TET3 Antibody - #DF13335
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| Product: | TET3 Antibody |
| Catalog: | DF13335 |
| Description: | Rabbit polyclonal antibody to TET3 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 179kDa; 194kD(Calculated). |
| Uniprot: | O43151 |
| RRID: | AB_2846354 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13335, RRID:AB_2846354.
Fold/Unfold
hCG_40738; KIAA0401; Methylcytosine dioxygenase TET3; Probable methylcytosine dioxygenase TET3; Tet methylcytosine dioxygenase 3; Tet oncogene family member 3; Tet3; TET3_HUMAN;
Immunogens
- O43151 TET3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSQFQVPLAVQPDLPGLYDFPQRQVMVGSFPGSGLSMAGSESQLRGGGDGRKKRKRCGTCEPCRRLENCGACTSCTNRRTHQICKLRKCEVLKKKVGLLKEVEIKAGEGAGPWGQGAAVKTGSELSPVDGPVPGQMDSGPVYHGDSRQLSASGVPVNGAREPAGPSLLGTGGPWRVDQKPDWEAAPGPAHTARLEDAHDLVAFSAVAEAVSSYGALSTRLYETFNREMSREAGNNSRGPRPGPEGCSAGSEDLDTLQTALALARHGMKPPNCNCDGPECPDYLEWLEGKIKSVVMEGGEERPRLPGPLPPGEAGLPAPSTRPLLSSEVPQISPQEGLPLSQSALSIAKEKNISLQTAIAIEALTQLSSALPQPSHSTPQASCPLPEALSPPAPFRSPQSYLRAPSWPVVPPEEHSSFAPDSSAFPPATPRTEFPEAWGTDTPPATPRSSWPMPRPSPDPMAELEQLLGSASDYIQSVFKRPEALPTKPKVKVEAPSSSPAPAPSPVLQREAPTPSSEPDTHQKAQTALQQHLHHKRSLFLEQVHDTSFPAPSEPSAPGWWPPPSSPVPRLPDRPPKEKKKKLPTPAGGPVGTEKAAPGIKPSVRKPIQIKKSRPREAQPLFPPVRQIVLEGLRSPASQEVQAHPPAPLPASQGSAVPLPPEPSLALFAPSPSRDSLLPPTQEMRSPSPMTALQPGSTGPLPPADDKLEELIRQFEAEFGDSFGLPGPPSVPIQDPENQQTCLPAPESPFATRSPKQIKIESSGAVTVLSTTCFHSEEGGQEATPTKAENPLTPTLSGFLESPLKYLDTPTKSLLDTPAKRAQAEFPTCDCVEQIVEKDEGPYYTHLGSGPTVASIRELMEERYGEKGKAIRIEKVIYTGKEGKSSRGCPIAKWVIRRHTLEEKLLCLVRHRAGHHCQNAVIVILILAWEGIPRSLGDTLYQELTDTLRKYGNPTSRRCGLNDDRTCACQGKDPNTCGASFSFGCSWSMYFNGCKYARSKTPRKFRLAGDNPKEEEVLRKSFQDLATEVAPLYKRLAPQAYQNQVTNEEIAIDCRLGLKEGRPFAGVTACMDFCAHAHKDQHNLYNGCTVVCTLTKEDNRCVGKIPEDEQLHVLPLYKMANTDEFGSEENQNAKVGSGAIQVLTAFPREVRRLPEPAKSCRQRQLEARKAAAEKKKIQKEKLSTPEKIKQEALELAGITSDPGLSLKGGLSQQGLKPSLKVEPQNHFSSFKYSGNAVVESYSVLGNCRPSDPYSMNSVYSYHSYYAQPSLTSVNGFHSKYALPSFSYYGFPSSNPVFPSQFLGPGAWGHSGSSGSFEKKPDLHALHNSLSPAYGGAEFAELPSQAVPTDAHHPTPHHQQPAYPGPKEYLLPKAPLLHSVSRDPSPFAQSSNCYNRSIKQEPVDPLTQAEPVPRDAGKMGKTPLSEVSQNGGPSHLWGQYSGGPSMSPKRTNGVGGSWGVFSSGESPAIVPDKLSSFGASCLAPSHFTDGQWGLFPGEGQQAASHSGGRLRGKPWSPCKFGNSTSALAGPSLTEKPWALGAGDFNSALKGSPGFQDKLWNPMKGEEGRIPAAGASQLDRAWQSFGLPLGSSEKLFGALKSEEKLWDPFSLEEGPAEEPPSKGAVKEEKGGGGAEEEEEELWSDSEHNFLDENIGGVAVAPAHGSILIECARRELHATTPLKKPNRCHPTRISLVFYQHKNLNQPNHGLALWEAKMKQLAERARARQEEAARLGLGQQEAKLYGKKRKWGGTVVAEPQQKEKKGVVPTRQALAVPTDSAVTVSSYAYTKVTGPYSRWI
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O43151 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S3 | Phosphorylation | Uniprot | |
| Y7 | Phosphorylation | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| Y78 | Phosphorylation | Uniprot | |
