CRM1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Hela cells, using CRM1 Antibody.

Product:	CRM1 Antibody
Catalog:	DF13364
Description:	Rabbit polyclonal antibody to CRM1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	123kDa; 123kD(Calculated).
Uniprot:	O14980
RRID:	AB_2846383

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

CRM1 Antibody detects endogenous levels of total CRM1.

RRID:

AB_2846383
Cite Format: Affinity Biosciences Cat# DF13364, RRID:AB_2846383.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Chromosome region maintenance 1 protein homolog; CRM 1; CRM1 homolog; DKFZp686B1823; emb; Exp 1; Exp1; Exportin 1; Exportin-1; Exportin1; XPO 1; xpo1; XPO1_HUMAN;

Immunogens

Immunogen:

Uniprot:

O14980(XPO1_HUMAN) >>Visit HPA database.

Gene(ID):

XPO1(7514)

Expression:

O14980 XPO1_HUMAN:

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney.

Sequence:

O14980 XPO1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPAIMTMLADHAARQLLDFSQKLDINLLDNVVNCLYHGEGAQQRMAQEVLTHLKEHPDAWTRVDTILEFSQNMNTKYYGLQILENVIKTRWKILPRNQCEGIKKYVVGLIIKTSSDPTCVEKEKVYIGKLNMILVQILKQEWPKHWPTFISDIVGASRTSESLCQNNMVILKLLSEEVFDFSSGQITQVKSKHLKDSMCNEFSQIFQLCQFVMENSQNAPLVHATLETLLRFLNWIPLGYIFETKLISTLIYKFLNVPMFRNVSLKCLTEIAGVSVSQYEEQFVTLFTLTMMQLKQMLPLNTNIRLAYSNGKDDEQNFIQNLSLFLCTFLKEHDQLIEKRLNLRETLMEALHYMLLVSEVEETEIFKICLEYWNHLAAELYRESPFSTSASPLLSGSQHFDVPPRRQLYLPMLFKVRLLMVSRMAKPEEVLVVENDQGEVVREFMKDTDSINLYKNMRETLVYLTHLDYVDTERIMTEKLHNQVNGTEWSWKNLNTLCWAIGSISGAMHEEDEKRFLVTVIKDLLGLCEQKRGKDNKAIIASNIMYIVGQYPRFLRAHWKFLKTVVNKLFEFMHETHDGVQDMACDTFIKIAQKCRRHFVQVQVGEVMPFIDEILNNINTIICDLQPQQVHTFYEAVGYMIGAQTDQTVQEHLIEKYMLLPNQVWDSIIQQATKNVDILKDPETVKQLGSILKTNVRACKAVGHPFVIQLGRIYLDMLNVYKCLSENISAAIQANGEMVTKQPLIRSMRTVKRETLKLISGWVSRSNDPQMVAENFVPPLLDAVLIDYQRNVPAAREPEVLSTMAIIVNKLGGHITAEIPQIFDAVFECTLNMINKDFEEYPEHRTNFFLLLQAVNSHCFPAFLAIPPTQFKLVLDSIIWAFKHTMRNVADTGLQILFTLLQNVAQEEAAAQSFYQTYFCDILQHIFSVVTDTSHTAGLTMHASILAYMFNLVEEGKISTSLNPGNPVNNQIFLQEYVANLLKSAFPHLQDAQVKLFVTGLFSLNQDIPAFKEHLRDFLVQIKEFAGEDTSDLFLEEREIALRQADEEKHKRQMSVPGIFNPHEIPEEMCD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14980 As Substrate

