AKR1B10 Antibody - #DF13405

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Product: AKR1B10 Antibody
Catalog: DF13405
Description: Rabbit polyclonal antibody to AKR1B10
Application: WB IHC
Reactivity: Human
Mol.Wt.: 36kDa; 36kD(Calculated).
Uniprot: O60218
RRID: AB_2846424

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
AKR1B10 Antibody detects endogenous levels of total AKR1B10.
RRID:
AB_2846424
Cite Format: Affinity Biosciences Cat# DF13405, RRID:AB_2846424.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AK1BA_HUMAN; AKR1B10; AKR1B11; AKR1B12; Aldo keto reductase family 1 member B10; aldo keto reductase family 1 member B11; Aldo-keto reductase family 1 member B10 (aldose reductase); Aldo-keto reductase family 1 member B10; Aldo-keto reductase family 1 member B11 (aldose reductase-like); aldose reductase like 1; Aldose reductase like; aldose reductase like peptide; Aldose reductase related protein; Aldose reductase-like; Aldose reductase-related protein; ALDRLn; ARL 1; ARL-1; ARL1; ARL1 Human small intestine reductase; ARP; hARP; HIS; HSI; SI reductase; Small intestine reductase;

Immunogens

Immunogen:
Uniprot:

O60218(AK1BA_HUMAN) >>Visit HPA database.

Gene(ID):
AKR1B10(57016)
Expression:
O60218 AK1BA_HUMAN:

Found in many tissues. Highly expressed in small intestine, colon and adrenal gland.

Sequence:
MATFVELSTKAKMPIVGLGTWKSPLGKVKEAVKVAIDAGYRHIDCAYVYQNEHEVGEAIQEKIQEKAVKREDLFIVSKLWPTFFERPLVRKAFEKTLKDLKLSYLDVYLIHWPQGFKSGDDLFPKDDKGNAIGGKATFLDAWEAMEELVDEGLVKALGVSNFSHFQIEKLLNKPGLKYKPVTNQVECHPYLTQEKLIQYCHSKGITVTAYSPLGSPDRPWAKPEDPSLLEDPKIKEIAAKHKKTAAQVLIRFHIQRNVIVIPKSVTPARIVENIQVFDFKLSDEEMATILSFNRNWRACNVLQSSHLEDYPFNAEY

PTMs - O60218 As Substrate

Site PTM Type Enzyme
S23 Phosphorylation
K27 Acetylation
Y40 Phosphorylation
Y47 Phosphorylation
Y49 Phosphorylation
K69 Acetylation
R70 Methylation
K95 Acetylation
K125 Acetylation
T137 Phosphorylation
K173 Ubiquitination
K177 Ubiquitination
K179 Acetylation
Y190 Phosphorylation
Y199 Phosphorylation
S215 Phosphorylation
K263 Acetylation
S282 Phosphorylation
T288 Phosphorylation
S291 Phosphorylation
Y310 Phosphorylation
Y316 Phosphorylation

Research Backgrounds

Function:

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays strong enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. Plays a critical role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls). Displays no reductase activity towards glucose.

Subcellular Location:

Lysosome. Secreted.
Note: Secreted through a lysosome-mediated non-classical pathway.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found in many tissues. Highly expressed in small intestine, colon and adrenal gland.

Family&Domains:

Belongs to the aldo/keto reductase family.

Research Fields

· Metabolism > Carbohydrate metabolism > Pentose and glucuronate interconversions.

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Metabolism > Carbohydrate metabolism > Galactose metabolism.

· Metabolism > Lipid metabolism > Glycerolipid metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Folate biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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