STIL Antibody - #DF13333

(3)  
Product: STIL Antibody
Catalog: DF13333
Description: Rabbit polyclonal antibody to STIL
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 142kDa; 143kD(Calculated).
Uniprot: Q15468
RRID: AB_2846352

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(88%)
Clonality:
Polyclonal
Specificity:
STIL Antibody detects endogenous levels of total STIL.
RRID:
AB_2846352
Cite Format: Affinity Biosciences Cat# DF13333, RRID:AB_2846352.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp686O09161; MCPH7; OTTHUMP00000009566; SCL interrupting locus protein; SCL-interrupting locus protein; SCL/TAL1 interrupting locus; SIL; STIL; STIL_HUMAN; TAL 1 interrupting locus protein; TAL-1-interrupting locus protein;

Immunogens

Immunogen:
Uniprot:

Q15468(STIL_HUMAN) >>Visit HPA database.

Gene(ID):
STIL(6491)
Expression:
Q15468 STIL_HUMAN:

Expressed in all hematopoietic tissues and cell lines. Highly expressed in a variety of tumors characterized by increased mitotic activity with highest expression in lung cancer.

Sequence:
MEPIYPFARPQMNTRFPSSRMVPFHFPPSKCALWNPTPTGDFIYLHLSYYRNPKLVVTEKTIRLAYRHAKQNKKNSSCFLLGSLTADEDEEGVTLTVDRFDPGREVPECLEITPTASLPGDFLIPCKVHTQELCSREMIVHSVDDFSSALKALQCHICSKDSLDCGKLLSLRVHITSRESLDSVEFDLHWAAVTLANNFKCTPVKPIPIIPTALARNLSSNLNISQVQGTYKYGYLTMDETRKLLLLLESDPKVYSLPLVGIWLSGITHIYSPQVWACCLRYIFNSSVQERVFSESGNFIIVLYSMTHKEPEFYECFPCDGKIPDFRFQLLTSKETLHLFKNVEPPDKNPIRCELSAESQNAETEFFSKASKNFSIKRSSQKLSSGKMPIHDHDSGVEDEDFSPRPIPSPHPVSQKISKIQPSVPELSLVLDGNFIESNPLPTPLEMVNNENPPLINHLEHLKPLQPQLYDEKHSPEVEAGEPSLRGIPNQLNQDKPALLRHCKVRQPPAYKKGNPHTRNSIKPSSHNGPSHDIFEKLQTVSAGNVQNEEYPIRPSTLNSRQSSLAPQSQPHDFVFSPHNSGRPMELQIPTPPLPSYCSTNVCRCCQHHSHIQYSPLNSWQGANTVGSIQDVQSEALQKHSLFHPSGCPALYCNAFCSSSSPIALRPQGDMGSCSPHSNIEPSPVARPPSHMDLCNPQPCTVCMHTPKTESDNGMMGLSPDAYRFLTEQDRQLRLLQAQIQRLLEAQSLMPCSPKTTAVEDTVQAGRQMELVSVEAQSSPGLHMRKGVSIAVSTGASLFWNAAGEDQEPDSQMKQDDTKISSEDMNFSVDINNEVTSLPGSASSLKAVDIPSFEESNIAVEEEFNQPLSVSNSSLVVRKEPDVPVFFPSGQLAESVSMCLQTGPTGGASNNSETSEEPKIEHVMQPLLHQPSDNQKIYQDLLGQVNHLLNSSSKETEQPSTKAVIISHECTRTQNVYHTKKKTHHSRLVDKDCVLNATLKQLRSLGVKIDSPTKVKKNAHNVDHASVLACISPEAVISGLNCMSFANVGMSGLSPNGVDLSMEANAIALKYLNENQLSQLSVTRSNQNNCDPFSLLHINTDRSTVGLSLISPNNMSFATKKYMKRYGLLQSSDNSEDEEEPPDNADSKSEYLLNQNLRSIPEQLGGQKEPSKNDHEIINCSNCESVGTNADTPVLRNITNEVLQTKAKQQLTEKPAFLVKNLKPSPAVNLRTGKAEFTQHPEKENEGDITIFPESLQPSETLKQMNSMNSVGTFLDVKRLRQLPKLF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Rabbit
100
Sheep
88
Dog
88
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15468 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K54 Ubiquitination
K60 Ubiquitination
T94 Phosphorylation
T96 Phosphorylation
K127 Ubiquitination
S147 Phosphorylation
S148 Phosphorylation
K151 Ubiquitination
S162 Phosphorylation
K243 Ubiquitination
S272 Phosphorylation
K334 Ubiquitination
T336 Phosphorylation
K341 Ubiquitination
K348 Ubiquitination
K369 Ubiquitination
K372 Ubiquitination
S375 Phosphorylation
S395 Phosphorylation
S403 Phosphorylation
S409 Phosphorylation
K416 Ubiquitination
Y470 Phosphorylation
S475 Phosphorylation
K496 Ubiquitination
K512 Acetylation
S521 Phosphorylation
K523 Ubiquitination
T591 Phosphorylation
S719 Phosphorylation
S753 Phosphorylation
K755 Ubiquitination
S779 Phosphorylation
S852 Phosphorylation
S869 Phosphorylation
S871 Phosphorylation
S873 Phosphorylation
S874 Phosphorylation
K936 Ubiquitination
Y938 Phosphorylation
K954 Ubiquitination
K962 Ubiquitination
K991 Ubiquitination
K1000 Ubiquitination
S1004 Phosphorylation
K1008 Ubiquitination
S1011 Phosphorylation
K1014 Ubiquitination
S1061 Phosphorylation
S1108 Phosphorylation P53350 (PLK1)
S1111 Phosphorylation
S1116 Phosphorylation P53350 (PLK1)
T1119 Phosphorylation
K1120 Ubiquitination
S1132 Phosphorylation
S1135 Phosphorylation
K1168 Ubiquitination
K1206 Ubiquitination
K1214 Ubiquitination
K1223 Ubiquitination
S1225 Phosphorylation
K1234 Ubiquitination
K1243 Ubiquitination
T1250 Phosphorylation

Research Backgrounds

Function:

Immediate-early gene. Plays an important role in embryonic development as well as in cellular growth and proliferation; its long-term silencing affects cell survival and cell cycle distribution as well as decreases CDK1 activity correlated with reduced phosphorylation of CDK1. Plays a role as a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1. Plays an important role in the regulation of centriole duplication. Required for the onset of procentriole formation and proper mitotic progression. During procentriole formation, is essential for the correct loading of SASS6 and CENPJ to the base of the procentriole to initiate procentriole assembly.

PTMs:

Ubiquitinated.

Phosphorylated following the activation of the mitotic checkpoint.

Subcellular Location:

Cytoplasm>Cytosol. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all hematopoietic tissues and cell lines. Highly expressed in a variety of tumors characterized by increased mitotic activity with highest expression in lung cancer.

Subunit Structure:

Homodimer. Interacts with PIN1 via its WW domain. This interaction is dependent on STIL mitotic phosphorylation (By similarity). Interacts with CENPJ. Interacts with RBM14 and this interaction interferes with the interaction of STIL with CENPJ. Forms a complex with CENPJ and SASS6.

Restrictive clause

 

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