DNase II Antibody - #DF13359
| Product: | DNase II Antibody |
| Catalog: | DF13359 |
| Description: | Rabbit polyclonal antibody to DNase II |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| Mol.Wt.: | 39kDa; 40kD(Calculated). |
| Uniprot: | O00115 |
| RRID: | AB_2846378 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13359, RRID:AB_2846378.
Fold/Unfold
Acid DNase; Deoxyribonuclease 2 alpha; Deoxyribonuclease 2; Deoxyribonuclease II alpha; Deoxyribonuclease II; Deoxyribonuclease II lysosomal; Deoxyribonuclease-2-alpha; Deoxyribonuclease2; DeoxyribonucleaseII; DNASE 2; DNASE 2A; DNase II alpha; DNase II lysosomal; DNASE2; DNASE2A; DNaseII; DNL 2; DNL; DNL2; DNS2A_HUMAN; Lysosomal DNase II; R31240 2; R31240_2;
Immunogens
- O00115 DNS2A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MIPLLLAALLCVPAGALTCYGDSGQPVDWFVVYKLPALRGSGEAAQRGLQYKYLDESSGGWRDGRALINSPEGAVGRSLQPLYRSNTSQLAFLLYNDQPPQPSKAQDSSMRGHTKGVLLLDHDGGFWLVHSVPNFPPPASSAAYSWPHSACTYGQTLLCVSFPFAQFSKMGKQLTYTYPWVYNYQLEGIFAQEFPDLENVVKGHHVSQEPWNSSITLTSQAGAVFQSFAKFSKFGDDLYSGWLAAALGTNLQVQFWHKTVGILPSNCSDIWQVLNVNQIAFPGPAGPSFNSTEDHSKWCVSPKGPWTCVGDMNRNQGEEQRGGGTLCAQLPALWKAFQPLVKNYQPCNGMARKPSRAYKI
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O00115 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y20 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| N212 | N-Glycosylation | Uniprot | |
| R321 | Methylation | Uniprot | |
| K335 | Acetylation | Uniprot | |
| K353 | Acetylation | Uniprot | |
| S355 | Phosphorylation | Uniprot |
Research Backgrounds
Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.
Glycosylated. Mutations that eliminate N-glycosylation sites reduce activity, but enzymatic deglycosylation has no effect.
Lysosome.
Belongs to the DNase II family.
Research Fields
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
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