Calcium Pump PMCA4 ATPase Antibody - #DF13326

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Product: Calcium Pump PMCA4 ATPase Antibody
Catalog: DF13326
Description: Rabbit polyclonal antibody to Calcium Pump PMCA4 ATPase
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 137kDa; 138kD(Calculated).
Uniprot: P23634
RRID: AB_2846345

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(88%), Horse(88%), Rabbit(88%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Calcium Pump PMCA4 ATPase Antibody detects endogenous levels of total Calcium Pump PMCA4 ATPase.
RRID:
AB_2846345
Cite Format: Affinity Biosciences Cat# DF13326, RRID:AB_2846345.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AT2B4_HUMAN; ATP2B2; Atp2b4; ATPase Ca++ transporting plasma membrane 4; Cation transporting ATPase; DKFZp686G08106; DKFZp686M088; Matrix remodelling associated 1; Matrix remodelling associated protein 1; Matrix-remodeling-associated protein 1; MXRA 1; MXRA1; OTTHUMP00000034088; OTTHUMP00000034089; Plasma membrane calcium ATPase 4; Plasma membrane calcium ATPase isoform 4; Plasma membrane calcium pump isoform 4; Plasma membrane calcium transporting ATPase 4; Plasma membrane calcium-transporting ATPase 4; PMCA 4; PMCA4; PMCA4b; PMCA4x; Sarcolemmal calcium pump;

Immunogens

Immunogen:
Uniprot:

P23634(AT2B4_HUMAN) >>Visit HPA database.

Gene(ID):
ATP2B4(493)
Expression:
P23634 AT2B4_HUMAN:

Isoform XB is the most abundant isoform and is expressed ubiquitously. Isoforms containing segment Z have only been detected in heart, while isoforms containing segment a have been found in heart, stomach and brain cortex.

Sequence:
MTNPSDRVLPANSMAESREGDFGCTVMELRKLMELRSRDALTQINVHYGGVQNLCSRLKTSPVEGLSGNPADLEKRRQVFGHNVIPPKKPKTFLELVWEALQDVTLIILEIAAIISLVLSFYRPAGEENELCGQVATTPEDENEAQAGWIEGAAILFSVIIVVLVTAFNDWSKEKQFRGLQCRIEQEQKFSIIRNGQLIQLPVAEIVVGDIAQVKYGDLLPADGILIQGNDLKIDESSLTGESDHVKKSLDKDPMLLSGTHVMEGSGRMVVTAVGVNSQTGIILTLLGVNEDDEGEKKKKGKKQGVPENRNKAKTQDGVALEIQPLNSQEGIDNEEKDKKAVKVPKKEKSVLQGKLTRLAVQIGKAGLLMSALTVFILILYFVIDNFVINRRPWLPECTPIYIQYFVKFFIIGITVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTMNRMTVVQAYIGGIHYRQIPSPDVFLPKVLDLIVNGISINSAYTSKILPPEKEGGLPRQVGNKTECALLGFVTDLKQDYQAVRNEVPEEKLYKVYTFNSVRKSMSTVIRNPNGGFRMYSKGASEIILRKCNRILDRKGEAVPFKNKDRDDMVRTVIEPMACDGLRTICIAYRDFDDTEPSWDNENEILTELTCIAVVGIEDPVRPEVPDAIAKCKQAGITVRMVTGDNINTARAIATKCGILTPGDDFLCLEGKEFNRLIRNEKGEVEQEKLDKIWPKLRVLARSSPTDKHTLVKGIIDSTVGEHRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTESLLKRRPYGRNKPLISRTMMKNILGHAFYQLIVIFILVFAGEKFFDIDSGRKAPLHSPPSQHYTIVFNTFVLMQLFNEINSRKIHGEKNVFSGIYRNIIFCSVVLGTFICQIFIVEFGGKPFSCTSLSLSQWLWCLFIGIGELLWGQFISAIPTRSLKFLKEAGHGTTKEEITKDAEGLDEIDHAEMELRRGQILWFRGLNRIQTQIDVINTFQTGASFKGVLRRQNMGQHLDVKLVPSSSYIKVVKAFHSSLHESIQKPYNQKSIHSFMTHPEFAIEEELPRTPLLDEEEEENPDKASKFGTRVLLLDGEVTPYANTNNNAVDCNQVQLPQSDSSLQSLETSV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Horse
88
Bovine
88
Rabbit
88
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23634 As Substrate

Site PTM Type Enzyme
S13 Phosphorylation
S17 Phosphorylation
K31 Ubiquitination
K59 Ubiquitination
S67 Phosphorylation
K75 Ubiquitination
K189 Ubiquitination
Y216 Phosphorylation
K233 Ubiquitination
S238 Phosphorylation
K247 Ubiquitination
K252 Ubiquitination
K314 Ubiquitination
S328 Phosphorylation
K337 Ubiquitination
K349 Ubiquitination
K355 Ubiquitination
T415 Phosphorylation
Y435 Phosphorylation
S436 Phosphorylation
K442 Ubiquitination
S464 Phosphorylation
K523 Ubiquitination
C537 S-Nitrosylation
K547 Ubiquitination
K561 Ubiquitination
K564 Ubiquitination
S570 Phosphorylation
R572 Methylation
S576 Phosphorylation
T577 Phosphorylation
K608 Ubiquitination
K615 Ubiquitination
C632 S-Nitrosylation
K686 Ubiquitination
T691 Phosphorylation
K709 Ubiquitination
K725 Ubiquitination
K735 Ubiquitination
K742 Ubiquitination
K745 Ubiquitination
S757 Phosphorylation
T759 Phosphorylation
K761 Ubiquitination
T763 Phosphorylation
S771 Phosphorylation
T783 Phosphorylation
T787 Phosphorylation
K794 Ubiquitination
Y905 Phosphorylation
K909 Ubiquitination
K1055 Ubiquitination
K1071 Ubiquitination
S1115 Phosphorylation
K1132 Ubiquitination
K1144 Ubiquitination
S1149 Phosphorylation
S1153 Phosphorylation
K1156 Ubiquitination
Y1158 Phosphorylation
K1161 Ubiquitination
S1162 Phosphorylation
S1165 Phosphorylation
T1168 Phosphorylation
T1181 Phosphorylation
K1194 Ubiquitination
K1197 Ubiquitination
Y1212 Phosphorylation

Research Backgrounds

Function:

Calcium/calmodulin-regulated and magnesium-dependent enzyme that catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. By regulating sperm cell calcium homeostasis, may play a role in sperm motility (By similarity).

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cell projection>Cilium>Flagellum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform XB is the most abundant isoform and is expressed ubiquitously. Isoforms containing segment Z have only been detected in heart, while isoforms containing segment a have been found in heart, stomach and brain cortex.

Subunit Structure:

Interacts with PDZD11. Interacts with SLC35G1 and STIM1. Interacts with calmodulin.

Family&Domains:

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Pancreatic secretion.

References

1). Neochlorogenic acid anchors MCU-based calcium overload for cancer therapy. Food & Function, 2021 (PubMed: 34672304) [IF=6.1]

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