EBF2 Antibody - #DF13398

Figure 2. MiR-19b-3p inhibits osteoblast differentiation. (A) RT-PCR analysis was used 443 to detect miRNA-19b-3p expression levels in BMSC-derived osteoblasts after 444 miR-19b-3p-overexpressed or knockdown by corresponding lentivirus. (B) ALP activity in 445 BMSC-derived osteoblasts after miRNA-19b-3p overexpression or knockdown by 446 corresponding lentivirus. (C) RT-PCR was used to detect EBF2 mRNA expression levels in BMSC-derived osteoblasts after miRNA-19b-3p overexpression or knockdown by 448 corresponding lentivirus. Western blot assay was used to detect (D and E) EBF2, (F-H) 449 RANKL and OPG protein expression levels in BMSC-derived osteoblasts after 450 miRNA-19b-3p overexpression or knockdown by corresponding lentivirus. (I) The ratio of 451 OPG to RANKL in BMSC-derived osteoblasts after miRNA-19b-3p overexpression or 452 knockdown by corresponding lentivirus (J) Alizarin red staining was used to detect cell 453 mineralization at day 21 after differentiation after miRNA-19b-3p overexpression or 454 knockdown at 100×. Data are analyzed using unpaired Student’s t-tests and presented as the 455 mean ± SD from three independent experiments. * p < 0.05; ** p < 0.01 vs. the indicated 456 group.