Acid sphingomyelinase Antibody - #DF13384

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Product: Acid sphingomyelinase Antibody
Catalog: DF13384
Description: Rabbit polyclonal antibody to Acid sphingomyelinase
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 69kDa, 55 kDa; 70kD(Calculated).
Uniprot: P17405
RRID: AB_2846403

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(86%)
Clonality:
Polyclonal
Specificity:
Acid sphingomyelinase Antibody detects endogenous levels of total Acid sphingomyelinase.
RRID:
AB_2846403
Cite Format: Affinity Biosciences Cat# DF13384, RRID:AB_2846403.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Acid sphingomyelinase; ASM; ASM_HUMAN; aSMase; NPD; Smpd1; Sphingomyelin phosphodiesterase 1 acid lysosomal; Sphingomyelin phosphodiesterase;

Immunogens

Immunogen:
Uniprot:

P17405(ASM_HUMAN) >>Visit HPA database.

Gene(ID):
SMPD1(6609)
Sequence:
MPRYGASLRQSCPRSGREQGQDGTAGAPGLLWMGLVLALALALALALALSDSRVLWAPAEAHPLSPQGHPARLHRIVPRLRDVFGWGNLTCPICKGLFTAINLGLKKEPNVARVGSVAIKLCNLLKIAPPAVCQSIVHLFEDDMVEVWRRSVLSPSEACGLLLGSTCGHWDIFSSWNISLPTVPKPPPKPPSPPAPGAPVSRILFLTDLHWDHDYLEGTDPDCADPLCCRRGSGLPPASRPGAGYWGEYSKCDLPLRTLESLLSGLGPAGPFDMVYWTGDIPAHDVWHQTRQDQLRALTTVTALVRKFLGPVPVYPAVGNHESTPVNSFPPPFIEGNHSSRWLYEAMAKAWEPWLPAEALRTLRIGGFYALSPYPGLRLISLNMNFCSRENFWLLINSTDPAGQLQWLVGELQAAEDRGDKVHIIGHIPPGHCLKSWSWNYYRIVARYENTLAAQFFGHTHVDEFEVFYDEETLSRPLAVAFLAPSATTYIGLNPGYRVYQIDGNYSGSSHVVLDHETYILNLTQANIPGAIPHWQLLYRARETYGLPNTLPTAWHNLVYRMRGDMQLFQTFWFLYHKGHPPSEPCGTPCRLATLCAQLSARADSPALCRHLMPDGSLPEAQSLWPRPLFC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
86
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P17405 As Substrate

Site PTM Type Enzyme
S7 Phosphorylation
N88 N-Glycosylation
N177 N-Glycosylation
S233 Phosphorylation
S239 Phosphorylation
N337 N-Glycosylation
N397 N-Glycosylation
N505 N-Glycosylation
S510 Phosphorylation
N522 N-Glycosylation

Research Backgrounds

Function:

Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol.

Isoform 2 lacks residues that bind the cofactor Zn(2+) and has no enzyme activity.

Isoform 3 lacks residues that bind the cofactor Zn(2+) and has no enzyme activity.

Subcellular Location:

Lysosome. Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Monomer.

Family&Domains:

Belongs to the acid sphingomyelinase family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Metabolism > Lipid metabolism > Sphingolipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Nitric oxide induces HepG2 cell death via extracellular signal-regulated protein kinase activation by regulating acid sphingomyelinase. MOLECULAR BIOLOGY REPORTS (PubMed: 33025504) [IF=2.8]

Application: WB    Species: human    Sample: HepG2 cells

Fig. 2| mRNA and protein levels of acid sphingomyelinase(ASM) in diethylenetriamine/nitric oxide (DETA–NO)-treated HepG2 cells. a mRNA level of ASM in HepG2 cells treated with diferent concentrations of DETA–NO were assayed via real-time polymerase chain reaction. b Protein levels of ASM in HepG2 cells treated with diferent concentrations of DETA–NO were detected via western blotting. *P<0.05, **P<0.01,***P<0.001, compared with untreated HepG2 cells

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