SNAP23 Antibody - #DF13314

(1)   (8)  
Product: SNAP23 Antibody
Catalog: DF13314
Description: Rabbit polyclonal antibody to SNAP23
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 23kDa, (isoform)19 kDa; 23kD(Calculated).
Uniprot: O00161
RRID: AB_2846333

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
SNAP23 Antibody detects endogenous levels of total SNAP23.
RRID:
AB_2846333
Cite Format: Affinity Biosciences Cat# DF13314, RRID:AB_2846333.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HsT17016; LS-B8340; SNAP 23; SNAP-23; SNAP23; SNAP23A; SNAP23B; SNP23_HUMAN; Synaptosomal associated protein 23; Synaptosomal associated protein 23kDa; Synaptosomal associated protein; Synaptosomal-associated protein 23; Vesicle membrane fusion protein SNAP 23; Vesicle membrane fusion protein SNAP23; Vesicle-membrane fusion protein SNAP-23;

Immunogens

Immunogen:
Uniprot:

O00161(SNP23_HUMAN) >>Visit HPA database.

Gene(ID):
SNAP23(8773)
Expression:
O00161 SNP23_HUMAN:

Ubiquitous. Highest levels where found in placenta.

Sequence:
MDNLSSEEIQQRAHQITDESLESTRRILGLAIESQDAGIKTITMLDEQKEQLNRIEEGLDQINKDMRETEKTLTELNKCCGLCVCPCNRTKNFESGKAYKTTWGDGGENSPCNVVSKQPGPVTNGQLQQPTTGAASGGYIKRITNDAREDEMEENLTQVGSILGNLKDMALNIGNEIDAQNPQIKRITDKADTNRDRIDIANARAKKLIDS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
83
Xenopus
57
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00161 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S5 Phosphorylation
S6 Phosphorylation
T17 Phosphorylation
S20 Phosphorylation
S23 Phosphorylation P17252 (PRKCA)
T24 Phosphorylation P17252 (PRKCA)
S34 Phosphorylation
K40 Ubiquitination
K49 Ubiquitination
K64 Ubiquitination
K71 Ubiquitination
T72 Phosphorylation
K91 Ubiquitination
S95 Phosphorylation P14618 (PKM)
K97 Ubiquitination
Y99 Phosphorylation
K100 Ubiquitination
T102 Phosphorylation
S110 Phosphorylation
C112 S-Nitrosylation
K117 Ubiquitination
S136 Phosphorylation
Y139 Phosphorylation
K141 Ubiquitination
T144 Phosphorylation
T157 Phosphorylation
S161 Phosphorylation P17252 (PRKCA)
K185 Ubiquitination
K190 Ubiquitination

Research Backgrounds

Function:

Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.

Subcellular Location:

Cell membrane>Peripheral membrane protein. Cell membrane>Lipid-anchor. Cell junction>Synapse>Synaptosome.
Note: Mainly localized to the plasma membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Highest levels where found in placenta.

Subunit Structure:

Homotetramer (via coiled-coil domain), also forms heterotetramers with STX4 and VAMP3. Found in a complex with VAMP8 and STX1A. Found in a complex with VAMP8 and STX4 in pancreas (By similarity). Interacts simultaneously with SNAPIN and SYN4 (By similarity). Interacts with STX1A (By similarity). Interacts with STX12 (By similarity). Interacts tightly to multiple syntaxins and synaptobrevins/VAMPs (By similarity). Interacts with ZDHHC13 (via ANK repeats) (By similarity). Interacts with ZDHHC17 (via ANK repeats).

Family&Domains:

Belongs to the SNAP-25 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

References

1). Obstruction of the Tear Drainage Altered Lacrimal Gland Structure and Function. Investigative Ophthalmology & Visual Science, 2023 (PubMed: 37440262) [IF=4.4]

Application: IF/ICC    Species: Mouse    Sample:

Figure 7. Altered expression and localization of proteins relevant to lacrimal gland fusion and secretion. (A) Rab3d immunofluorescence was abundant in the apical lumen side of the acinar cells in the sham LGs (left panels), but uniformly distributed in the STDOB (middle panels) and more basally localized in the Prickle1 mutant acini. The bottom panels are magnified images from the top panels. Apical and basal positions of the acinus lumen are illustrated in the right corner of the first panel. (B) Western blotting demonstrated reduced Rab3d expression in the STDOB and Prickle1 mutant LGs compared with the sham. (C) Vamp8 immunofluorescence showed a similar altered pattern as the Rab3d in the STDOB and Prickle1 mice. (D) Western blotting showed increased LG expression of Vamp8 in the STDOB and Prickle1 mice. (E) Expression of Snap23 in LG acini was also basally shifted and showed increased levels in STDOB and Prickle1 mice compared with the sham (F). The white dotted lines indicate acini boundaries. For Western blotting and quantification, n = 4 LGs/mice.

Application: WB    Species: Mouse    Sample:

Figure 7. Altered expression and localization of proteins relevant to lacrimal gland fusion and secretion. (A) Rab3d immunofluorescence was abundant in the apical lumen side of the acinar cells in the sham LGs (left panels), but uniformly distributed in the STDOB (middle panels) and more basally localized in the Prickle1 mutant acini. The bottom panels are magnified images from the top panels. Apical and basal positions of the acinus lumen are illustrated in the right corner of the first panel. (B) Western blotting demonstrated reduced Rab3d expression in the STDOB and Prickle1 mutant LGs compared with the sham. (C) Vamp8 immunofluorescence showed a similar altered pattern as the Rab3d in the STDOB and Prickle1 mice. (D) Western blotting showed increased LG expression of Vamp8 in the STDOB and Prickle1 mice. (E) Expression of Snap23 in LG acini was also basally shifted and showed increased levels in STDOB and Prickle1 mice compared with the sham (F). The white dotted lines indicate acini boundaries. For Western blotting and quantification, n = 4 LGs/mice.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.