XLF Antibody | Affinity Biosciences

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Western blot analysis of extracts from Hela cells, using XLF Antibody.

Product:	XLF Antibody
Catalog:	DF13366
Description:	Rabbit polyclonal antibody to XLF
Application:	WB
Reactivity:	Human
Prediction:	Xenopus
Mol.Wt.:	33kDa; 33kD(Calculated).
Uniprot:	Q9H9Q4
RRID:	AB_2846385

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Xenopus(83%)

Clonality:

Polyclonal

Specificity:

XLF Antibody detects endogenous levels of total XLF.

RRID:

AB_2846385
Cite Format: Affinity Biosciences Cat# DF13366, RRID:AB_2846385.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Cernunnos; FLJ12610; NHEJ 1; Nhej1; NHEJ1, S. cerevisiae, homolog of; NHEJ1_HUMAN; Non homologous end joining factor 1; Non-homologous end-joining factor 1; Nonhomologous end joining factor 1; OTTHUMP00000164168; OTTHUMP00000206275; OTTHUMP00000206279; Protein cernunnos; XLF; XRCC4 like factor; XRCC4-like factor;

Immunogens

Immunogen:

Uniprot:

Q9H9Q4(NHEJ1_HUMAN) >>Visit HPA database.

Gene(ID):

NHEJ1(79840)

Expression:

Q9H9Q4 NHEJ1_HUMAN:

Ubiquitously expressed.

Sequence:

Q9H9Q4 NHEJ1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEELEQGLLMQPWAWLQLAENSLLAKVFITKQGYALLVSDLQQVWHEQVDTSVVSQRAKELNKRLTAPPAAFLCHLDNLLRPLLKDAAHPSEATFSCDCVADALILRVRSELSGLPFYWNFHCMLASPSLVSQHLIRPLMGMSLALQCQVRELATLLHMKDLEIQDYQESGATLIRDRLKTEPFEENSFLEQFMIEKLPEACSIGDGKPFVMNLQDLYMAVTTQEVQVGQKHQGAGDPHTSNSASLQGIDSQCVNQPEQLVSSAPTLSAPEKESTGTSGPLQRPQLSKVKRKKPRGLFS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Xenopus

Pig

Horse

Bovine

Sheep

Dog

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H9Q4 As Substrate

Q9H9Q4

Site	PTM Type	Enzyme	Source
S55	Phosphorylation	P78527 (PRKDC)	Uniprot
S132	Phosphorylation	P78527 (PRKDC)	Uniprot
K180	Ubiquitination		Uniprot
T181	Phosphorylation	P31749 (AKT1)	Uniprot
S203	Phosphorylation		Uniprot
T223	Phosphorylation	P78527 (PRKDC)	Uniprot
S245	Phosphorylation	P78527 (PRKDC)	Uniprot
S251	Phosphorylation	Q13315 (ATM) , P78527 (PRKDC)	Uniprot
S262	Phosphorylation		Uniprot
S263	Phosphorylation	P78527 (PRKDC)	Uniprot
T266	Phosphorylation	P78527 (PRKDC)	Uniprot
S268	Phosphorylation		Uniprot
S287	Phosphorylation		Uniprot
K288	Methylation		Uniprot

Research Backgrounds

Q9H9Q4_NHEJ1_HUMAN

Function:

DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary. Binds DNA in a length-dependent manner.

Subcellular Location:

Nucleus.

Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Exists mainly as a homodimer. Associates with the non-homologous end joining (NHEJ) complex which is at least composed of the core proteins XRCC5/Ku80, XRCC6/Ku70, PRKDC/DNA-PKcs, LIG4 and XRCC4.

Family&Domains:

The coiled-coil region mediates homodimerization.

Belongs to the XLF family.

Research Fields

· Genetic Information Processing > Replication and repair > Non-homologous end-joining.

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XLF Antibody - #DF13366