CRM1 Antibody | Affinity Biosciences

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CRM1 Antibody - Western blot analysis of extracts from Hela cells, using CRM1 Antibody.

CRM1 Antibody - DF13364 staining Hela cells by IF/ICC.

CRM1 Antibody - Fig.

Product:	CRM1 Antibody
Catalog:	DF13364
Description:	Rabbit polyclonal antibody to CRM1
Application:	WB IF/ICC
Cited expt.:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	123kDa; 123kD(Calculated).
Uniprot:	O14980
RRID:	AB_2846383

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

CRM1 Antibody detects endogenous levels of total CRM1.

RRID:

AB_2846383
Cite Format: Affinity Biosciences Cat# DF13364, RRID:AB_2846383.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Chromosome region maintenance 1 protein homolog; CRM 1; CRM1 homolog; DKFZp686B1823; emb; Exp 1; Exp1; Exportin 1; Exportin-1; Exportin1; XPO 1; xpo1; XPO1_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human CRM1, corresponding to a region within the internal amino acids.

Uniprot:

O14980(XPO1_HUMAN) >>Visit HPA database.

Gene(ID):

XPO1(7514)

Expression:

O14980 XPO1_HUMAN:

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney.

Sequence:

O14980 XPO1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPAIMTMLADHAARQLLDFSQKLDINLLDNVVNCLYHGEGAQQRMAQEVLTHLKEHPDAWTRVDTILEFSQNMNTKYYGLQILENVIKTRWKILPRNQCEGIKKYVVGLIIKTSSDPTCVEKEKVYIGKLNMILVQILKQEWPKHWPTFISDIVGASRTSESLCQNNMVILKLLSEEVFDFSSGQITQVKSKHLKDSMCNEFSQIFQLCQFVMENSQNAPLVHATLETLLRFLNWIPLGYIFETKLISTLIYKFLNVPMFRNVSLKCLTEIAGVSVSQYEEQFVTLFTLTMMQLKQMLPLNTNIRLAYSNGKDDEQNFIQNLSLFLCTFLKEHDQLIEKRLNLRETLMEALHYMLLVSEVEETEIFKICLEYWNHLAAELYRESPFSTSASPLLSGSQHFDVPPRRQLYLPMLFKVRLLMVSRMAKPEEVLVVENDQGEVVREFMKDTDSINLYKNMRETLVYLTHLDYVDTERIMTEKLHNQVNGTEWSWKNLNTLCWAIGSISGAMHEEDEKRFLVTVIKDLLGLCEQKRGKDNKAIIASNIMYIVGQYPRFLRAHWKFLKTVVNKLFEFMHETHDGVQDMACDTFIKIAQKCRRHFVQVQVGEVMPFIDEILNNINTIICDLQPQQVHTFYEAVGYMIGAQTDQTVQEHLIEKYMLLPNQVWDSIIQQATKNVDILKDPETVKQLGSILKTNVRACKAVGHPFVIQLGRIYLDMLNVYKCLSENISAAIQANGEMVTKQPLIRSMRTVKRETLKLISGWVSRSNDPQMVAENFVPPLLDAVLIDYQRNVPAAREPEVLSTMAIIVNKLGGHITAEIPQIFDAVFECTLNMINKDFEEYPEHRTNFFLLLQAVNSHCFPAFLAIPPTQFKLVLDSIIWAFKHTMRNVADTGLQILFTLLQNVAQEEAAAQSFYQTYFCDILQHIFSVVTDTSHTAGLTMHASILAYMFNLVEEGKISTSLNPGNPVNNQIFLQEYVANLLKSAFPHLQDAQVKLFVTGLFSLNQDIPAFKEHLRDFLVQIKEFAGEDTSDLFLEEREIALRQADEEKHKRQMSVPGIFNPHEIPEEMCD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

O14980_XPO1_HUMAN

Function:

Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap.

(Microbial infection) Mediates the export of unspliced or incompletely spliced RNAs out of the nucleus from different viruses including HIV-1, HTLV-1 and influenza A. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization.

Subcellular Location:

Cytoplasm. Nucleus>Nucleoplasm. Nucleus>Cajal body. Nucleus>Nucleolus.
Note: Located in the nucleoplasm, Cajal bodies and nucleoli. Shuttles between the nucleus/nucleolus and the cytoplasm.

Tissue Specificity:

Family&Domains:

Belongs to the exportin family.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

References

1). XPO1 intensifies sorafenib resistance by stabilizing acetylation of NPM1 and enhancing epithelial-mesenchymal transition in hepatocellular carcinoma. BIOMEDICINE & PHARMACOTHERAPY, 2023 (PubMed: 36791564) [IF=6.9]

Application: IHC Species: human Sample:

Fig. 1. The expression and prognostic analysis of XPO1 gene in HCC. (A) and (B) Bioinformatics analysis was used to explore the expression of the XPO1 gene by using the GEO data (GSE36376, A) and TCGA-HCC (B) dataset. Data in (A) and (B) were analyzed by the Wilcox test. (C) The curve of risk score. More patient mortality corresponded to a higher risk score. (D) Kaplan-Meier survival analysis. (E) Immunohistochemical detection of XPO1 protein expression in para-carcinoma and carcinoma of HCC samples. bar= 100 µm. (F) XPO1 protein expression level was detected by Immunohistochemistry. Comparing the expression of XPO1 protein in carcinoma and para-carcinoma tissues in HCC. Data statistics using the χ2 test. P: Para-carcinoma; T: Carcinoma. * P

Application: WB Species: human Sample: HCC cell

Fig. 2. XPO1 promotes HCC progression and sorafenib resistance. (A) The expression of XPO1 in normal hepatocytes (HepaRG) and HCC cell lines (Hep3B, HepG2 and Huh7) by western Blotting. n = 3. (B) We used Western blot to verify the overexpression of XPO1. n = 3. (C) A soft agar colony formation assay was performed to evaluate the clonogenicity of HepG2/XPO1 KD cells. n = 3. (D) Transwell assay of XPO1 KD cells. bar= 20 µm. n = 3. (E) HepG2/XPO1 KD and HepG2/Vector cells were transplanted on nude mice, and tumor weight was measured. n = 3. (F) R analysis of the correlation between XPO1 and tumor proliferation signature in TCGA HCC dataset. (G) R analysis of XPO1 mRNA expression level using GEO dataset. (H) The IC50 values of Huh7 and Huh7/SR cells treated with sorafenib. n = 6. (I) Cells were transfected with mRFP-GFP-LC3 plasmid. Observation of autophagy by fluorescence microscope. bar= 5 µm. n = 3. (J) Detection of apoptosis level by Tunel assay. n = 3. (K) The IC50 values of Huh7/SR XPO1 KD and Huh7/SR Vector cells treated with sorafenib. n = 6.

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CRM1 Antibody - #DF13364