TFII I Antibody | Affinity Biosciences

1/3

Western blot analysis of extracts from Hela cells , using TFII I Antibody.

Western blot analysis of extracts from Rat heart, using TFII I Antibody.

DF13330 at 1/100 staining Human prostate cancer by IHC-P.

Product:	TFII I Antibody
Catalog:	DF13330
Description:	Rabbit polyclonal antibody to TFII I
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	112kDa; 112kD(Calculated).
Uniprot:	P78347
RRID:	AB_2846349

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

TFII I Antibody detects endogenous levels of total TFII I.

RRID:

AB_2846349
Cite Format: Affinity Biosciences Cat# DF13330, RRID:AB_2846349.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BAP 135; BAP-135; BAP135; Bruton tyrosine kinase associated protein 135; Bruton tyrosine kinase-associated protein 135; BTK associated protein 135; BTK associated protein 135kD; BTK associated protein; BTK-associated protein 135; BTKAP 1; BTKAP1; DIWS; FLJ38776; FLJ56355; General transcription factor II i; General transcription factor II-I; General transcription factor IIi; GTF 2I; Gtf2i; GTF2I_HUMAN; GTFII I; GTFII-I; IB 291; IB291; SPIN; SRF Phox 1 interacting protein; SRF Phox1 interacting protein; SRF-Phox1-interacting protein; TFII-I; Transcription factor II I; WBS; WBSCR 6; WBSCR6; Williams Beuren syndrome chromosome region 6; Williams Beuren syndrome chromosome region 6 protein; Williams-Beuren syndrome chromosomal region 6 protein;

Immunogens

Immunogen:

Uniprot:

P78347(GTF2I_HUMAN) >>Visit HPA database.

Gene(ID):

GTF2I(2969)

Expression:

P78347 GTF2I_HUMAN:

Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues.

Sequence:

P78347 GTF2I_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAQVAMSTLPVEDEESSESRMVVTFLMSALESMCKELAKSKAEVACIAVYETDVFVVGTERGRAFVNTRKDFQKDFVKYCVEEEEKAAEMHKMKSTTQANRMSVDAVEIETLRKTVEDYFCFCYGKALGKSTVVPVPYEKMLRDQSAVVVQGLPEGVAFKHPENYDLATLKWILENKAGISFIIKRPFLEPKKHVGGRVMVTDADRSILSPGGSCGPIKVKTEPTEDSGISLEMAAVTVKEESEDPDYYQYNIQAGPSETDDVDEKQPLSKPLQGSHHSSEGNEGTEMEVPAEDSTQHVPSETSEDPEVEVTIEDDDYSPPSKRPKANELPQPPVPEPANAGKRKVREFNFEKWNARITDLRKQVEELFERKYAQAIKAKGPVTIPYPLFQSHVEDLYVEGLPEGIPFRRPSTYGIPRLERILLAKERIRFVIKKHELLNSTREDLQLDKPASGVKEEWYARITKLRKMVDQLFCKKFAEALGSTEAKAVPYQKFEAHPNDLYVEGLPENIPFRSPSWYGIPRLEKIIQVGNRIKFVIKRPELLTHSTTEVTQPRTNTPVKEDWNVRITKLRKQVEEIFNLKFAQALGLTEAVKVPYPVFESNPEFLYVEGLPEGIPFRSPTWFGIPRLERIVRGSNKIKFVVKKPELVISYLPPGMASKINTKALQSPKRPRSPGSNSKVPEIEVTVEGPNNNNPQTSAVRTPTQTNGSNVPFKPRGREFSFEAWNAKITDLKQKVENLFNEKCGEALGLKQAVKVPFALFESFPEDFYVEGLPEGVPFRRPSTFGIPRLEKILRNKAKIKFIIKKPEMFETAIKESTSSKSPPRKINSSPNVNTTASGVEDLNIIQVTIPDDDNERLSKVEKARQLREQVNDLFSRKFGEAIGMGFPVKVPYRKITINPGCVVVDGMPPGVSFKAPSYLEISSMRRILDSAEFIKFTVIRPFPGLVINNQLVDQSESEGPVIQESAEPSQLEVPATEEIKETDGSSQIKQEPDPTW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P78347 As Substrate

