Rab4 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/6

Western blot analysis of extracts from A2780 cells, using Rab4 Antibody.

DF13290 at 1/100 staining Human colorectal cancer by IHC-P.

DF13290 at 1/100 staining Human pancreatic cancer and adjacent nomal tissues by IHC-P.

DF13290 at 1/100 staining Mouse liver tissue by IHC-P.

DF13290 at 1/100 staining Rat liver tissue by IHC-P.

DF13290 staining Hela cells by IF/ICC.

Product:	Rab4 Antibody
Catalog:	DF13290
Description:	Rabbit polyclonal antibody to Rab4
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	24kDa; 24kD(Calculated).
Uniprot:	P20338
RRID:	AB_2846309

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

Rab4 Antibody detects endogenous levels of total Rab4.

RRID:

AB_2846309
Cite Format: Affinity Biosciences Cat# DF13290, RRID:AB_2846309.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

HRES 1 / RAB4; Oncogene RAB4; Rab 4; RAB 4A; RAB4 member RAS oncogene family; Rab4a; RAB4A member RAS oncogene family; RAB4A_HUMAN; Ras related protein Rab 4A; Ras related protein Rab4A; Ras-related protein Rab-4A;

Immunogens

Immunogen:

Uniprot:

P20338(RAB4A_HUMAN) >>Visit HPA database.

Gene(ID):

RAB4A(5867)

Sequence:

P20338 RAB4A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSQTAMSETYDFLFKFLVIGNAGTGKSCLLHQFIEKKFKDDSNHTIGVEFGSKIINVGGKYVKLQIWDTAGQERFRSVTRSYYRGAAGALLVYDITSRETYNALTNWLTDARMLASQNIVIILCGNKKDLDADREVTFLEASRFAQENELMFLETSALTGENVEEAFVQCARKILNKIESGELDPERMGSGIQYGDAALRQLRSPRRAQAPNAQECGC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Zebrafish

100

Chicken

100

Rabbit

100

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P20338 As Substrate

P20338

Site	PTM Type	Enzyme	Source
T24	Phosphorylation		Uniprot
K63	Ubiquitination		Uniprot
T69	Phosphorylation		Uniprot
T137	Phosphorylation		Uniprot
S142	Phosphorylation		Uniprot
S190	Phosphorylation		Uniprot
Y194	Phosphorylation		Uniprot
S204	Phosphorylation	P06493 (CDK1)	Uniprot

Research Backgrounds

P20338_RAB4A_HUMAN

Function:

Protein transport. Plays a role in vesicular traffic. Mediates VEGFR2 endosomal trafficking to enhance VEGFR2 signaling.

PTMs:

Phosphorylated by CDK1 kinase during mitosis.

Subcellular Location:

Membrane>Peripheral membrane protein. Cytoplasm. Early endosome membrane>Peripheral membrane protein. Recycling endosome membrane>Peripheral membrane protein.
Note: Generally associated with membranes. Cytoplasmic when phosphorylated by CDK1.

Subunit Structure:

Interacts with SGSM1, SGSM2 and SGSM3 (By similarity). Interacts with RAB11FIP1, RABEP1, ZFYVE20 and RUFY1. Interacts (membrane-bound form) with NDRG1; the interaction involves NDRG1 in vesicular recycling of E-cadherin. Interacts (in GTP-bound form) with GRIPAP1 (via N-terminus). Interacts with RABEP1 and RBSN. Does not interact with HPS4 (By similarity). Interacts with RABEP2; this interaction may mediate VEGFR2 cell surface expression.

Family&Domains:

Belongs to the small GTPase superfamily. Rab family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

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Rab4 Antibody - #DF13290