Mineralocorticoid Receptor Antibody - #DF13302

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Product: Mineralocorticoid Receptor Antibody
Catalog: DF13302
Description: Rabbit polyclonal antibody to Mineralocorticoid Receptor
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 107kDa; 107kD(Calculated).
Uniprot: P08235
RRID: AB_2846321

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(83%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(88%)
Clonality:
Polyclonal
Specificity:
Mineralocorticoid Receptor Antibody detects endogenous levels of total Mineralocorticoid Receptor.
RRID:
AB_2846321
Cite Format: Affinity Biosciences Cat# DF13302, RRID:AB_2846321.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Aldosterone receptor; MCR; MCR_HUMAN; MGC133092; Mineralocorticoid receptor; MLR; MR; NR3 C2; NR3C2; NR3C2 protein; Nuclear receptor subfamily 3 group C member 2;

Immunogens

Immunogen:
Uniprot:

P08235(MCR_HUMAN) >>Visit HPA database.

Gene(ID):
NR3C2(4306)
Expression:
P08235 MCR_HUMAN:

Ubiquitous. Highly expressed in distal tubules, convoluted tubules and cortical collecting duct in kidney, and in sweat glands. Detected at lower levels in cardiomyocytes, in epidermis and in colon enterocytes.

Sequence:
METKGYHSLPEGLDMERRWGQVSQAVERSSLGPTERTDENNYMEIVNVSCVSGAIPNNSTQGSSKEKQELLPCLQQDNNRPGILTSDIKTELESKELSATVAESMGLYMDSVRDADYSYEQQNQQGSMSPAKIYQNVEQLVKFYKGNGHRPSTLSCVNTPLRSFMSDSGSSVNGGVMRAVVKSPIMCHEKSPSVCSPLNMTSSVCSPAGINSVSSTTASFGSFPVHSPITQGTPLTCSPNVENRGSRSHSPAHASNVGSPLSSPLSSMKSSISSPPSHCSVKSPVSSPNNVTLRSSVSSPANINNSRCSVSSPSNTNNRSTLSSPAASTVGSICSPVNNAFSYTASGTSAGSSTLRDVVPSPDTQEKGAQEVPFPKTEEVESAISNGVTGQLNIVQYIKPEPDGAFSSSCLGGNSKINSDSSFSVPIKQESTKHSCSGTSFKGNPTVNPFPFMDGSYFSFMDDKDYYSLSGILGPPVPGFDGNCEGSGFPVGIKQEPDDGSYYPEASIPSSAIVGVNSGGQSFHYRIGAQGTISLSRSARDQSFQHLSSFPPVNTLVESWKSHGDLSSRRSDGYPVLEYIPENVSSSTLRSVSTGSSRPSKICLVCGDEASGCHYGVVTCGSCKVFFKRAVEGQHNYLCAGRNDCIIDKIRRKNCPACRLQKCLQAGMNLGARKSKKLGKLKGIHEEQPQQQQPPPPPPPPQSPEEGTTYIAPAKEPSVNTALVPQLSTISRALTPSPVMVLENIEPEIVYAGYDSSKPDTAENLLSTLNRLAGKQMIQVVKWAKVLPGFKNLPLEDQITLIQYSWMCLSSFALSWRSYKHTNSQFLYFAPDLVFNEEKMHQSAMYELCQGMHQISLQFVRLQLTFEEYTIMKVLLLLSTIPKDGLKSQAAFEEMRTNYIKELRKMVTKCPNNSGQSWQRFYQLTKLLDSMHDLVSDLLEFCFYTFRESHALKVEFPAMLVEIISDQLPKVESGNAKPLYFHRK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Rabbit
100
Dog
88
Zebrafish
83
Xenopus
75
Pig
0
Sheep
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08235 As Substrate

Site PTM Type Enzyme
T3 Phosphorylation
Y6 Phosphorylation
S8 Phosphorylation
S23 Phosphorylation
K89 Sumoylation
S111 Phosphorylation
S129 Phosphorylation
T159 Phosphorylation
S168 Phosphorylation
S183 Phosphorylation
S196 Phosphorylation
S227 Phosphorylation
S238 Phosphorylation
S248 Phosphorylation
S250 Phosphorylation
S255 Phosphorylation
S259 Phosphorylation
S262 Phosphorylation
S263 Phosphorylation
S274 Phosphorylation
S283 Phosphorylation
S287 Phosphorylation
S299 Phosphorylation
S311 Phosphorylation
S361 Phosphorylation
K399 Sumoylation
S424 Phosphorylation
K428 Sumoylation
S459 Phosphorylation
K464 Acetylation
K494 Sumoylation
T532 Phosphorylation
S543 Phosphorylation
K680 Ubiquitination
S703 Phosphorylation
T735 Phosphorylation
S737 Phosphorylation
T761 Phosphorylation
S843 Phosphorylation
K953 Sumoylation
K970 Ubiquitination
K977 Ubiquitination

Research Backgrounds

Function:

Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.

PTMs:

Phosphorylated.

Subcellular Location:

Cytoplasm. Nucleus. Endoplasmic reticulum membrane>Peripheral membrane protein.
Note: Cytoplasmic and nuclear in the absence of ligand; nuclear after ligand-binding. When bound to HSD11B2, it is found associated with the endoplasmic reticulum membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Highly expressed in distal tubules, convoluted tubules and cortical collecting duct in kidney, and in sweat glands. Detected at lower levels in cardiomyocytes, in epidermis and in colon enterocytes.

Subunit Structure:

Heteromultimeric cytoplasmic complex with HSP90, HSP70, and FKBP4, in the absence of ligand. After ligand binding, it translocates to the nucleus and binds to DNA as a homodimer and as a heterodimer with NR3C1. May interact with HSD11B2 in the absence of ligand. Binds the coactivators NCOA1, NCOA2, TIF1 and NRIP1.

Family&Domains:

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

Belongs to the nuclear hormone receptor family. NR3 subfamily.

Research Fields

· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.

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