FosB Antibody - #AF5010

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Product: FosB Antibody
Catalog: AF5010
Description: Rabbit polyclonal antibody to FosB
Application: WB IF/ICC
Reactivity: Human, Mouse, Monkey
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 35kDa; 36kD(Calculated).
Uniprot: P53539
RRID: AB_2834932

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
FosB Antibody detects endogenous levels of total FosB.
RRID:
AB_2834932
Cite Format: Affinity Biosciences Cat# AF5010, RRID:AB_2834932.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Activator protein 1; AP 1; DKFZp686C0818; FBJ murine osteosarcoma viral oncogene homolog B; fosB; FOSB_HUMAN; G0/G1 switch regulatory protein 3; G0S3; GOS 3; GOS B; GOS3; GOSB; MGC42291; Oncogene FOS B; Oncogene FOSB; Protein fos B; Protein fosB;

Immunogens

Immunogen:
Uniprot:

P53539(FOSB_HUMAN) >>Visit HPA database.

Gene(ID):
FOSB(2354)
Description:
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1.
Sequence:
MFQAFPGDYDSGSRCSSSPSAESQYLSSVDSFGSPPTAAASQECAGLGEMPGSFVPTVTAITTSQDLQWLVQPTLISSMAQSQGQPLASQPPVVDPYDMPGTSYSTPGMSGYSSGGASGSGGPSTSGTTSGPGPARPARARPRRPREETLTPEEEEKRRVRRERNKLAAAKCRNRRRELTDRLQAETDQLEEEKAELESEIAELQKEKERLEFVLVAHKPGCKIPYEEGPGPGPLAEVRDLPGSAPAKEDGFSWLLPPPPPPPLPFQTSQDAPPNLTASLFTHSEVQVLGDPFPVVNPSYTSSFVLTCPEVSAFAGAQRTSGSDQPSDPLNSPSLLAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Zebrafish
89
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P53539 As Substrate

Site PTM Type Enzyme
Y9 Phosphorylation
S11 Phosphorylation
S13 Phosphorylation
S27 Phosphorylation P68400 (CSNK2A1)
T151 Phosphorylation

Research Backgrounds

Function:

FosB interacts with Jun proteins enhancing their DNA binding activity.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer.

Family&Domains:

Belongs to the bZIP family. Fos subfamily.

Research Fields

· Human Diseases > Substance dependence > Cocaine addiction.

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

References

1). MAPK/AP-1 pathway regulates benzidine-induced cell proliferation through the control of cell cycle in human normal bladder epithelial cells. Oncology Letters, 2018 (PubMed: 30197677) [IF=2.9]

Application: WB    Species: human    Sample: SV‑HUC‑1

Figure 4. |Effects of benzidine on the expression and activation of mitogen‑activated protein kinases and activating protein‑1 monomers. (E) The Fos family, including p‑c‑Fos, FosB and Fra‑1, were all upregulated.

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