Ankyrin erythroid Antibody - #DF13399

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Product: Ankyrin erythroid Antibody
Catalog: DF13399
Description: Rabbit polyclonal antibody to Ankyrin erythroid
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 206kDa; 206kD(Calculated).
Uniprot: P16157
RRID: AB_2846418

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Ankyrin erythroid Antibody detects endogenous levels of total Ankyrin erythroid.
RRID:
AB_2846418
Cite Format: Affinity Biosciences Cat# DF13399, RRID:AB_2846418.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ANK; ANK-1; Ank1; ANK1_HUMAN; Ankyrin 1; Ankyrin 1, erythrocytic; Ankyrin R; Ankyrin-1; Ankyrin-R; Erythrocyte ankyrin; SPH1; SPH2;

Immunogens

Immunogen:
Uniprot:

P16157(ANK1_HUMAN) >>Visit HPA database.

Gene(ID):
ANK1(286)
Expression:
P16157 ANK1_HUMAN:

Isoform Mu17, isoform Mu18, isoform Mu19 and isoform Mu20 are expressed in skeletal muscle. Isoform Br21 is expressed in brain.

Sequence:
MPYSVGFREADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVLKVVTDETSFVLVSDKHRMSFPETVDEILDVSEDEGEELISFKAERRDSRDVDEEKELLDFVPKLDQVVESPAIPRIPCAMPETVVIRSEEQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFLVSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLSTPPPLAEEEGLASRIIALGPTGAQFLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHRSRYGESYLDQILNGMDEELGSLEELEKKRVCRIITTDFPLYFVIMSRLCQDYDTIGPEGGSLKSKLVPLVQATFPENAVTKRVKLALQAQPVPDELVTKLLGNQATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKLVYANECANFTTNVSARFWLSDCPRTAEAVNFATLLYKELTAVPYMAKFVIFAKMNDPREGRLRCYCMTDDKVDKTLEQHENFVEVARSRDIEVLEGMSLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAMPVKVRDSSREPGGSLSFLRKAMKYEDTQHILCHLNITMPPCAKGSGAEDRRRTPTPLALRYSILSESTPGSLSGTEQAEMKMAVISEHLGLSWAELARELQFSVEDINRIRVENPNSLLEQSVALLNLWVIREGQNANMENLYTALQSIDRGEIVNMLEGSGRQSRNLKPDRRHTDRDYSLSPSQMNGYSSLQDELLSPASLGCALSSPLRADQYWNEVAVLDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDATGHEWKLEGALSEEPRGPELGSLELVEDDTVDSDATNGLIDLLEQEEGQRSEEKLPGSKRQDDATGAGQDSENEVSLVSGHQRGQARITHSPTVSQVTERSQDRLQDWDADGSIVSYLQDAAQGSWQEEVTQGPHSFQGTSTMTEGLEPGGSQEYEKVLVSVSEHTWTEQPEAESSQADRDRRQQGQEEQVQEAKNTFTQVVQGNEFQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQIDLSSADAAQEHEEVTVEGPLEDPSELEVDIDYFMKHSKDHTSTPNP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Rabbit
100
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16157 As Substrate

Site PTM Type Enzyme
Y3 Phosphorylation
S4 Phosphorylation
T14 Phosphorylation
S15 Phosphorylation
Y100 Phosphorylation
T208 Phosphorylation
Y216 Phosphorylation
S231 Phosphorylation
T235 Phosphorylation
S288 O-Glycosylation
S366 Phosphorylation
K380 Acetylation
S429 Phosphorylation
K436 Ubiquitination
K454 Ubiquitination
T500 Phosphorylation
K534 Ubiquitination
Y546 Phosphorylation
S594 Phosphorylation
S597 Phosphorylation
Y603 Phosphorylation
T604 Phosphorylation
Y624 Phosphorylation
Y702 Phosphorylation
T703 Phosphorylation
Y711 Phosphorylation
Y735 Phosphorylation
S736 Phosphorylation
S759 Phosphorylation
S781 Phosphorylation
S794 O-Glycosylation
S817 Phosphorylation
S826 Phosphorylation
S834 Phosphorylation
S856 Phosphorylation
Y884 Phosphorylation
S907 Phosphorylation
S960 O-Glycosylation
T961 Phosphorylation
Y1073 Phosphorylation
K1102 Acetylation
S1162 O-Glycosylation
T1227 Phosphorylation
Y1230 Phosphorylation
Y1259 Phosphorylation
S1312 Phosphorylation
T1378 Phosphorylation
T1380 Phosphorylation
S1396 Phosphorylation
S1398 Phosphorylation
S1428 Phosphorylation
Y1468 Phosphorylation
S1486 Phosphorylation
S1523 Phosphorylation
S1533 Phosphorylation
S1593 Phosphorylation
S1607 Phosphorylation
S1666 Phosphorylation
S1671 Phosphorylation
T1684 Phosphorylation
S1686 Phosphorylation
T1688 Phosphorylation
S1690 Phosphorylation
S1696 Phosphorylation
Y1750 Phosphorylation

Research Backgrounds

Function:

Attaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions.

Together with obscurin in skeletal muscle may provide a molecular link between the sarcoplasmic reticulum and myofibrils.

PTMs:

Regulated by phosphorylation.

Palmitoylated.

Hydroxylated by HIF1AN at several asparagine and 1 aspartate residue within ANK repeat region. Hydroxylation seems to increase the conformational stability of this region and may also modulate protein-protein interactions mediated by the ANK repeat region.

Subcellular Location:

Cytoplasm>Cytoskeleton.
Note: Probably the other erythrocyte (Er) isoforms, are located near the surface of erythrocytic plasma membrane.

Membrane. Cytoplasm>Myofibril>Sarcomere>M line.
Note: Colocalizes with OBSCN isoform 3/obscurin at the M line in differentiated skeletal muscle cells.

Sarcoplasmic reticulum.

Sarcoplasmic reticulum.

Sarcoplasmic reticulum.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform Mu17, isoform Mu18, isoform Mu19 and isoform Mu20 are expressed in skeletal muscle. Isoform Br21 is expressed in brain.

Subunit Structure:

Interacts with a number of integral membrane proteins and cytoskeletal proteins. Interacts (via N-terminus) with SPTB/spectrin (beta chain). Interacts (via N-terminus ANK repeats) with SLC4A1/erythrocyte membrane protein band 3 (via cytoplasmic N-terminus). Also interacts with TTN/titin. Isoform Mu17 interacts with OBSCN isoform 3/obscurin. Interacts with HIF1AN.

Family&Domains:

The 55 kDa regulatory domain is involved in regulating binding of SPTB/spectrin (beta chain) and SLC4A1/erythrocyte membrane protein band 3.

The ANK repeat region forms a spiral around a large central cavity and is involved in binding of ion transporters.

The tandem configuration of the two ZU5 and the UPA domains forms a structural supramodule termed ZZU. ZU5-1 mediates interaction with beta-spectrin, and the ZU5-1/UPA interface is required for ankyrin's function other than binding to spectrin (By similarity).

Research Fields

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

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