O14980

Site	PTM Type	Enzyme	Source
K22	Ubiquitination		Uniprot
K54	Ubiquitination		Uniprot
T65	Phosphorylation		Uniprot
K88	Ubiquitination		Uniprot
K92	Ubiquitination		Uniprot
K122	Ubiquitination		Uniprot
K124	Ubiquitination		Uniprot
K144	Acetylation		Uniprot
K144	Ubiquitination		Uniprot
C164	S-Nitrosylation		Uniprot
T187	Phosphorylation		Uniprot
K190	Ubiquitination		Uniprot
K192	Ubiquitination		Uniprot
Y252	Phosphorylation		Uniprot
S264	Phosphorylation		Uniprot
S384	Phosphorylation		Uniprot
S387	Phosphorylation		Uniprot
S389	Phosphorylation		Uniprot
S391	Phosphorylation	P06493 (CDK1)	Uniprot
S395	Phosphorylation		Uniprot
S397	Phosphorylation		Uniprot
Y409	Phosphorylation		Uniprot
K415	Acetylation		Uniprot
K415	Ubiquitination		Uniprot
K426	Ubiquitination		Uniprot
K446	Acetylation		Uniprot
K446	Ubiquitination		Uniprot
S450	Phosphorylation		Uniprot
K455	Acetylation		Uniprot
K455	Ubiquitination		Uniprot
Y469	Phosphorylation		Uniprot
K479	Ubiquitination		Uniprot
T519	Phosphorylation		Uniprot
C528	S-Nitrosylation		Uniprot
K531	Acetylation		Uniprot
K531	Ubiquitination		Uniprot
K537	Acetylation		Uniprot
K563	Ubiquitination		Uniprot
K568	Acetylation		Uniprot
K590	Ubiquitination		Uniprot
K674	Ubiquitination		Uniprot
K680	Ubiquitination		Uniprot
K686	Acetylation		Uniprot
K686	Ubiquitination		Uniprot
S690	Phosphorylation		Uniprot
K693	Acetylation		Uniprot
K693	Ubiquitination		Uniprot
T694	Phosphorylation		Uniprot
K700	Ubiquitination		Uniprot
K757	Acetylation		Uniprot
K757	Ubiquitination		Uniprot
S764	Phosphorylation		Uniprot
S802	Phosphorylation		Uniprot
T816	Phosphorylation		Uniprot
Y841	Phosphorylation		Uniprot
T1030	Phosphorylation		Uniprot
S1031	Phosphorylation		Uniprot
R1043	Methylation		Uniprot
S1055	Phosphorylation		Uniprot
C1070	S-Nitrosylation		Uniprot

Research Backgrounds

O14980_XPO1_HUMAN

Function:

Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap.

(Microbial infection) Mediates the export of unspliced or incompletely spliced RNAs out of the nucleus from different viruses including HIV-1, HTLV-1 and influenza A. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization.

Subcellular Location:

Cytoplasm. Nucleus>Nucleoplasm. Nucleus>Cajal body. Nucleus>Nucleolus.
Note: Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm.

Tissue Specificity:

Subunit Structure:

Found in a U snRNA export complex with PHAX/RNUXA, NCBP1/CBP80, NCBP2/CBP20, RAN, XPO1 and m7G-capped RNA (By similarity). Component of a nuclear export receptor complex composed of KPNB1, RAN, SNUPN and XPO1. Found in a trimeric export complex with SNUPN, RAN and XPO1. Found in a nuclear export complex with RANBP3 and RAN. Found in a 60S ribosomal subunit export complex with NMD3, RAN, XPO1. Interacts with DDX3X, NMD3, NUP42, NUP88, NUP214, RANBP3 and TERT. Interacts with NEMF (via its N-terminus). Interacts with the monomeric form of BIRC5/survivin deacetylated at 'Lys-129'. Interacts with DTNBP1 and SERTAD2; the interactions translocate DTNBP1 and SERTAD2 out of the nucleus. Interacts with ATF2. Interacts with SLC35G1 and STIM1. Interacts with DCAF8. Interacts with CPEB3. Interacts with HAX1. Interacts with BOK; translocates to the cytoplasm. Interacts with HSP90AB1.

(Microbial infection) Interacts with HIV-1 Rev.

(Microbial infection) Interacts with HTLV-1 Rex.

(Microbial infection) Interacts with influenza A nucleoprotein.

(Microbial infection) Interacts with Epstein-Barr virus protein BMLF1.

(Microbial infection) Part of a tetrameric complex composed of CRM1, importin alpha/beta dimer and the Venezuelan equine encephalitis virus (VEEV) capsid; this complex blocks the receptor-mediated transport through the nuclear pore.

Family&Domains:

Belongs to the exportin family.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

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CRM1 Antibody - #DF13364