KAT8 Antibody - #DF13309

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Product: KAT8 Antibody
Catalog: DF13309
Description: Rabbit polyclonal antibody to KAT8
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 52kDa; 52kD(Calculated).
Uniprot: Q9H7Z6
RRID: AB_2846328

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
KAT8 Antibody detects endogenous levels of total KAT8.
RRID:
AB_2846328
Cite Format: Affinity Biosciences Cat# DF13309, RRID:AB_2846328.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Histone acetyltransferase KAT8; Histone acetyltransferase MYST1; hMOF; K(lysine) acetyltransferase 8; KAT 8; Lysine acetyltransferase 8; MOF; MOZ; MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1; MYST 1; MYST histone acetyltransferase 1; myst protein 1; MYST-1; MYST1; MYST1_HUMAN; Ortholog of Drosophila males absent on the first (MOF); Probable histone acetyltransferase MYST1; SAS2 and TIP60 protein 1; SAS2; SAS3; TIP60 protein 1; YBF2; YBF2/SAS3; ZC2HC8;

Immunogens

Immunogen:
Uniprot:

Q9H7Z6(KAT8_HUMAN) >>Visit HPA database.

Gene(ID):
KAT8(84148)
Sequence:
MAAQGAAAAVAAGTSGVAGEGEPGPGENAAAEGTAPSPGRVSPPTPARGEPEVTVEIGETYLCRRPDSTWHSAEVIQSRVNDQEGREEFYVHYVGFNRRLDEWVDKNRLALTKTVKDAVQKNSEKYLSELAEQPERKITRNQKRKHDEINHVQKTYAEMDPTTAALEKEHEAITKVKYVDKIHIGNYEIDAWYFSPFPEDYGKQPKLWLCEYCLKYMKYEKSYRFHLGQCQWRQPPGKEIYRKSNISVYEVDGKDHKIYCQNLCLLAKLFLDHKTLYFDVEPFVFYILTEVDRQGAHIVGYFSKEKESPDGNNVACILTLPPYQRRGYGKFLIAFSYELSKLESTVGSPEKPLSDLGKLSYRSYWSWVLLEILRDFRGTLSIKDLSQMTSITQNDIISTLQSLNMVKYWKGQHVICVTPKLVEEHLKSAQYKKPPITVDSVCLKWAPPKHKQVKLSKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H7Z6 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T14 Phosphorylation
S15 Phosphorylation
S37 Phosphorylation
S42 Phosphorylation
Y90 Phosphorylation
K106 Ubiquitination
K113 Acetylation
K113 Ubiquitination
T114 Phosphorylation
K116 Acetylation
K125 Ubiquitination
K154 Ubiquitination
K168 Acetylation
K168 Ubiquitination
T174 Phosphorylation
K175 Ubiquitination
K177 Acetylation
K238 Acetylation
Y241 Phosphorylation
K243 Ubiquitination
K254 Ubiquitination
K257 Ubiquitination
K274 Acetylation Q9H7Z6 (KAT8)
S344 Phosphorylation
S348 Phosphorylation
K351 Acetylation
K351 Ubiquitination
T392 Phosphorylation
K410 Ubiquitination
K427 Ubiquitination
K433 Ubiquitination
K444 Ubiquitination

PTMs - Q9H7Z6 As Enzyme

Substrate Site Source
Q9H7Z6 (KAT8) K274 Uniprot

Research Backgrounds

Function:

Histone acetyltransferase which may be involved in transcriptional activation. May influence the function of ATM. As part of the MSL complex it is involved in acetylation of nucleosomal histone H4 producing specifically H4K16ac. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. That activity is less specific than the one of the MSL complex. Can also acetylate TP53/p53 at 'Lys-120'.

PTMs:

Autoacetylation at Lys-274 is required for binding histone H4 with high affinity and for proper function.

Subcellular Location:

Nucleus. Chromosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of a multisubunit histone acetyltransferase complex (MSL) at least composed of the MOF/KAT8, MSL1/hampin, MSL2L1 and MSL3L1. Interacts with MSL1; the interaction is direct. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MOF/KAT8, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with the chromodomain of MORF4L1/MRG15. Interacts with ATM through the chromodomain. Interacts with KANSL1; the interaction is direct. Interacts with MSL3. Interacts with NELFD (By similarity).

Family&Domains:

Belongs to the MYST (SAS/MOZ) family.

References

1). Attenuation of histone H4 lysine 16 acetylation (H4K16ac) elicits a neuroprotection against ischemic stroke by alleviating the autophagic/lysosomal dysfunction in neurons at the penumbra. BRAIN RESEARCH BULLETIN, 2022 (PubMed: 35351588) [IF=3.8]
2). Histone Acetyltransferase Mof Affects the Progression of DSS-Induced Colitis. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2018 (PubMed: 29975939)

Application: WB    Species: mouse    Sample: colonic

Fig. 1. |Expression of Mof was increased during DSSinduced colitis in mice.(A) qPCR analysis of Mof mRNA expression in colonic tissues of mice after 3.5% DSS treatment for 3 or 7 days. (B)Expression of Mof and H4K16ac detected by western blotting during DSS-induced colitis in mice. GAPDH serves as a loading control. IL17a serves as a positive control protein which has been reported to be changed during DSS-induced colitis

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