SLC31A1 / CTR1 Antibody - #DF13356

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Product: SLC31A1 / CTR1 Antibody
Catalog: DF13356
Description: Rabbit polyclonal antibody to SLC31A1 / CTR1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Horse, Rabbit, Dog
Mol.Wt.: 21kDa; 21kD(Calculated).
Uniprot: O15431
RRID: AB_2846375

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(100%), Rabbit(100%), Dog(88%)
Clonality:
Polyclonal
Specificity:
SLC31A1 / CTR1 Antibody detects endogenous levels of total SLC31A1 / CTR1.
RRID:
AB_2846375
Cite Format: Affinity Biosciences Cat# DF13356, RRID:AB_2846375.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Copper transport 1 homolog; Copper transporter 1; COPT1; COPT1_HUMAN; CTR1; hCTR1; High affinity copper uptake protein 1; SLC31A1; solute carrier family 31 (copper tansporters) member 1; Solute carrier family 31 member 1;

Immunogens

Immunogen:
Uniprot:

O15431(COPT1_HUMAN) >>Visit HPA database.

Gene(ID):
SLC31A1(1317)
Sequence:
MDHSHHMGMSYMDSNSTMQPSHHHPTTSASHSHGGGDSSMMMMPMTFYFGFKNVELLFSGLVINTAGEMAGAFVAVFLLAMFYEGLKIARESLLRKSQVSIRYNSMPVPGPNGTILMETHKTVGQQMLSFPHLLQTVLHIIQVVISYFLMLIFMTYNGYLCIAVAAGAGTGYFLFSWKKAVVVDITEHCH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
100
Dog
88
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15431 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
S10 Phosphorylation
Y11 Phosphorylation
N15 N-Glycosylation
T27 O-Glycosylation
K96 Ubiquitination
S105 Phosphorylation
T114 Phosphorylation

Research Backgrounds

Function:

High-affinity, saturable copper transporter involved in dietary copper uptake.

PTMs:

O-Glycosylation at Thr-27 protects from proteolytic cleavage in the N-terminal extracellular domain.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: Localizes to the apical membrane in intestinal epithelial cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotrimer.

Family&Domains:

Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Organismal Systems > Digestive system > Mineral absorption.

References

1). Comprehensive Gene Analysis Reveals Cuproptosis-Related Gene Signature Associated with M2 Macrophage in Staphylococcus aureus-Infected Osteomyelitis. Journal of inflammation research, 2024 (PubMed: 38770176) [IF=4.5]
2). Bioinformatics analysis and experimental validation of m6A and cuproptosis-related lncRNA NFE4 in clear cell renal cell carcinoma. Discover oncology, 2024 (PubMed: 38797784) [IF=2.2]

Application: WB    Species: Human    Sample: Caki-1 and OS-RC-2 cells

Figure. 9 Functional experiments against NFE4 in Caki-1 and OS-RC-2 cells. A NFE4 was highly expressed in tumor tissues. B The expression of NFE4 in renal cancer cells was measured using real-time quantitative reverse transcription-PCR. C Survival analysis of the high NFE4 group and low NFE4 group. D, E The mRNA expression of NFE4 in Caki-1 and OS-RC-2 cells was measured using real-time quantitative reverse transcription-PCR. F, G Cell Counting Kit-8 assays were used to detect the effect of NFE4 knockdown on cell proliferation in Caki-1 and OS-RC-2 cells. H qRT-PCR shows the expression and regulation of NFE4 in Caki-1 and OS-RC-2 cells treated with drugs that induce cuproptosis for 24 h (n = 3). CuCl2 (2mM), Elesclomol (20 nM), both CuCl2 (2mM) and Elesclomol (20 nM). I Western blotting was used to determine the protein levels of ATP7B and SLC31A1 in Caki-1 and OS-RC-2 cells. One-way ANOVA, NC was used as a control group. Data are shown as the mean ± standard deviation from three independent experiments and were compared to the respective si-NC group. ****P < 0.0001; ***P < 0.001; **P < 0.01 and *P < 0.05.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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