Phospho-HDAC6 (Ser22) Antibody | Affinity Biosciences

IHC
IF/ICC
pElisa
Cites

1/6

AF3485 at 1/100 staining human testis tissue sections by IHC-P.

AF3485 staining HepG2 cells by ICC/IF.

AF3485 staining Hela cells(4h of LPS treatment) by IF/ICC.

AF3485 staining Hela cells(Etoposide 25μm 5h) by IF/ICC.

peptide-ELISA analysis of AF3485.

Supplementary Fig.

Product:	Phospho-HDAC6 (Ser22) Antibody
Catalog:	AF3485
Description:	Rabbit polyclonal antibody to Phospho-HDAC6 (Ser22)
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	131kDa; 131kD(Calculated).
Uniprot:	Q9UBN7
RRID:	AB_2834923

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Data Sheet

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WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

Phospho-HDAC6 (Ser22) Antibody detects endogenous levels of HDAC6 only when phosphorylated at Serine 22.

RRID:

AB_2834923
Cite Format: Affinity Biosciences Cat# AF3485, RRID:AB_2834923.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CPBHM; FLJ16239; HD 6; HD6; HDAC 6; HDAC6; HDAC6_HUMAN; Histone deacetylase 6 (HD6); Histone deacetylase 6; JM 21; JM21; KIAA0901; OTTHUMP00000032398; OTTHUMP00000197663; PPP1R90; Protein phosphatase 1 regulatory subunit 90;

Immunogens

Immunogen:

Uniprot:

Q9UBN7(HDAC6_HUMAN) >>Visit HPA database.

Gene(ID):

HDAC6(10013)

Description:

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA.

Sequence:

Q9UBN7 HDAC6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTSTGQDSTTTRQRRSRQNPQSPPQDSSVTSKRNIKKGAVPRSIPNLAEVKKKGKMKKLGQAMEEDLIVGLQGMDLNLEAEALAGTGLVLDEQLNEFHCLWDDSFPEGPERLHAIKEQLIQEGLLDRCVSFQARFAEKEELMLVHSLEYIDLMETTQYMNEGELRVLADTYDSVYLHPNSYSCACLASGSVLRLVDAVLGAEIRNGMAIIRPPGHHAQHSLMDGYCMFNHVAVAARYAQQKHRIRRVLIVDWDVHHGQGTQFTFDQDPSVLYFSIHRYEQGRFWPHLKASNWSTTGFGQGQGYTINVPWNQVGMRDADYIAAFLHVLLPVALEFQPQLVLVAAGFDALQGDPKGEMAATPAGFAQLTHLLMGLAGGKLILSLEGGYNLRALAEGVSASLHTLLGDPCPMLESPGAPCRSAQASVSCALEALEPFWEVLVRSTETVERDNMEEDNVEESEEEGPWEPPVLPILTWPVLQSRTGLVYDQNMMNHCNLWDSHHPEVPQRILRIMCRLEELGLAGRCLTLTPRPATEAELLTCHSAEYVGHLRATEKMKTRELHRESSNFDSIYICPSTFACAQLATGAACRLVEAVLSGEVLNGAAVVRPPGHHAEQDAACGFCFFNSVAVAARHAQTISGHALRILIVDWDVHHGNGTQHMFEDDPSVLYVSLHRYDHGTFFPMGDEGASSQIGRAAGTGFTVNVAWNGPRMGDADYLAAWHRLVLPIAYEFNPELVLVSAGFDAARGDPLGGCQVSPEGYAHLTHLLMGLASGRIILILEGGYNLTSISESMAACTRSLLGDPPPLLTLPRPPLSGALASITETIQVHRRYWRSLRVMKVEDREGPSSSKLVTKKAPQPAKPRLAERMTTREKKVLEAGMGKVTSASFGEESTPGQTNSETAVVALTQDQPSEAATGGATLAQTISEAAIGGAMLGQTTSEEAVGGATPDQTTSEETVGGAILDQTTSEDAVGGATLGQTTSEEAVGGATLAQTTSEAAMEGATLDQTTSEEAPGGTELIQTPLASSTDHQTPPTSPVQGTTPQISPSTLIGSLRTLELGSESQGASESQAPGEENLLGEAAGGQDMADSMLMQGSRGLTDQAIFYAVTPLPWCPHLVAVCPIPAAGLDVTQPCGDCGTIQENWVCLSCYQVYCGRYINGHMLQHHGNSGHPLVLSYIDLSAWCYYCQAYVHHQALLDVKNIAHQNKFGEDMPHPH

