Endomucin Antibody - #DF13357

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Product: Endomucin Antibody
Catalog: DF13357
Description: Rabbit polyclonal antibody to Endomucin
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 27kDa; 27kD(Calculated).
Uniprot: Q9ULC0
RRID: AB_2846376

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Endomucin Antibody detects endogenous levels of total Endomucin.
RRID:
AB_2846376
Cite Format: Affinity Biosciences Cat# DF13357, RRID:AB_2846376.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EMCN; EMCN2; Endomucin 2; Endomucin; Endomucin-2; Endothelial sialomucin; Gastric cancer antigen Ga34; MUC-14; MUC14; MUCEN_HUMAN; Mucin 14; Mucin-14;

Immunogens

Immunogen:
Uniprot:

Q9ULC0(MUCEN_HUMAN) >>Visit HPA database.

Gene(ID):
EMCN(51705)
Expression:
Q9ULC0 MUCEN_HUMAN:

Expressed in heart, kidney and lung.

Sequence:
MELLQVTILFLLPSICSSNSTGVLEAANNSLVVTTTKPSITTPNTESLQKNVVTPTTGTTPKGTITNELLKMSLMSTATFLTSKDEGLKATTTDVRKNDSIISNVTVTSVTLPNAVSTLQSSKPKTETQSSIKTTEIPGSVLQPDASPSKTGTLTSIPVTIPENTSQSQVIGTEGGKNASTSATSRSYSSIILPVVIALIVITLSVFVLVGLYRMCWKADPGTPENGNDQPQSDKESVKLLTVKTISHESGEHSAQGKTKN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9ULC0 As Substrate

Site PTM Type Enzyme
S76 Phosphorylation
K84 Acetylation
K89 Acetylation
T173 Phosphorylation
S180 Phosphorylation
T181 Phosphorylation
S182 Phosphorylation

Research Backgrounds

Function:

Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix.

PTMs:

Highly O-glycosylated. Sialic acid-rich glycoprotein.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Membrane>Single-pass type I membrane protein.
Note: Consistently localized to the plasma membrane and less abundantly to Golgi-like perinuclear stacks.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in heart, kidney and lung.

References

1). A vessel subtype beneficial for osteogenesis enhanced by strontium-doped sodium titanate nanorods by modulating macrophage polarization. Journal of Materials Chemistry B, 2020 (PubMed: 32627795) [IF=7.0]
2). Chronic alcohol reduces bone mass through inhibiting proliferation and promoting aging of endothelial cells in type-H vessels. STEM CELLS AND DEVELOPMENT, 2022 (PubMed: 35491665) [IF=4.0]

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