DFFB Antibody - #DF13360

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Product: DFFB Antibody
Catalog: DF13360
Description: Rabbit polyclonal antibody to DFFB
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog
Mol.Wt.: 39kDa; 39kD(Calculated).
Uniprot: O76075
RRID: AB_2846379

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Zebrafish(83%), Bovine(88%), Horse(88%), Sheep(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
DFFB Antibody detects endogenous levels of total DFFB.
RRID:
AB_2846379
Cite Format: Affinity Biosciences Cat# DF13360, RRID:AB_2846379.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CAD; Caspase activated deoxyribonuclease; Caspase activated DNase; Caspase activated nuclease; Caspase-activated deoxyribonuclease; Caspase-activated DNase; Caspase-activated nuclease; CPAN; Deoxyribonuclease III, caspase activated; DFF 40; DFF-40; DFF2; DFF40; Dffb; DFFB_HUMAN; Didff; DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor subunit beta; DNA fragmentation factor, 40 Da, beta polypeptide (caspase activated DNase); DNA fragmentation factor, 40 kD beta subunit; DNA fragmentation factor, 40 kD, beta polypeptide; DNA fragmentation factor, 40kDa, beta polypeptide (caspase activated DNase); OTTHUMP00000003633;

Immunogens

Immunogen:
Uniprot:

O76075(DFFB_HUMAN) >>Visit HPA database.

Gene(ID):
DFFB(1677)
Sequence:
MLQKPKSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTELTEDYFPSVPDNAELVLLTLGQAWQGYVSDIRRFLSAFHEPQVGLIQAAQQLLCDEQAPQRQRLLADLLHNVSQNIAAETRAEDPPWFEGLESRFQSKSGYLRYSCESRIRSYLREVSSYPSTVGAEAQEEFLRVLGSMCQRLRSMQYNGSYFDRGAKGGSRLCTPEGWFSCQGPFDMDSCLSRHSINPYSNRESRILFSTWNLDHIIEKKRTIIPTLVEAIKEQDGREVDWEYFYGLLFTSENLKLVHIVCHKKTTHKLNCDPSRIYKPQTRLKRKQPVRKRQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Horse
88
Bovine
88
Sheep
88
Dog
88
Zebrafish
83
Xenopus
75
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O76075 As Substrate

Site PTM Type Enzyme
K152 Ubiquitination
Y158 Phosphorylation
K264 Ubiquitination
K265 Ubiquitination
K277 Ubiquitination
S319 Phosphorylation
Y322 Phosphorylation
K323 Ubiquitination
T326 Phosphorylation

Research Backgrounds

Function:

Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer of DFFA and DFFB. Interacts with H1-1.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

References

1). Downregulation of the Long Noncoding RNA IALNCR Targeting MAPK8/JNK1 Promotes Apoptosis and Antagonizes Bovine Viral Diarrhea Virus Replication in Host Cells. Journal of Virology, 2022 (PubMed: 35993735) [IF=5.4]

Application: WB    Species: Bovine    Sample: MDBK cells and BT cell

FIG 3 mRNA levels of apoptosis-related genes caspase-3 and Bcl-2 (A), and Bax and Bak1 (B) detected by qRT-PCR at different time points after BVDV infection (*, P < 0.05; **, P < 0.01; ***, P < 0.001). (C) Protein levels of caspase-3 and Bcl-2 were detected by Western blotting at different time points after BVDV infection. Lowercase letters (a versus b; b versus c) indicate a significant difference of P < 0.05; a versus c indicates a significant difference of P < 0.01. (D) Protein levels of apoptosis-related pathway proteins were detected by Western blotting (***, P < 0.001). (E) Identification of procaspase-3/8/9 cleavage, ICAD/DFF45 degradation, nuclear translocation of active caspase-3 and CAD/DFF40, and nuclear PARP cleavage by Western blotting in BVDV-infected MDBK cells and BT cells at different time points after infection.

Restrictive clause

 

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