Product: Bestrophin 2 Antibody
Catalog: DF13299
Description: Rabbit polyclonal antibody to Bestrophin 2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Rabbit
Mol.Wt.: 57kDa; 57kD(Calculated).
Uniprot: Q8NFU1
RRID: AB_2846318

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(88%)
Clonality:
Polyclonal
Specificity:
Bestrophin 2 Antibody detects endogenous levels of total Bestrophin 2.
RRID:
AB_2846318
Cite Format: Affinity Biosciences Cat# DF13299, RRID:AB_2846318.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BEST 2; Best disease Bestrophin; BEST2; Bestrophin2; FLJ20132; Vitelliform macular dystrophy 2 homolog; Vitelliform macular dystrophy 2 like 1; Vitelliform macular dystrophy 2 like protein 1; Vitelliform macular dystrophy; VMD2; VMD2 like gene 1; VMD2L1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q8NFU1 BEST2_HUMAN:

Mainly confined to the retinal pigment epithelium and colon.

Sequence:
MTVTYTARVANARFGGFSQLLLLWRGSIYKLLWRELLCFLGFYMALSAAYRFVLTEGQKRYFEKLVIYCDQYASLIPVSFVLGFYVTLVVNRWWSQYLCMPLPDALMCVVAGTVHGRDDRGRLYRRTLMRYAGLSAVLILRSVSTAVFKRFPTIDHVVEAGFMTREERKKFENLNSSYNKYWVPCVWFSNLAAQARREGRIRDNSALKLLLEELNVFRGKCGMLFHYDWISVPLVYTQVVTIALYSYFLACLIGRQFLDPAQGYKDHDLDLCVPIFTLLQFFFYAGWLKVAEQLINPFGEDDDDFETNFLIDRNFQVSMLAVDEMYDDLAVLEKDLYWDAAEARAPYTAATVFQLRQPSFQGSTFDITLAKEDMQFQRLDGLDGPMGEAPGDFLQRLLPAGAGMVAGGPLGRRLSFLLRKNSCVSEASTGASCSCAVVPEGAAPECSCGDPLLDPGLPEPEAPPPAGPEPLTLIPGPVEPFSIVTMPGPRGPAPPWLPSPIGEEEENLA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
88
Sheep
75
Bovine
63
Xenopus
50
Pig
0
Horse
0
Dog
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NFU1 As Substrate

Site PTM Type Enzyme
T113 Phosphorylation
T153 Phosphorylation
T164 Phosphorylation
S415 Phosphorylation

Research Backgrounds

Function:

Forms calcium-sensitive chloride channels. Permeable to bicarbonate.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Mainly confined to the retinal pigment epithelium and colon.

Family&Domains:

Belongs to the bestrophin family.

Research Fields

· Organismal Systems > Digestive system > Salivary secretion.

Restrictive clause

 

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For Research Use Only.
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