Prolactin Receptor Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from A431 cells, using Prolactin Receptor Antibody.

Product:	Prolactin Receptor Antibody
Catalog:	DF13327
Description:	Rabbit polyclonal antibody to Prolactin Receptor
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Sheep, Rabbit
Mol.Wt.:	69kDa; 70kD(Calculated).
Uniprot:	P16471
RRID:	AB_2846346

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(86%), Sheep(86%), Rabbit(88%)

Clonality:

Polyclonal

Specificity:

Prolactin Receptor Antibody detects endogenous levels of total Prolactin Receptor.

RRID:

AB_2846346
Cite Format: Affinity Biosciences Cat# DF13327, RRID:AB_2846346.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AI987712; CLONE SPM213; CPRLP; Delta 4-delta 7/11 truncated prolactin receptor; Delta 4-SF1b truncated prolactin receptor; HPRL; hPRL receptor; hPRLrI; Lactogen receptor; MFAB; MGC105486; OPR; OTTHUMP00000115998; Pr-1; Pr-3; PRL R; PRL-R; PRLR; Prlr-rs1; PRLR_HUMAN; Prolactin receptor a; Prolactin receptor; Prolactin receptor delta 7/11; RATPRLR; Secreted prolactin binding protein; Truncated testis-specific box 1-C prolactin receptor; wu:fj65c07;

Immunogens

Immunogen:

Uniprot:

P16471(PRLR_HUMAN) >>Visit HPA database.

Gene(ID):

PRLR(5618)

Expression:

P16471 PRLR_HUMAN:

Expressed in breast, placenta, kidney, liver and pancreas.

Sequence:

P16471 PRLR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKENVASATVFTLLLFLNTCLLNGQLPPGKPEIFKCRSPNKETFTCWWRPGTDGGLPTNYSLTYHREGETLMHECPDYITGGPNSCHFGKQYTSMWRTYIMMVNATNQMGSSFSDELYVDVTYIVQPDPPLELAVEVKQPEDRKPYLWIKWSPPTLIDLKTGWFTLLYEIRLKPEKAAEWEIHFAGQQTEFKILSLHPGQKYLVQVRCKPDHGYWSAWSPATFIQIPSDFTMNDTTVWISVAVLSAVICLIIVWAVALKGYSMVTCIFPPVPGPKIKGFDAHLLEKGKSEELLSALGCQDFPPTSDYEDLLVEYLEVDDSEDQHLMSVHSKEHPSQGMKPTYLDPDTDSGRGSCDSPSLLSEKCEEPQANPSTFYDPEVIEKPENPETTHTWDPQCISMEGKIPYFHAGGSKCSTWPLPQPSQHNPRSSYHNITDVCELAVGPAGAPATLLNEAGKDALKSSQTIKSREEGKATQQREVESFHSETDQDTPWLLPQEKTPFGSAKPLDYVEIHKVNKDGALSLLPKQRENSGKPKKPGTPENNKEYAKVSGVMDNNILVLVPDPHAKNVACFEESAKEAPPSLEQNQAEKALANFTATSSKCRLQLGGLDYLDPACFTHSFH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Bovine

Sheep

Pig

Horse

Xenopus

Chicken

Dog

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16471 As Substrate

P16471

Site	PTM Type	Enzyme	Source
S195	Phosphorylation		Uniprot
Y202	Phosphorylation		Uniprot
S349	Phosphorylation	P49841 (GSK3B)	Uniprot
S356	Phosphorylation		Uniprot
S358	Phosphorylation		Uniprot
S361	Phosphorylation		Uniprot
T391	Phosphorylation		Uniprot
T415	Phosphorylation		Uniprot
Y509	Phosphorylation		Uniprot
S522	Phosphorylation		Uniprot
S600	Phosphorylation		Uniprot
Y611	Phosphorylation		Uniprot

Research Backgrounds

P16471_PRLR_HUMAN

Function:

This is a receptor for the anterior pituitary hormone prolactin (PRL). Acts as a prosurvival factor for spermatozoa by inhibiting sperm capacitation through suppression of SRC kinase activation and stimulation of AKT. Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Secreted.

Tissue Specificity:

Expressed in breast, placenta, kidney, liver and pancreas.

Subunit Structure:

Homodimer upon hormone binding. Interacts with SMARCA1. Interacts with GH1. Interacts with CSH. Interacts with NEK3 and VAV2 and this interaction is prolactin-dependent.

Family&Domains:

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Belongs to the type I cytokine receptor family. Type 1 subfamily.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Prolactin signaling pathway. (View pathway)

References

1). Freeze-dried powder of daylily bud improves bromocriptine-induced lactation disorder in rats via JAK2/STAT5 pathway. Journal of Ethnopharmacology, 2023 (PubMed: 37120059) [IF=5.4]

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Prolactin Receptor Antibody - #DF13327