PTMs - Q9UBN7 As Substrate

Q9UBN7

Site	PTM Type	Enzyme	Source
S16	Phosphorylation		Uniprot
S22	Phosphorylation	P49841 (GSK3B)	Uniprot
S28	Phosphorylation		Uniprot
T30	Phosphorylation		Uniprot
K32	Ubiquitination		Uniprot
K36	Acetylation		Uniprot
K37	Acetylation		Uniprot
S43	Phosphorylation		Uniprot
K51	Ubiquitination		Uniprot
K116	Ubiquitination		Uniprot
S412	Phosphorylation		Uniprot
S458	Phosphorylation	P68400 (CSNK2A1)	Uniprot
Y570	Phosphorylation	P00533 (EGFR)	Uniprot
S846	Phosphorylation		Uniprot
S847	Phosphorylation		Uniprot
K849	Acetylation		Uniprot
K849	Ubiquitination		Uniprot
K853	Acetylation		Uniprot
K854	Acetylation		Uniprot
K854	Ubiquitination		Uniprot
K860	Ubiquitination		Uniprot
T868	Phosphorylation		Uniprot
K873	Acetylation		Uniprot
K881	Acetylation		Uniprot
T923	Phosphorylation		Uniprot
S925	Phosphorylation		Uniprot
T993	Phosphorylation		Uniprot
S995	Phosphorylation		Uniprot
T1003	Phosphorylation		Uniprot
T1016	Phosphorylation		Uniprot
T1021	Phosphorylation		Uniprot
T1027	Phosphorylation		Uniprot
T1031	Phosphorylation	P27361 (MAPK3) , P28482 (MAPK1)	Uniprot
T1034	Phosphorylation		Uniprot
S1035	Phosphorylation	P27361 (MAPK3)	Uniprot
T1040	Phosphorylation		Uniprot
T1041	Phosphorylation		Uniprot
T1048	Phosphorylation		Uniprot
T1055	Phosphorylation		Uniprot
S1062	Phosphorylation		Uniprot
K1206	Ubiquitination		Uniprot

Research Backgrounds

Q9UBN7_HDAC6_HUMAN

Function:

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. In addition to histones, deacetylates other proteins: plays a central role in microtubule-dependent cell motility by mediating deacetylation of tubulin. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins: when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin-proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome. Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy.

PTMs:

Phosphorylated by AURKA.

Ubiquitinated. Its polyubiquitination however does not lead to its degradation.

Sumoylated in vitro.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton. Nucleus. Perikaryon. Cell projection>Dendrite. Cell projection>Axon.
Note: It is mainly cytoplasmic, where it is associated with microtubules.

Subunit Structure:

Interacts with SIRT2 (via both phosphorylated, unphosphorylated, active or inactive forms); the interaction is necessary for the complex to interact with alpha-tubulin. Under proteasome impairment conditions, interacts with UBD via its histone deacetylase 1 and UBP-type zinc-finger regions. Interacts with BBIP10, CBFA2T3, CYLD, DDIT3/CHOP, ZMYND15, F-actin and HDAC11. Interacts with RIPOR2; this interaction occurs during early myogenic differentiation and prevents HDAC6 to deacetylate tubulin. Interacts with DYSF; this interaction occurs during early myogenic differentiation. Interacts with TPPP; inhibiting the tubulin deacetylase activity of HDAC6. Interacts with DYNLL1.

Family&Domains:

Belongs to the histone deacetylase family. HD type 2 subfamily.

Research Fields

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

References

1). OCT4B-190 protects against ischemic stroke by modulating GSK-3β/HDAC6. EXPERIMENTAL NEUROLOGY, 2019 (PubMed: 30981804) [IF=5.3]

Application: WB Species: mouse Sample: primary neuronal cultures

Supplementary Fig. 7. |The independence of pHDAC6 with GSK-3β kinase activation. Primary cortical neurons were infected with adenovirus expressing OCT4B-190 or control adenovirus for 3 days, and then neurons were subjected to DMSO or AKT inhibitor (AKT-I), 1 h after which the expression of GSK-3β and HDAC6 was examined by western blotting. A. Representative immunoblot of GSK-3βand HDAC6 from primary neuronal cultures.

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Phospho-HDAC6 (Ser22) Antibody - #AF3485