P78347

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
T8	Phosphorylation		Uniprot
S16	Phosphorylation		Uniprot
S19	Phosphorylation		Uniprot
K74	Ubiquitination		Uniprot
K78	Ubiquitination		Uniprot
K86	Sumoylation		Uniprot
K94	Sumoylation		Uniprot
S95	Phosphorylation		Uniprot
R101	Methylation		Uniprot
S103	Phosphorylation		Uniprot
T111	Phosphorylation		Uniprot
C121	S-Nitrosylation		Uniprot
C123	S-Nitrosylation		Uniprot
K130	Sumoylation		Uniprot
K130	Ubiquitination		Uniprot
K140	Sumoylation		Uniprot
K140	Ubiquitination		Uniprot
R143	Methylation		Uniprot
S146	Phosphorylation		Uniprot
K160	Ubiquitination		Uniprot
K171	Ubiquitination		Uniprot
K177	Sumoylation		Uniprot
S181	Phosphorylation		Uniprot
T202	Phosphorylation		Uniprot
R206	Methylation		Uniprot
S207	Phosphorylation		Uniprot
S210	Phosphorylation		Uniprot
S214	Phosphorylation		Uniprot
K219	Methylation		Uniprot
K219	Ubiquitination		Uniprot
K221	Sumoylation		Uniprot
T238	Phosphorylation		Uniprot
K240	Sumoylation		Uniprot
Y248	Phosphorylation	P12931 (SRC) , Q06187 (BTK) , O60674 (JAK2)	Uniprot
Y249	Phosphorylation	P12931 (SRC)	Uniprot
Y318	Phosphorylation		Uniprot
S319	Phosphorylation		Uniprot
K326	Sumoylation		Uniprot
K343	Acetylation		Uniprot
K343	Methylation		Uniprot
K343	Sumoylation		Uniprot
K353	Sumoylation		Uniprot
K353	Ubiquitination		Uniprot
K363	Sumoylation		Uniprot
K363	Ubiquitination		Uniprot
K372	Sumoylation		Uniprot
K372	Ubiquitination		Uniprot
K378	Sumoylation		Uniprot
K378	Ubiquitination		Uniprot
K380	Ubiquitination		Uniprot
Y398	Phosphorylation	Q06187 (BTK)	Uniprot
S412	Phosphorylation	Q13976 (PRKG1)	Uniprot
T413	Phosphorylation		Uniprot
K435	Sumoylation		Uniprot
K435	Ubiquitination		Uniprot
K450	Acetylation		Uniprot
K450	Sumoylation		Uniprot
K450	Ubiquitination		Uniprot
S453	Phosphorylation		Uniprot
K456	Methylation		Uniprot
K456	Sumoylation		Uniprot
K456	Ubiquitination		Uniprot
Y460	Phosphorylation		Uniprot
C475	S-Nitrosylation		Uniprot
K476	Ubiquitination		Uniprot
K477	Sumoylation		Uniprot
K477	Ubiquitination		Uniprot
K488	Sumoylation		Uniprot
K488	Ubiquitination		Uniprot
K494	Sumoylation		Uniprot
K494	Ubiquitination		Uniprot
Y503	Phosphorylation	Q06187 (BTK)	Uniprot
S515	Phosphorylation		Uniprot
S517	Phosphorylation		Uniprot
Y519	Phosphorylation		Uniprot
K526	Sumoylation		Uniprot
K526	Ubiquitination		Uniprot
K539	Ubiquitination		Uniprot
S547	Phosphorylation		Uniprot
T556	Phosphorylation	P24941 (CDK2)	Uniprot
T558	Phosphorylation	P24941 (CDK2)	Uniprot
K561	Acetylation		Uniprot
K561	Sumoylation		Uniprot
K561	Ubiquitination		Uniprot
K573	Ubiquitination		Uniprot
S620	Phosphorylation		Uniprot
K640	Sumoylation		Uniprot
K645	Sumoylation		Uniprot
S659	Phosphorylation		Uniprot
K660	Ubiquitination		Uniprot
T663	Phosphorylation		Uniprot
K664	Sumoylation		Uniprot
K664	Ubiquitination		Uniprot
