SCXA Antibody | Affinity Biosciences

Product:	SCXA Antibody
Catalog:	DF13293
Description:	Rabbit polyclonal antibody to SCXA
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse
Mol.Wt.:	21kDa; 22kD(Calculated).
Uniprot:	Q7RTU7
RRID:	AB_2846312

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%)

Clonality:

Polyclonal

Specificity:

SCXA Antibody detects endogenous levels of total SCXA.

RRID:

AB_2846312
Cite Format: Affinity Biosciences Cat# DF13293, RRID:AB_2846312.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Basic helix loop helix transcription factor scleraxis; Basic helix-loop-helix transcription factor scleraxis; bHLHa41; bHLHa48; Class A basic helix-loop-helix protein 41; Class A basic helix-loop-helix protein 48; scleraxis; scleraxis homolog A; SCX; SCX_HUMAN; SCXB;

Immunogens

Immunogen:

Uniprot:

Q7RTU7(SCX_HUMAN) >>Visit HPA database.

Gene(ID):

SCX(642658)

Sequence:

Q7RTU7 SCX_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSFATLRPAPPGRYLYPEVSPLSEDEDRGSDSSGSDEKPCRVHAARCGLQGARRRAGGRRAGGGGPGGRPGREPRQRHTANARERDRTNSVNTAFTALRTLIPTEPADRKLSKIETLRLASSYISHLGNVLLAGEACGDGQPCHSGPAFFHAARAGSPPPPPPPPPARDGENTQPKQICTFCLSNQRKLSKDRDRKTAIRS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7RTU7 As Substrate

Q7RTU7

Site	PTM Type	Source
T5	Phosphorylation	Uniprot
S30	Phosphorylation	Uniprot
S32	Phosphorylation	Uniprot
S33	Phosphorylation	Uniprot
S35	Phosphorylation	Uniprot
T100	Phosphorylation	Uniprot
S157	Phosphorylation	Uniprot

Research Backgrounds

Q7RTU7_SCX_HUMAN

Function:

Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.

Subcellular Location:

Nucleus.

Subunit Structure:

Efficient DNA binding requires dimerization with another bHLH protein. Dimerizes and binds the E-box consensus sequence with E12 (By similarity).

References

1). Harnessing Mechanical Stress with Viscoelastic Biomaterials for Periodontal Ligament Regeneration. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024 (PubMed: 38460171) [IF=15.1]

2). Small extracellular vesicles derived from tendon stem cells promote the healing of injured Achilles tendons by regulating miR-145-3p. Acta biomaterialia, 2023 (PubMed: 37806377) [IF=9.7]

3). Adipose-derived mesenchymal stromal cell-derived exosomes promote tendon healing by activating both SMAD1/5/9 and SMAD2/3. Stem Cell Research & Therapy, 2021 (PubMed: 34112236) [IF=7.5]

Application: IHC Species: rat Sample: tendon

Fig. 5 |ADSC-Exos improved the healing of tendon injury. a, h The H&E staining of tendon injury at week 2 and week 4. b–f, i–m The expression of TNMD, collagen I, SCXA, ALP, and Runx2 were detected by immunohistochemistry assay at week 2 (n = 6) and week 4 (n = 6).

4). Eugenol-Preconditioned Mesenchymal Stem Cell-Derived Extracellular Vesicles Promote Antioxidant Capacity of Tendon Stem Cells In Vitro and In Vivo. Oxidative Medicine and Cellular Longevity, 2022 (PubMed: 35178155)

Application: WB Species: Rat Sample: TSCs

Figure 3 EUG-BMSC-EVs protected tenogenic differentiation of H2O2-treated TSCs. (a–e) Western blot was used to examine the expression of SCXA, TNMD, TNC, and COLI. (f–i) The expression of tenocyte-related genes, SCX, TNMD, TNC, and Col1a1. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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SCXA Antibody - #DF13293