FKBP51 Antibody - #DF13305

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Product: FKBP51 Antibody
Catalog: DF13305
Description: Rabbit polyclonal antibody to FKBP51
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 51kDa; 51kD(Calculated).
Uniprot: Q13451
RRID: AB_2846324

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
FKBP51 Antibody detects endogenous levels of total FKBP51.
RRID:
AB_2846324
Cite Format: Affinity Biosciences Cat# DF13305, RRID:AB_2846324.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

51 kDa FK506 binding protein 5; 51 kDa FK506 binding protein; 51 kDa FK506-binding protein; 51 kDa FKBP; 54 kDa progesterone receptor associated immunophilin; 54 kDa progesterone receptor-associated immunophilin; AIG 6; AIG6; Androgen regulated protein 6; Androgen-regulated protein 6; FF1 antigen; FK506 binding protein 5; FK506-binding protein 5; FKBP 5; FKBP 51; FKBP 54; FKBP-5; FKBP-51; FKBP5; FKBP5_HUMAN; FKBP54; HSP90 binding immunophilin; HSP90-binding immunophilin; MGC111006; OTTHUMP00000016268; P54; Peptidyl prolyl cis trans isomerase; Peptidyl-prolyl cis-trans isomerase FKBP5; Peptidylprolyl cis trans isomerase; PPIase; PPIase FKBP5; Ptg 10; Ptg10; Rotamase; T cell FK506 binding protein;

Immunogens

Immunogen:
Uniprot:

Q13451(FKBP5_HUMAN) >>Visit HPA database.

Gene(ID):
FKBP5(2289)
Expression:
Q13451 FKBP5_HUMAN:

Widely expressed, enriched in testis compared to other tissues.

Sequence:
MTTDEGAKNNEESPTATVAEQGEDITSKKDRGVLKIVKRVGNGEETPMIGDKVYVHYKGKLSNGKKFDSSHDRNEPFVFSLGKGQVIKAWDIGVATMKKGEICHLLCKPEYAYGSAGSLPKIPSNATLFFEIELLDFKGEDLFEDGGIIRRTKRKGEGYSNPNEGATVEIHLEGRCGGRMFDCRDVAFTVGEGEDHDIPIGIDKALEKMQREEQCILYLGPRYGFGEAGKPKFGIEPNAELIYEVTLKSFEKAKESWEMDTKEKLEQAAIVKEKGTVYFKGGKYMQAVIQYGKIVSWLEMEYGLSEKESKASESFLLAAFLNLAMCYLKLREYTKAVECCDKALGLDSANEKGLYRRGEAQLLMNEFESAKGDFEKVLEVNPQNKAARLQISMCQKKAKEHNERDRRIYANMFKKFAEQDAKEEANKAMGKKTSEGVTNEKGTDSQAMEEEKPEGHV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
0
Bovine
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13451 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S13 Phosphorylation
T15 Phosphorylation
K28 Acetylation
K28 Ubiquitination
Y54 Phosphorylation
K155 Acetylation
K208 Ubiquitination
Y243 Phosphorylation
K248 Acetylation
K248 Ubiquitination
K274 Ubiquitination
K342 Ubiquitination
K352 Ubiquitination
R357 Methylation
K376 Ubiquitination
K385 Ubiquitination
Y409 Phosphorylation
K414 Acetylation
K415 Acetylation
K422 Sumoylation
K422 Ubiquitination
T433 Phosphorylation
S434 Phosphorylation
K441 Acetylation
K441 Ubiquitination
T443 Phosphorylation
S445 Phosphorylation

Research Backgrounds

Function:

Immunophilin protein with PPIase and co-chaperone activities. Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). Plays a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors maintaining the complex into the cytoplasm when unliganded. Acts as a regulator of Akt/AKT1 activity by promoting the interaction between Akt/AKT1 and PHLPP1, thereby enhancing dephosphorylation and subsequent activation of Akt/AKT1.

PTMs:

Acetylation impairs ability to promote interaction between Akt/AKT1 and PHLPP1. Deacetylation by SIRT7 promotes interaction between Akt/AKT1 and PHLPP1, leading to suppress Akt/AKT1 activation.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed, enriched in testis compared to other tissues.

Subunit Structure:

Part of a heteromultimeric cytoplasmic complex with HSP90AA1, HSPA1A/HSPA1B and steroid receptors. Upon ligand binding dissociates from the complex and FKBP4 takes its place (By similarity). Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP. Interacts with NR3C1 (By similarity). Interacts with Akt/AKT1 and PHLPP1; enhancing dephosphorylation and subsequent activation of Akt/AKT1.

Research Fields

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

References

1). Rosmarinic acid alleviate CORT-induced depressive-like behavior by promoting neurogenesis and regulating BDNF/TrkB/PI3K signaling axis. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38070249) [IF=7.5]

Application: WB    Species: Mouse    Sample:

Fig. 3. Effect of RA on the HPA axis and hippocampus region glucocorticoid-related proteins expression in chronic CORT injection depression model mice. (A) The levels of CORT in the serum (n = 6). (B) The levels of ACTH in the serum (n = 6). (C) The levels of CRH in the serum (n = 6). (D) Representative protein bands of glucocorticoid pathway (FKBP51, SGK-1, p-GR, GR, HSP90) (n = 3) in the hippocampus of mice. Statistical graphs of relative protein expression of FKBP51 (E), ratio of p-GR(Ser211)/GR (F), relative protein expression of SGK-1 (G) and relative protein expression of HSP90 (H). Data were normalized to the control and presented as means ± SEM.
2). Novel immunosuppressive effect of FK506 by upregulation of PD‐L1 via FKBP51 in heart transplantation. SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2022 (PubMed: 35801698) [IF=3.7]

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