Slingshot homolog 1 Antibody - #DF13402

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Product: Slingshot homolog 1 Antibody
Catalog: DF13402
Description: Rabbit polyclonal antibody to Slingshot homolog 1
Application: WB IHC
Reactivity: Human
Prediction: Bovine, Horse, Sheep, Dog
Mol.Wt.: 115kDa; 116kD(Calculated).
Uniprot: Q8WYL5
RRID: AB_2846421

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Bovine(86%), Horse(100%), Sheep(88%), Dog(83%)
Clonality:
Polyclonal
Specificity:
Slingshot homolog 1 Antibody detects endogenous levels of total Slingshot homolog 1.
RRID:
AB_2846421
Cite Format: Affinity Biosciences Cat# DF13402, RRID:AB_2846421.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AW551225; Coro1c; coronin, actin binding protein 1C; Gm1394; Gm1395; hSSH-1L; mSSH 1L; OTTMUSP00000031415; Protein phosphatase Slingshot homolog 1; Slingshot 1; slingshot 1L; slingshot homolog 1; slingshot like 1; Slingshot1; SSH 1; SSH 1L; SSH-1L; SSH-like protein 1; Ssh1; SSH1_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q8WYL5(SSH1_HUMAN) >>Visit HPA database.

Gene(ID):
SSH1(54434)
Sequence:
MALVTLQRSPTPSAASSSASNSELEAGSEEDRKLNLSLSESFFMVKGAALFLQQGSSPQGQRSLQHPHKHAGDLPQHLQVMINLLRCEDRIKLAVRLESAWADRVRYMVVVYSSGRQDTEENILLGVDFSSKESKSCTIGMVLRLWSDTKIHLDGDGGFSVSTAGRMHIFKPVSVQAMWSALQVLHKACEVARRHNYFPGGVALIWATYYESCISSEQSCINEWNAMQDLESTRPDSPALFVDKPTEGERTERLIKAKLRSIMMSQDLENVTSKEIRNELEKQMNCNLKELKEFIDNEMLLILGQMDKPSLIFDHLYLGSEWNASNLEELQGSGVDYILNVTREIDNFFPGLFAYHNIRVYDEETTDLLAHWNEAYHFINKAKRNHSKCLVHCKMGVSRSASTVIAYAMKEFGWPLEKAYNYVKQKRSITRPNAGFMRQLSEYEGILDASKQRHNKLWRQQTDSSLQQPVDDPAGPGDFLPETPDGTPESQLPFLDDAAQPGLGPPLPCCFRRLSDPLLPSPEDETGSLVHLEDPEREALLEEAAPPAEVHRPARQPQQGSGLCEKDVKKKLEFGSPKGRSGSLLQVEETEREEGLGAGRWGQLPTQLDQNLLNSENLNNNSKRSCPNGMEDDAIFGILNKVKPSYKSCADCMYPTASGAPEASRERCEDPNAPAICTQPAFLPHITSSPVAHLASRSRVPEKPASGPTEPPPFLPPAGSRRADTSGPGAGAALEPPASLLEPSRETPKVLPKSLLLKNSHCDKNPPSTEVVIKEESSPKKDMKPAKDLRLLFSNESEKPTTNSYLMQHQESIIQLQKAGLVRKHTKELERLKSVPADPAPPSRDGPASRLEASIPEESQDPAALHELGPLVMPSQAGSDEKSEAAPASLEGGSLKSPPPFFYRLDHTSSFSKDFLKTICYTPTSSSMSSNLTRSSSSDSIHSVRGKPGLVKQRTQEIETRLRLAGLTVSSPLKRSHSLAKLGSLTFSTEDLSSEADPSTVADSQDTTLSESSFLHEPQGTPRDPAATSKPSGKPAPENLKSPSWMSKS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Sheep
88
Bovine
86
Dog
83
Chicken
50
Pig
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WYL5 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T5 Phosphorylation
S9 Phosphorylation
T11 Phosphorylation
S37 Phosphorylation
S57 Phosphorylation
S261 Phosphorylation
S400 Phosphorylation
S402 Phosphorylation Q15139 (PRKD1)
T403 Phosphorylation
Y407 Phosphorylation
S428 Phosphorylation
T430 Phosphorylation
S441 Phosphorylation
Y443 Phosphorylation
K456 Ubiquitination
T462 Phosphorylation
S464 Phosphorylation
S465 Phosphorylation
S515 Phosphorylation
S521 Phosphorylation
T526 Phosphorylation
S576 Phosphorylation
S583 Phosphorylation
T590 Phosphorylation
S645 Phosphorylation
Y646 Phosphorylation
K758 Methylation
S812 Phosphorylation
T826 Phosphorylation P31749 (AKT1)
S834 Phosphorylation
S854 Phosphorylation
S889 Phosphorylation
S894 Phosphorylation
S897 Phosphorylation
T908 Phosphorylation
S910 Phosphorylation
T922 Phosphorylation
T933 Phosphorylation
S935 Phosphorylation
S936 Phosphorylation
S937 Phosphorylation Q9BZL6 (PRKD2) , Q15139 (PRKD1) , Q13557 (CAMK2D)
S938 Phosphorylation
S940 Phosphorylation
S943 Phosphorylation
K952 Acetylation
S970 Phosphorylation
S971 Phosphorylation
S978 Phosphorylation Q13557 (CAMK2D) , Q15139 (PRKD1) , Q9BZL6 (PRKD2)
T1021 Phosphorylation
K1041 Methylation
S1042 Phosphorylation

Research Backgrounds

Function:

Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.

PTMs:

Phosphorylated. Inhibitory phosphorylation by PAK4 promotes binding to YWHAZ. Phosphorylation at Ser-978 is decreased by stimuli which promote actin reorganization and lamellipodia formation. Can be dephosphorylated and activated by PPP3CA/calcineurin A. Phosphorylation decreases immediately prior to telophase.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell projection>Lamellipodium. Cleavage furrow. Midbody.
Note: Also recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control of actin dynamics at sites of cell locomotion. Also localized to the cleavage furrow and the midbody during cytokinesis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with actin and this stimulates phosphatase activity. Also interacts with LIMK1 and with the 14-3-3 proteins YWHAB, YWHAG, YWHAQ, and YWHAZ. Interaction with 14-3-3 proteins inhibits phosphatase activity and also blocks recruitment to lamellipodia and stimulation by actin.

Family&Domains:

Belongs to the protein-tyrosine phosphatase family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

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