Glutaminase Antibody - #DF13334

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Product: Glutaminase Antibody
Catalog: DF13334
Description: Rabbit polyclonal antibody to Glutaminase
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 73kDa; 73kD(Calculated).
Uniprot: O94925
RRID: AB_2846353

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Glutaminase Antibody detects endogenous levels of total Glutaminase.
RRID:
AB_2846353
Cite Format: Affinity Biosciences Cat# DF13334, RRID:AB_2846353.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AAD20; DKFZp686O15119; FLJ10358; GAC; GAM; GLS; GLS1; GLSK_HUMAN; Glutaminase C; Glutaminase kidney isoform; Glutaminase phosphate activated; K-glutaminase; KGA; KIAA0838; L-glutamine amidohydrolase; mitochondrial;

Immunogens

Immunogen:
Uniprot:

O94925(GLSK_HUMAN) >>Visit HPA database.

Gene(ID):
GLS(2744)
Expression:
O94925 GLSK_HUMAN:

Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle.

Sequence:
MMRLRGSGMLRDLLLRSPAGVSATLRRAQPLVTLCRRPRGGGRPAAGPAAAARLHPWWGGGGWPAEPLARGLSSSPSEILQELGKGSTHPQPGVSPPAAPAAPGPKDGPGETDAFGNSEGKELVASGENKIKQGLLPSLEDLLFYTIAEGQEKIPVHKFITALKSTGLRTSDPRLKECMDMLRLTLQTTSDGVMLDKDLFKKCVQSNIVLLTQAFRRKFVIPDFMSFTSHIDELYESAKKQSGGKVADYIPQLAKFSPDLWGVSVCTVDGQRHSTGDTKVPFCLQSCVKPLKYAIAVNDLGTEYVHRYVGKEPSGLRFNKLFLNEDDKPHNPMVNAGAIVVTSLIKQGVNNAEKFDYVMQFLNKMAGNEYVGFSNATFQSERESGDRNFAIGYYLKEKKCFPEGTDMVGILDFYFQLCSIEVTCESASVMAATLANGGFCPITGERVLSPEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSGVAGGILLVVPNVMGMMCWSPPLDKMGNSVKGIHFCHDLVSLCNFHNYDNLRHFAKKLDPRREGGDQRVKSVINLLFAAYTGDVSALRRFALSAMDMEQRDYDSRTALHVAAAEGHVEVVKFLLEACKVNPFPKDRWNNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDNGKENQTVHKNLDGLL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Bovine
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O94925 As Substrate

Site PTM Type Enzyme
S17 Phosphorylation
S95 Phosphorylation
T112 Phosphorylation
S118 Phosphorylation
K121 Ubiquitination
K158 Acetylation
K158 Ubiquitination
K164 Acetylation
K164 Ubiquitination
K176 Acetylation
Y249 Phosphorylation
K289 Ubiquitination
T302 Phosphorylation
Y304 Phosphorylation
K311 Acetylation
K311 Ubiquitination
K320 Acetylation
Y357 Phosphorylation
Y370 Phosphorylation
R387 Methylation
Y393 Phosphorylation
Y394 Phosphorylation
S449 Phosphorylation
S523 Phosphorylation

Research Backgrounds

Function:

Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain.

Lacks catalytic activity.

PTMs:

Synthesized as a 74-kDa cytosolic precursor which is proteolytically processed by the mitochondrial-processing peptidase (MPP) via a 72-kDa intermediate to yield the mature mitochondrial 68- and 65-kDa subunits.

Subcellular Location:

Mitochondrion. Cytoplasm>Cytosol.
Note: The 74-kDa cytosolic precursor is translocated into the mitochondria and processed via a 72-kDa intermediate to yield the mature 68- and 65-kDa subunits.

Mitochondrion.

Mitochondrion matrix.
Note: Produced by the proteolytic processing of the 74-kDa cytosolic precursor.

Mitochondrion matrix.
Note: Produced by the proteolytic processing of the 74-kDa cytosolic precursor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle.

Subunit Structure:

Homotetramer, dimer of dimers. The tetramers can assemble into rod-like oligomers (in vitro), but the physiological significance of this is not clear (By similarity). Interacts with RAF1 and MAP2K2. Interacts with ATCAY; the interaction is direct and may control GLS localization, negatively regulating its activity.

Family&Domains:

The C-terminal ANK repeats prevent the assembly of the supra-tetrameric filaments.

A highly mobile activation loop at the dimer-dimer interface is important for enzyme activity.

Belongs to the glutaminase family.

Research Fields

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Metabolism > Amino acid metabolism > Arginine biosynthesis.

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

· Metabolism > Metabolism of other amino acids > D-Glutamine and D-glutamate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Nervous system > GABAergic synapse.

· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.

References

1). The cuproptosis-related gene glutaminase promotes alveolar macrophage copper ion accumulation in chronic obstructive pulmonary disease. International immunopharmacology, 2024 (PubMed: 38325045) [IF=5.6]
2). Tripartite motif containing 33 demonstrated anticancer effect by degrading c‑Myc: Limitation of glutamine metabolism and proliferation in endometrial carcinoma cells. International journal of oncology, 2023 (PubMed: 37859625) [IF=5.2]

Application: WB    Species: Human    Sample: EC cell

Figure 4 TRIM33 overexpression inhibits the glutamine metabolism in HEC-1-A and AN3CA cells in vitro. (A) Glutamine uptake and (B) intracellular glutamate production of TRIM33-silenced or -overexpressed EC cells were analyzed using the commercial kits. (C and D) Reverse transcription-quantitative PCR and western blotting were used to detect the expression of SLC1A5 and GLS in EC cells. *P

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