TEM8 Antibody - #DF13424

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Product: TEM8 Antibody
Catalog: DF13424
Description: Rabbit polyclonal antibody to TEM8
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 62kDa; 63kD(Calculated).
Uniprot: Q9H6X2
RRID: AB_2846443

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(88%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
TEM8 Antibody detects endogenous levels of total TEM8.
RRID:
AB_2846443
Cite Format: Affinity Biosciences Cat# DF13424, RRID:AB_2846443.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Anthrax toxin receptor 1; ANTR1_HUMAN; Antxr1; ATR; Tumor endothelial marker 8;

Immunogens

Immunogen:
Uniprot:

Q9H6X2(ANTR1_HUMAN) >>Visit HPA database.

Gene(ID):
ANTXR1(84168)
Expression:
Q9H6X2 ANTR1_HUMAN:

Detected in umbilical vein endothelial cells (at protein level). Highly expressed in tumor endothelial cells.

Sequence:
MATAERRALGIGFQWLSLATLVLICAGQGGRREDGGPACYGGFDLYFILDKSGSVLHHWNEIYYFVEQLAHKFISPQLRMSFIVFSTRGTTLMKLTEDREQIRQGLEELQKVLPGGDTYMHEGFERASEQIYYENRQGYRTASVIIALTDGELHEDLFFYSEREANRSRDLGAIVYCVGVKDFNETQLARIADSKDHVFPVNDGFQALQGIIHSILKKSCIEILAAEPSTICAGESFQVVVRGNGFRHARNVDRVLCSFKINDSVTLNEKPFSVEDTYLLCPAPILKEVGMKAALQVSMNDGLSFISSSVIITTTHCSDGSILAIALLILFLLLALALLWWFWPLCCTVIIKEVPPPPAEESEEEDDDGLPKKKWPTVDASYYGGRGVGGIKRMEVRWGEKGSTEEGAKLEKAKNARVKMPEQEYEFPEPRNLNNNMRRPSSPRKWYSPIKGKLDALWVLLRKGYDRVSVMRPQPGDTGRCINFTRVKNNQPAKYPLNNAYHTSSPPPAPIYTPPPPAPHCPPPPPSAPTPPIPSPPSTLPPPPQAPPPNRAPPPSRPPPRPSV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Rabbit
100
Dog
88
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H6X2 As Substrate

Site PTM Type Enzyme
T3 Phosphorylation
S75 Phosphorylation
Y119 Phosphorylation
Y132 Phosphorylation
Y133 Phosphorylation
Y139 Phosphorylation
N184 N-Glycosylation
K195 Ubiquitination
K270 Ubiquitination
S362 Phosphorylation
K374 Ubiquitination
T377 Phosphorylation
S381 Phosphorylation
Y382 Phosphorylation
Y383 Phosphorylation
K401 Ubiquitination
K409 Ubiquitination
K419 Ubiquitination
Y425 Phosphorylation
K445 Ubiquitination
K451 Ubiquitination
K453 Ubiquitination
K488 Ubiquitination
Y501 Phosphorylation
T503 Phosphorylation
S563 Phosphorylation

Research Backgrounds

Function:

Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cell projection>Lamellipodium membrane>Single-pass type I membrane protein. Cell projection>Filopodium membrane>Single-pass type I membrane protein.
Note: At the membrane of lamellipodia and at the tip of actin-enriched filopodia. Colocalizes with actin at the base of lamellipodia.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in umbilical vein endothelial cells (at protein level). Highly expressed in tumor endothelial cells.

Subunit Structure:

Interacts with gelatin and type 1 collagen. Interacts with the actin cytoskeleton. Binds to the protective antigen (PA) of Bacillus anthracis. Binding does not occur in the presence of calcium.

Family&Domains:

Binding to PA occurs through the VWA domain.

Belongs to the ATR family.

Research Fields

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

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