alpha 1 Spectrin Antibody - #DF13417
(4)
| Product: | alpha 1 Spectrin Antibody |
| Catalog: | DF13417 |
| Description: | Rabbit polyclonal antibody to alpha 1 Spectrin |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Horse |
| Mol.Wt.: | 280kDa, 110 kDa; 280kD(Calculated). |
| Uniprot: | P02549 |
| RRID: | AB_2846436 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Horse(100%)
Clonality:
Polyclonal
Specificity:
alpha 1 Spectrin Antibody detects endogenous levels of total alpha 1 Spectrin.
RRID:
AB_2846436
Cite Format: Affinity Biosciences Cat# DF13417, RRID:AB_2846436.
Cite Format: Affinity Biosciences Cat# DF13417, RRID:AB_2846436.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Alpha I spectrin; EL 2; EL2; Elliptocytosis 2; Elliptocytosis2; Erythrocyte alpha spectrin; erythrocyte; Erythroid alpha spectrin; Erythroid alpha-spectrin; Erythroid spectrin alpha; HPP; HS3; Spectrin alpha chain; Spectrin alpha chain erythrocyte; Spectrin alpha erythrocytic 1; SPH3; SPTA 1; SPTA; SPTA1; SPTA1_HUMAN;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Sequence:
- P02549 SPTA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEQFPKETVVESSGPKVLETAEEIQERRQEVLTRYQSFKERVAERGQKLEDSYHLQVFKRDADDLGKWIMEKVNILTDKSYEDPTNIQGKYQKHQSLEAEVQTKSRLMSELEKTREERFTMGHSAHEETKAHIEELRHLWDLLLELTLEKGDQLLRALKFQQYVQECADILEWIGDKEAIATSVELGEDWERTEVLHKKFEDFQVELVAKEGRVVEVNQYANECAEENHPDLPLIQSKQNEVNAAWERLRGLALQRQKALSNAANLQRFKRDVTEAIQWIKEKEPVLTSEDYGKDLVASEGLFHSHKGLERNLAVMSDKVKELCAKAEKLTLSHPSDAPQIQEMKEDLVSSWEHIRALATSRYEKLQATYWYHRFSSDFDELSGWMNEKTAAINADELPTDVAGGEVLLDRHQQHKHEIDSYDDRFQSADETGQDLVNANHEASDEVREKMEILDNNWTALLELWDERHRQYEQCLDFHLFYRDSEQVDSWMSRQEAFLENEDLGNSLGSAEALLQKHEDFEEAFTAQEEKIITVDKTATKLIGDDHYDSENIKAIRDGLLARRDALREKAATRRRLLKESLLLQKLYEDSDDLKNWINKKKKLADDEDYKDIQNLKSRVQKQQVFEKELAVNKTQLENIQKTGQEMIEGGHYASDNVTTRLSEVASLWEELLEATKQKGTQLHEANQQLQFENNAEDLQRWLEDVEWQVTSEDYGKGLAEVQNRLRKHGLLESAVAARQDQVDILTDLAAYFEEIGHPDSKDIRARQESLVCRFEALKEPLATRKKKLLDLLHLQLICRDTEDEEAWIQETEPSATSTYLGKDLIASKKLLNRHRVILENIASHEPRIQEITERGNKMVEEGHFAAEDVASRVKSLNQNMESLRARAARRQNDLEANVQFQQYLADLHEAETWIREKEPIVDNTNYGADEEAAGALLKKHEAFLLDLNSFGDSMKALRNQANACQQQQAAPVEGVAGEQRVMALYDFQARSPREVTMKKGDVLTLLSSINKDWWKVEAADHQGIVPAVYVRRLAHDEFPMLPQRRREEPGNITQRQEQIENQYRSLLDRAEERRRRLLQRYNEFLLAYEAGDMLEWIQEKKAENTGVELDDVWELQKKFDEFQKDLNTNEPRLRDINKVADDLLFEGLLTPEGAQIRQELNSRWGSLQRLADEQRQLLGSAHAVEVFHREADDTKEQIEKKCQALSAADPGSDLFSVQALQRRHEGFERDLVPLGDKVTILGETAERLSESHPDATEDLQRQKMELNEAWEDLQGRTKDRKESLNEAQKFYLFLSKARDLQNWISSIGGMVSSQELAEDLTGIEILLERHQEHRADMEAEAPTFQALEDFSAELIDSGHHASPEIEKKLQAVKLERDDLEKAWEKRKKILDQCLELQMFQGNCDQVESWMVARENSLRSDDKSSLDSLEALMKKRDDLDKAITAQEGKITDLEHFAESLIADEHYAKEEIATRLQRVLDRWKALKAQLIDERTKLGDYANLKQFYRDLEELEEWISEMLPTACDESYKDATNIQRKYLKHQTFAHEVDGRSEQVHGVINLGNSLIECSACDGNEEAMKEQLEQLKEHWDHLLERTNDKGKKLNEASRQQRFNTSIRDFEFWLSEAETLLAMKDQARDLASAGNLLKKHQLLEREMLAREDALKDLNTLAEDLLSSGTFNVDQIVKKKDNVNKRFLNVQELAAAHHEKLKEAYALFQFFQDLDDEESWIEEKLIRVSSQDYGRDLQGVQNLLKKHKRLEGELVAHEPAIQNVLDMAEKLKDKAAVGQEEIQLRLAQFVEHWEKLKELAKARGLKLEESLEYLQFMQNAEEEEAWINEKNALAVRGDCGDTLAATQSLLMKHEALENDFAVHETRVQNVCAQGEDILNKVLQEESQNKEISSKIEALNEKTPSLAKAIAAWKLQLEDDYAFQEFNWKADVVEAWIADKETSLKTNGNGADLGDFLTLLAKQDTLDASLQSFQQERLPEITDLKDKLISAQHNQSKAIEERYAALLKRWEQLLEASAVHRQKLLEKQLPLQKAEDLFVEFAHKASALNNWCEKMEENLSEPVHCVSLNEIRQLQKDHEDFLASLARAQADFKCLLELDQQIKALGVPSSPYTWLTVEVLERTWKHLSDIIEEREQELQKEEARQVKNFEMCQEFEQNASTFLQWILETRAYFLDGSLLKETGTLESQLEANKRKQKEIQAMKRQLTKIVDLGDNLEDALILDIKYSTIGLAQQWDQLYQLGLRMQHNLEQQIQAKDIKGVSEETLKEFSTIYKHFDENLTGRLTHKEFRSCLRGLNYYLPMVEEDEHEPKFEKFLDAVDPGRKGYVSLEDYTAFLIDKESENIKSSDEIENAFQALAEGKSYITKEDMKQALTPEQVSFCATHMQQYMDPRGRSHLSGYDYVGFTNSYFGN
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
100
Pig
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P02549 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S37 | Phosphorylation | Uniprot | |
| K59 | Acetylation | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| K283 | Acetylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| Y370 | Phosphorylation | Uniprot | |
| Y422 | Phosphorylation | Uniprot | |
| S507 | Phosphorylation | Uniprot | |
| S510 | Phosphorylation | Uniprot | |
| Y548 | Phosphorylation | Uniprot | |
| Y588 | Phosphorylation | Uniprot | |
| T635 | Phosphorylation | Uniprot | |
| T812 | Phosphorylation | Uniprot | |
| S818 | Phosphorylation | Uniprot | |
| S844 | O-Glycosylation | Uniprot | |
| Y986 | Phosphorylation | Uniprot | |
| S992 | Phosphorylation | Uniprot | |
| Y1064 | Phosphorylation | Uniprot | |
| S1167 | Phosphorylation | Uniprot | |
| S1250 | O-Glycosylation | Uniprot | |
| T1257 | Phosphorylation | Uniprot | |
| S1284 | Phosphorylation | Uniprot | |
| S1363 | Phosphorylation | Uniprot | |
| S1424 | Phosphorylation | Uniprot | |
| S1425 | Phosphorylation | Uniprot | |
| S1428 | Phosphorylation | Uniprot | |
| K1486 | Ubiquitination | Uniprot | |
| Y1499 | Phosphorylation | Uniprot | |
| Y1538 | Phosphorylation | Uniprot | |
| K1579 | Acetylation | Uniprot | |
| K1586 | Acetylation | Uniprot | |
| S1738 | O-Glycosylation | Uniprot | |
| K1803 | Ubiquitination | Uniprot | |
| S1901 | Phosphorylation | Uniprot | |
| T1910 | Phosphorylation | Uniprot | |
| S1912 | Phosphorylation | Uniprot | |
| S1976 | Phosphorylation | Uniprot | |
| K2051 | Acetylation | Uniprot | |
| K2200 | Acetylation | Uniprot | |
| S2269 | Phosphorylation | Uniprot | |
| T2288 | Phosphorylation | Uniprot | |
| Y2333 | Phosphorylation | Uniprot | |
| S2335 | Phosphorylation | Uniprot | |
| S2405 | Phosphorylation | Uniprot | |
| Y2407 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane.
Subcellular Location:
Cytoplasm>Cytoskeleton. Cytoplasm>Cell cortex.
Subunit Structure:
Composed of non-homologous chains, alpha and beta, which aggregate side-to-side in an antiparallel fashion to form dimers, tetramers, and higher polymers. Interacts with FASLG.
Family&Domains:
Belongs to the spectrin family.
Research Fields
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
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