Phospho-HDAC5 (Ser259) Antibody - #AF3490
| Product: | Phospho-HDAC5 (Ser259) Antibody |
| Catalog: | AF3490 |
| Description: | Rabbit polyclonal antibody to Phospho-HDAC5 (Ser259) |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 124kDa; 122kD(Calculated). |
| Uniprot: | Q9UQL6 |
| RRID: | AB_2834928 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF3490, RRID:AB_2834928.
Fold/Unfold
Antigen NY CO 9; Antigen NY-CO-9; HD5; HDAC 5; HDAC5; HDAC5_HUMAN; Histone deacetylase 5; NY CO 9;
Immunogens
- Q9UQL6 HDAC5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNSPNESDGMSGREPSLEILPRTSLHSIPVTVEVKPVLPRAMPSSMGGGGGGSPSPVELRGALVGSVDPTLREQQLQQELLALKQQQQLQKQLLFAEFQKQHDHLTRQHEVQLQKHLKQQQEMLAAKQQQEMLAAKRQQELEQQRQREQQRQEELEKQRLEQQLLILRNKEKSKESAIASTEVKLRLQEFLLSKSKEPTPGGLNHSLPQHPKCWGAHHASLDQSSPPQSGPPGTPPSYKLPLPGPYDSRDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGTVISTFKKRAVEITGAGPGASSVCNSAPGSGPSSPNSSHSTIAENGFTGSVPNIPTEMLPQHRALPLDSSPNQFSLYTSPSLPNISLGLQATVTVTNSHLTASPKLSTQQEAERQALQSLRQGGTLTGKFMSTSSIPGCLLGVALEGDGSPHGHASLLQHVLLLEQARQQSTLIAVPLHGQSPLVTGERVATSMRTVGKLPRHRPLSRTQSSPLPQSPQALQQLVMQQQHQQFLEKQKQQQLQLGKILTKTGELPRQPTTHPEETEEELTEQQEVLLGEGALTMPREGSTESESTQEDLEEEDEEDDGEEEEDCIQVKDEEGESGAEEGPDLEEPGAGYKKLFSDAQPLQPLQVYQAPLSLATVPHQALGRTQSSPAAPGGMKSPPDQPVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEHAGRIQSIWSRLQETGLLSKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGPISQKMYAVLPCGGIGVDSDTVWNEMHSSSAVRMAVGCLLELAFKVAAGELKNGFAIIRPPGHHAEESTAMGFCFFNSVAITAKLLQQKLNVGKVLIVDWDIHHGNGTQQAFYNDPSVLYISLHRYDNGNFFPGSGAPEEVGGGPGVGYNVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAHEFSPDVVLVSAGFDAVEGHLSPLGGYSVTARCFGHLTRQLMTLAGGRVVLALEGGHDLTAICDASEACVSALLSVELQPLDEAVLQQKPNINAVATLEKVIEIQSKHWSCVQKFAAGLGRSLREAQAGETEEAETVSAMALLSVGAEQAQAAAAREHSPRPAEEPMEQEPAL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UQL6 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S3 | Phosphorylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| K35 | Sumoylation | Uniprot | |
| S53 | Phosphorylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| T70 | Phosphorylation | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| S220 | Phosphorylation | Uniprot | |
| T234 | Phosphorylation | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| S259 | Phosphorylation | O43318 (MAP3K7) , Q05655 (PRKCD) , Q13555 (CAMK2G) , Q7KZI7 (MARK2) , Q9UQM7 (CAMK2A) , Q16566 (CAMK4) , P57059 (SIK1) , Q15139 (PRKD1) , Q13131 (PRKAA1) , Q14012 (CAMK1) , P54646 (PRKAA2) | Uniprot |
| S278 | Phosphorylation | Q96GD4 (AURKB) | Uniprot |
| S279 | Phosphorylation | Q9Y463 (DYRK1B) , P17612 (PRKACA) , Q00535 (CDK5) | Uniprot |
| T288 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| T292 | Phosphorylation | Q16512 (PKN1) | Uniprot |
| S368 | Phosphorylation | Uniprot | |
| S469 | Phosphorylation | Uniprot | |
| T496 | Phosphorylation | Uniprot | |
| S498 | Phosphorylation | P54646 (PRKAA2) , Q14012 (CAMK1) , Q16566 (CAMK4) , Q13555 (CAMK2G) , Q13557 (CAMK2D) , Q9UQM7 (CAMK2A) , P57059 (SIK1) , Q15139 (PRKD1) , O94806 (PRKD3) , Q13131 (PRKAA1) , Q9BZL6 (PRKD2) | Uniprot |
| S504 | Phosphorylation | Uniprot | |
| K533 | Acetylation | Uniprot | |
| K533 | Sumoylation | Uniprot | |
| K537 | Ubiquitination | Uniprot | |
| T546 | Phosphorylation | Uniprot | |
| T547 | Phosphorylation | Uniprot | |
| K605 | Sumoylation | Uniprot | |
| S611 | Phosphorylation | Uniprot | |
| S661 | Phosphorylation | Uniprot | |
| S671 | Phosphorylation | Uniprot | |
| S755 | Phosphorylation | Uniprot | |
| Y776 | Phosphorylation | Uniprot | |
| S798 | Phosphorylation | Uniprot | |
| S799 | Phosphorylation | Uniprot | |
| S1071 | Phosphorylation | Uniprot | |
| S1108 | Phosphorylation | Uniprot |
Research Backgrounds
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Serves as a corepressor of RARA and causes its deacetylation. In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response.
Phosphorylated by AMPK, CaMK1, SIK1 and PRKD1 at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm and inhibition. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Phosphorylated by GRK5, leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription (By similarity).
Ubiquitinated. Polyubiquitination however does not lead to its degradation.
Nucleus. Cytoplasm.
Note: Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by AMPK, CaMK1 and SIK1.
Ubiquitous.
Interacts with AHRR, BAHD1, BCOR, HDAC7, HDAC9, CTBP1, MEF2C, NCOR2, NRIP1, PHB2 and a 14-3-3 chaperone protein. Interacts with BCL6, DDIT3/CHOP, GRK5, KDM5B and MYOCD. Interacts with EP300 in the presence of TFAP2C. Interacts with ANKRA2. Interacts with CUL7 (as part of the 3M complex); negatively regulated by ANKRA2. Interacts with ZBTB7B; the interaction allows the recruitment of HDAC4 on CD8 loci for deacetylation and possible inhibition of CD8 genes expression (By similarity). Interacts with RARA.
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
Belongs to the histone deacetylase family. HD type 2 subfamily.
Research Fields
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Human Diseases > Substance dependence > Alcoholism.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.