| T83 | Phosphorylation | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| T306 | Phosphorylation | Uniprot | |
| T310 | Phosphorylation | Uniprot | |
| K356 | Sumoylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| T545 | Phosphorylation | Uniprot | |
| T555 | Phosphorylation | Uniprot | |
| S612 | Phosphorylation | Uniprot | |
| T657 | Phosphorylation | Uniprot | |
| S666 | Phosphorylation | Uniprot | |
| T673 | Phosphorylation | Uniprot | |
| K676 | Ubiquitination | Uniprot | |
| S677 | Phosphorylation | Uniprot | |
| T681 | Phosphorylation | Uniprot | |
| K684 | Ubiquitination | Uniprot | |
| K859 | Ubiquitination | Uniprot | |
| K884 | Ubiquitination | Uniprot | |
| K898 | Ubiquitination | Uniprot | |
| K1040 | Acetylation | Uniprot | |
| T1048 | Phosphorylation | Uniprot | |
| K1053 | Sumoylation | Uniprot | |
| K1053 | Ubiquitination | Uniprot | |
| K1080 | Ubiquitination | Uniprot | |
| S1248 | Phosphorylation | Uniprot | |
| K1262 | Sumoylation | Uniprot | |
| K1262 | Ubiquitination | Uniprot | |
| T1285 | Phosphorylation | Uniprot | |
| S1297 | Phosphorylation | Uniprot | |
| Y1303 | Phosphorylation | Uniprot | |
| S1379 | Phosphorylation | Uniprot | |
| S1409 | Phosphorylation | Uniprot | |
| K1462 | Ubiquitination | Uniprot | |
| K1466 | Ubiquitination | Uniprot | |
| K1544 | Ubiquitination | Uniprot | |
| K1577 | Ubiquitination | Uniprot | |
| K1603 | Sumoylation | Uniprot | |
| K1603 | Ubiquitination | Uniprot | |
| K1610 | Ubiquitination | Uniprot | |
| T1630 | Phosphorylation | Uniprot | |
| T1638 | Phosphorylation | Uniprot | |
| S1640 | Phosphorylation | Uniprot |
Research Backgrounds
Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation (By similarity). Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin (By similarity). In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Binds preferentially to DNA containing cytidine-phosphate-guanosine (CpG) dinucleotides over CpH (H=A, T, and C), hemimethylated-CpG and hemimethylated-hydroxymethyl-CpG.
Nucleus. Cytoplasm. Chromosome.
Note: At the zygotic stage, localizes in the male pronucleus, while it localizes to the cytoplasm at other preimplantation stages. Binds to the promoter of target genes, close to the transcription start site.
Expressed in colon, muscle, adrenal gland and peripheral blood lymphocytes.
Interacts with HCFC1 and OGT.
The CXXC zinc finger mediates binding to CpG-DNA (PubMed:29276034). It mediates binding to DNA sequences containing unmethylated cytosine or 5-carboxylcytosine in 5'-CCG-3' DNA sequence motifs (By similarity).
Belongs to the TET family.
References
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