S668	Phosphorylation	P28482 (MAPK1) , P27361 (MAPK3)	Uniprot
S674	Phosphorylation	P27361 (MAPK3) , P28482 (MAPK1)	Uniprot
S677	Phosphorylation		Uniprot
S679	Phosphorylation		Uniprot
T687	Phosphorylation		Uniprot
T698	Phosphorylation		Uniprot
S699	Phosphorylation		Uniprot
T703	Phosphorylation		Uniprot
T705	Phosphorylation		Uniprot
K715	Acetylation		Uniprot
K715	Methylation		Uniprot
K715	Sumoylation		Uniprot
K715	Ubiquitination		Uniprot
S722	Phosphorylation		Uniprot
K729	Ubiquitination		Uniprot
K734	Sumoylation		Uniprot
K736	Sumoylation		Uniprot
K736	Ubiquitination		Uniprot
K744	Sumoylation		Uniprot
K744	Ubiquitination		Uniprot
K752	Sumoylation		Uniprot
K752	Ubiquitination		Uniprot
K756	Ubiquitination		Uniprot
Y770	Phosphorylation		Uniprot
S784	Phosphorylation	Q13976 (PRKG1)	Uniprot
T785	Phosphorylation		Uniprot
K806	Ubiquitination		Uniprot
K807	Sumoylation		Uniprot
K807	Ubiquitination		Uniprot
K816	Sumoylation		Uniprot
S818	Phosphorylation		Uniprot
S820	Phosphorylation		Uniprot
S821	Phosphorylation		Uniprot
S823	Phosphorylation		Uniprot
K827	Ubiquitination		Uniprot
S830	Phosphorylation		Uniprot
S831	Phosphorylation		Uniprot
T836	Phosphorylation		Uniprot
T837	Phosphorylation		Uniprot
S839	Phosphorylation		Uniprot
T850	Phosphorylation		Uniprot
K861	Sumoylation		Uniprot
S877	Phosphorylation		Uniprot
K879	Sumoylation		Uniprot
K879	Ubiquitination		Uniprot
K891	Sumoylation		Uniprot
K891	Ubiquitination		Uniprot
Y894	Phosphorylation		Uniprot
K896	Sumoylation		Uniprot
K896	Ubiquitination		Uniprot
Y920	Phosphorylation		Uniprot
S925	Phosphorylation		Uniprot
R927	Methylation		Uniprot
S932	Phosphorylation		Uniprot
S971	Phosphorylation		Uniprot
K982	Sumoylation		Uniprot
S988	Phosphorylation		Uniprot
K991	Sumoylation		Uniprot
K991	Ubiquitination		Uniprot

Research Backgrounds

P78347_GTF2I_HUMAN

Function:

Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.

PTMs:

Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation.

Sumoylated.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Colocalizes with BTK in the cytoplasm.

Tissue Specificity:

Subunit Structure:

Homodimer (Potential). Interacts with SRF and PHOX1. Binds a pyrimidine-rich initiator (Inr) and a recognition site (E-box) for upstream stimulatory factor 1 (USF1). Associates with the PH domain of Bruton's tyrosine kinase (BTK). May be a component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, KDM1A, RCOR1/CoREST, PHF21A/BHC80, ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Interacts with BTK and ARID3A. Interacts with isoform beta of PRKG1.

Family&Domains:

Belongs to the TFII-I family.

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Genetic Information Processing > Transcription > Basal transcription factors.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

TFII I Antibody - #DF13330