EST1A Antibody - #DF13372

(3)  
Product: EST1A Antibody
Catalog: DF13372
Description: Rabbit polyclonal antibody to EST1A
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 160kDa; 160kD(Calculated).
Uniprot: Q86US8
RRID: AB_2846391

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
EST1A Antibody detects endogenous levels of total EST1A.
RRID:
AB_2846391
Cite Format: Affinity Biosciences Cat# DF13372, RRID:AB_2846391.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C17orf31; EST1-like protein A; Ever shorter telomeres 1A; hSmg5/7a; KIAA0732; Smg-6 homolog; SMG6; Telomerase subunit EST1A; Telomerase-binding protein EST1A;

Immunogens

Immunogen:
Uniprot:

Q86US8(EST1A_HUMAN) >>Visit HPA database.

Gene(ID):
SMG6(23293)
Expression:
Q86US8 EST1A_HUMAN:

Ubiquitous.

Sequence:
MAEGLERVRISASELRGILATLAPQAGSRENMKELKEARPRKDNRRPDLEIYKPGLSRLRNKPKIKEPPGSEEFKDEIVNDRDCSAVENGTQPVKDVCKELNNQEQNGPIDPENNRGQESFPRTAGQEDRSLKIIKRTKKPDLQIYQPGRRLQTVSKESASRVEEEEVLNQVEQLRVEEDECRGNVAKEEVANKPDRAEIEKSPGGGRVGAAKGEKGKRMGKGEGVRETHDDPARGRPGSAKRYSRSDKRRNRYRTRSTSSAGSNNSAEGAGLTDNGCRRRRQDRTKERPRLKKQVSVSSTDSLDEDRIDEPDGLGPRRSSERKRHLERNWSGRGEGEQKNSAKEYRGTLRVTFDAEAMNKESPMVRSARDDMDRGKPDKGLSSGGKGSEKQESKNPKQELRGRGRGILILPAHTTLSVNSAGSPESAPLGPRLLFGSGSKGSRSWGRGGTTRRLWDPNNPDQKPALKTQTPQLHFLDTDDEVSPTSWGDSRQAQASYYKFQNSDNPYYYPRTPGPASQYPYTGYNPLQYPVGPTNGVYPGPYYPGYPTPSGQYVCSPLPTSTMSPEEVEQHMRNLQQQELHRLLRVADNQELQLSNLLSRDRISPEGLEKMAQLRAELLQLYERCILLDIEFSDNQNVDQILWKNAFYQVIEKFRQLVKDPNVENPEQIRNRLLELLDEGSDFFDSLLQKLQVTYKFKLEDYMDGLAIRSKPLRKTVKYALISAQRCMICQGDIARYREQASDTANYGKARSWYLKAQHIAPKNGRPYNQLALLAVYTRRKLDAVYYYMRSLAASNPILTAKESLMSLFEETKRKAEQMEKKQHEEFDLSPDQWRKGKKSTFRHVGDDTTRLEIWIHPSHPRSSQGTESGKDSEQENGLGSLSPSDLNKRFILSFLHAHGKLFTRIGMETFPAVAEKVLKEFQVLLQHSPSPIGSTRMLQLMTINMFAVHNSQLKDCFSEECRSVIQEQAAALGLAMFSLLVRRCTCLLKESAKAQLSSPEDQDDQDDIKVSSFVPDLKELLPSVKVWSDWMLGYPDTWNPPPTSLDLPSHVAVDVWSTLADFCNILTAVNQSEVPLYKDPDDDLTLLILEEDRLLSGFVPLLAAPQDPCYVEKTSDKVIAADCKRVTVLKYFLEALCGQEEPLLAFKGGKYVSVAPVPDTMGKEMGSQEGTRLEDEEEDVVIEDFEEDSEAEGSGGEDDIRELRAKKLALARKIAEQQRRQEKIQAVLEDHSQMRQMELEIRPLFLVPDTNGFIDHLASLARLLESRKYILVVPLIVINELDGLAKGQETDHRAGGYARVVQEKARKSIEFLEQRFESRDSCLRALTSRGNELESIAFRSEDITGQLGNNDDLILSCCLHYCKDKAKDFMPASKEEPIRLLREVVLLTDDRNLRVKALTRNVPVRDIPAFLTWAQVG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
57
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86US8 As Substrate

Site PTM Type Enzyme
S11 Phosphorylation
S28 Phosphorylation
Y52 Phosphorylation
S57 Phosphorylation
S85 Phosphorylation
T91 Phosphorylation
T138 Phosphorylation
Y146 Phosphorylation
S203 Phosphorylation
S240 Phosphorylation
S245 Phosphorylation
S247 Phosphorylation
T256 Phosphorylation
S260 Phosphorylation
S264 Phosphorylation
S267 Phosphorylation
S297 Phosphorylation
S332 Phosphorylation
T353 Phosphorylation
S363 Phosphorylation
R404 Methylation
R406 Methylation
S424 Phosphorylation
S438 Phosphorylation
S440 Phosphorylation
K441 Ubiquitination
S443 Phosphorylation
T471 Phosphorylation
T479 Phosphorylation
S484 Phosphorylation
T486 Phosphorylation
S487 Phosphorylation
Y508 Phosphorylation
Y509 Phosphorylation
R512 Methylation
Y769 Phosphorylation
Y778 Phosphorylation
Y788 Phosphorylation
Y789 Phosphorylation
S796 Phosphorylation
K803 Acetylation
K814 Ubiquitination
K823 Ubiquitination
S831 Phosphorylation
S864 Phosphorylation
S865 Phosphorylation
T868 Phosphorylation
S870 Phosphorylation
S874 Phosphorylation
S884 Phosphorylation
K918 Acetylation
K995 Ubiquitination
S1000 Phosphorylation
K1011 Ubiquitination
K1149 Ubiquitination
K1225 Ubiquitination
K1309 Ubiquitination
K1376 Ubiquitination
T1390 Phosphorylation

Research Backgrounds

Function:

Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. May have a general role in telomere regulation. Promotes in vitro the ability of TERT to elongate telomeres. Overexpression induces telomere uncapping, chromosomal end-to-end fusions (telomeric DNA persists at the fusion points) and did not perturb TRF2 telomeric localization. Binds to the single-stranded 5'-(GTGTGG)(4)GTGT-3' telomeric DNA, but not to a telomerase RNA template component (TER).

Plays a role in nonsense-mediated mRNA decay. Is thought to provide a link to the mRNA degradation machinery as it has endonuclease activity required to initiate NMD, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Degrades single-stranded RNA (ssRNA), but not ssDNA or dsRNA.

Subcellular Location:

Nucleus>Nucleolus. Chromosome>Telomere. Cytoplasm>Cytosol.
Note: Particularly enriched in the nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

May form homooligomers. Associated component of the telomerase holoenzyme complex. Interacts with TERT, independently of the telomerase RNA. Interacts with SMG1, SMG5, SMG7, UPF1, UPF2, UPF3B and the PP2A catalytic subunits. Also interacts with the exon junction complex (EJC) composed at least of CASC3, EIF4A3, MAGOH and RBM8A; required for the process of nonsense-mediated mRNA decay.

Family&Domains:

The PINc domain confers endonuclease activity and is expected to bind the catalytic metal ion.

Research Fields

· Genetic Information Processing > Translation > mRNA surveillance pathway.

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