PGAM5 Antibody | Affinity Biosciences

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Western blot analysis on various cells lysates using PGAM5 Antibody.

DF13355 at 1/100 staining Rat heart tissue by IHC-P.

Product:	PGAM5 Antibody
Catalog:	DF13355
Description:	Rabbit polyclonal antibody to PGAM5
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	32KD; 32kD(Calculated).
Uniprot:	Q96HS1
RRID:	AB_2846374

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

PGAM5 Antibody detects endogenous levels of total PGAM5.

RRID:

AB_2846374
Cite Format: Affinity Biosciences Cat# DF13355, RRID:AB_2846374.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Bcl-XL-binding protein v68; BXLBv68; MGC5352; mitochondrial; PGAM5; PGAM5_HUMAN; Phosphoglycerate mutase family member 5; Serine/threonine protein phosphatase PGAM5 mitochondrial; Serine/threonine-protein phosphatase PGAM5;

Immunogens

Immunogen:

Uniprot:

Q96HS1(PGAM5_HUMAN) >>Visit HPA database.

Gene(ID):

PGAM5(192111)

Sequence:

Q96HS1 PGAM5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAFRQALQLAACGLAGGSAAVLFSAVAVGKPRAGGDAEPRPAEPPAWAGGARPGPGVWDPNWDRREPLSLINVRKRNVESGEEELASKLDHYKAKATRHIFLIRHSQYHVDGSLEKDRTLTPLGREQAELTGLRLASLGLKFNKIVHSSMTRAIETTDIISRHLPGVCKVSTDLLREGAPIEPDPPVSHWKPEAVQYYEDGARIEAAFRNYIHRADARQEEDSYEIFICHANVIRYIVCRALQFPPEGWLRLSLNNGSITHLVIRPNGRVALRTLGDTGFMPPDKITRS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Zebrafish

Pig

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96HS1 As Substrate

Q96HS1

Site	PTM Type	Source
K75	Acetylation	Uniprot
K75	Ubiquitination	Uniprot
S80	Phosphorylation	Uniprot
S87	Phosphorylation	Uniprot
K88	Acetylation	Uniprot
K88	Ubiquitination	Uniprot
K93	Acetylation	Uniprot
K93	Ubiquitination	Uniprot
Y108	Phosphorylation	Uniprot
K116	Acetylation	Uniprot
T131	Phosphorylation	Uniprot
K141	Acetylation	Uniprot
K141	Ubiquitination	Uniprot
K144	Acetylation	Uniprot
K144	Ubiquitination	Uniprot
C168	S-Nitrosylation	Uniprot
K169	Ubiquitination	Uniprot
S171	Phosphorylation	Uniprot
K191	Acetylation	Uniprot
K191	Ubiquitination	Uniprot
S253	Phosphorylation	Uniprot
K285	Ubiquitination	Uniprot

Research Backgrounds

Q96HS1_PGAM5_HUMAN

Function:

Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression. Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore.

PTMs:

Both isoform 1 and isoform 2 are phosphorylated by the RIPK1/RIPK3 complex under necrotic conditions. This phosphorylation increases PGAM5 phosphatase activity.

Subcellular Location:

Mitochondrion outer membrane>Single-pass membrane protein.
Note: Isoform 2 overexpression results in the formation of disconnected punctuate mitochondria distributed throughout the cytoplasm. Isoform 1 overexpression results in the clustering of mitochondria around the nucleus.

Subunit Structure:

Dimer. Forms a ternary complex with NFE2L2 and KEAP1. Interacts with BCL2L1 and MAP3K5. Upon TNF-induced necrosis, forms in complex with RIPK1, RIPK3 and MLKL; the formation of this complex leads to PGAM5 phosphorylation. Isoform 2, but not isoform 1, interacts with DNM1L; this interaction leads to DNM1L dephosphorylation and activation and eventually to mitochondria fragmentation.

Family&Domains:

The N-terminal 35 amino acids, including the potential transmembrane alpha-helix, function as a non-cleaved mitochondrial targeting sequence that targets the protein to the cytosolic side of the outer mitochondrial membrane.

Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

References

1). Pterocephin A, a Novel Triterpenoid Saponin from Pterocephalus hookeri Induced Liver Injury by Activation of Necroptosis. PHYTOMEDICINE, 2021 (PubMed: 33831690) [IF=7.9]

Application: WB Species: Human Sample: L-02 cells

Fig 4. PA activated RIP1 signaling pathway. A. Western blot analysis for RIP1 signaling pathway in L-02 cells with PA treatment; B. Western blot analysis for RIP1 signaling pathway with the interference of Nec-1 in L-02 cells. For A: Data were represented as mean ± SD (n = 3), * p < 0.05, ** p < 0.01 compared with the Con group. For B: Data were represented as mean ± SD (n = 3); # p < 0.05, ## p < 0.01 compared with the Con group; * p < 0.05, ** p < 0.01 compared with the PA- 4 group.

2). Vascular endothelial cell damage induced by ox-LDL through AMPK-Nrf2/Keap1-PGAM5 signaling axis. Research Square, 2022

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PGAM5 Antibody - #DF13355