Product: FOXA2 Antibody
Catalog: DF13363
Description: Rabbit polyclonal antibody to FOXA2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 48kDa; 48kD(Calculated).
Uniprot: Q9Y261
RRID: AB_2846382

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
FOXA2 Antibody detects endogenous levels of total FOXA2.
RRID:
AB_2846382
Cite Format: Affinity Biosciences Cat# DF13363, RRID:AB_2846382.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Forkhead box A2; Forkhead box protein A2; FOX A2; foxa2; FOXA2_HUMAN; Hepatic nuclear factor 3 beta; Hepatocyte nuclear factor 3; Hepatocyte nuclear factor 3 beta; Hepatocyte nuclear factor 3-beta; HNF 3B; HNF-3-beta; HNF-3B; HNF3B; MGC19807; TCF 3B; TCF-3B; TCF3B; Transcription factor 3B;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MLGAVKMEGHEPSDWSSYYAEPEGYSSVSNMNAGLGMNGMNTYMSMSAAAMGSGSGNMSAGSMNMSSYVGAGMSPSLAGMSPGAGAMAGMGGSAGAAGVAGMGPHLSPSLSPLGGQAAGAMGGLAPYANMNSMSPMYGQAGLSRARDPKTYRRSYTHAKPPYSYISLITMAIQQSPNKMLTLSEIYQWIMDLFPFYRQNQQRWQNSIRHSLSFNDCFLKVPRSPDKPGKGSFWTLHPDSGNMFENGCYLRRQKRFKCEKQLALKEAAGAAGSGKKAAAGAQASQAQLGEAAGPASETPAGTESPHSSASPCQEHKRGGLGELKGTPAAALSPPEPAPSPGQQQQAAAHLLGPPHHPGLPPEAHLKPEHHYAFNHPFSINNLMSSEQQHHHSHHHHQPHKMDLKAYEQVMHYPGYGSPMPGSLAMGPVTNKTGLDASPLAADTSYYQGVYSRPIMNSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
88
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y261 As Substrate

Site PTM Type Enzyme
K6 Acetylation
S107 Phosphorylation O15111 (CHUK)
S111 Phosphorylation O15111 (CHUK)
T156 Phosphorylation P31749 (AKT1)
S212 Phosphorylation
S223 Phosphorylation
Y248 Phosphorylation
K256 Acetylation
K259 Acetylation
K264 Acetylation
K274 Acetylation
K275 Acetylation
S283 Phosphorylation
S295 Phosphorylation
T301 Phosphorylation
S303 Phosphorylation
S307 Phosphorylation
S309 Phosphorylation

Research Backgrounds

Function:

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.

PTMs:

Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner; in response to insulin signaling via AKT1 is exported from the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds DNA as a monomer. Binds TLE1 (By similarity). Interacts with FOXA1 and FOXA3. Interacts with PRKDC.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

References

1). Foxa2 attenuates steatosis and inhibits the NF-κB/IKK signaling pathway in nonalcoholic fatty liver disease. PeerJ, 2023 (PubMed: 38084145) [IF=2.7]

Application: WB    Species: Mouse    Sample:

Figure 1 Forkhead box a2 (Foxa2) expression is decreased in nonalcoholic fatty liver disease (NAFLD) mice. (A) Body- and liver- weight and the ratio of body/liver weight. (B) Fasting blood glucose (FBG). (C) Protein expression of Foxa2 in liver tissues of each group of mice. C57BL/6 mice were fed with normal diet and high-fat diet (HFD) for eight weeks. Then, HFD mice were injected with overexpression negative control (oe-NC) and Foxa2 (oe-Foxa2) in the tail vein once every two weeks for four weeks. *P < 0.05 and **P < 0.01 compared with Control; #P < 0.05 and ##P < 0.01 compared with HFD.

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