERGVCTHTLTKHQEPVYSVAFSPDGKYLASGSFDKCVHIWNTQSGNLVHSYRGTGGIFEVCWNARGDKVGASASDGSVCVLDLRK

PTMs - O60907 As Substrate

O60907

Site	PTM Type	Source
T43	Phosphorylation	Uniprot
T55	Phosphorylation	Uniprot
S160	Phosphorylation	Uniprot
T169	Phosphorylation	Uniprot
T173	Phosphorylation	Uniprot
T176	Phosphorylation	Uniprot
T177	Phosphorylation	Uniprot
S223	Phosphorylation	Uniprot
T226	Phosphorylation	Uniprot
K329	Ubiquitination	Uniprot
K340	Ubiquitination	Uniprot
T383	Phosphorylation	Uniprot
K442	Ubiquitination	Uniprot
S470	Phosphorylation	Uniprot
S474	Phosphorylation	Uniprot
Y509	Phosphorylation	Uniprot
K518	Ubiquitination	Uniprot
K527	Methylation	Uniprot
K560	Sumoylation	Uniprot

Research Backgrounds

O60907_TBL1X_HUMAN

Function:

F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of corepressor complexes that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of transcription repressor complexes, thereby allowing cofactor exchange.

Subcellular Location:

Nucleus.
Note: Colocalized with MECP2 to the heterochromatin foci.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Homotetramer; dimer of dimers. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2. Interacts with GPS2 (when sumoylated); leading to protect GPS2 against degradation by the proteasome. Component of a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X. Probably part of other corepressor complexes, that do not contain NCOR1 and NCOR2. Interacts with histones H2B, H3a and H4. Interacts with MECP2; recruits TBL1X to the heterochromatin foci (By similarity).

Family&Domains:

The F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes.

Belongs to the WD repeat EBI family.

Research Fields

· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

TBL1 Antibody - #DF13425