Myelin PLP Antibody - #DF13282

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Product: Myelin PLP Antibody
Catalog: DF13282
Description: Rabbit polyclonal antibody to Myelin PLP
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 30kDa; 30kD(Calculated).
Uniprot: P60201
RRID: AB_2846301

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Myelin PLP Antibody detects endogenous levels of total Myelin PLP.
RRID:
AB_2846301
Cite Format: Affinity Biosciences Cat# DF13282, RRID:AB_2846301.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HLD1; Lipophilin; Major myelin proteolipid protein; MMPL; Myelin proteolipid protein; MYPR_HUMAN; PLP 1; PLP; PLP/DM20; PLP1; PMD; Proteolipid protein 1; SPG2;

Immunogens

Immunogen:
Uniprot:

P60201(MYPR_HUMAN) >>Visit HPA database.

Gene(ID):
PLP1(5354)
Sequence:
MGLLECCARCLVGAPFASLVATGLCFFGVALFCGCGHEALTGTEKLIETYFSKNYQDYEYLINVIHAFQYVIYGTASFFFLYGALLLAEGFYTTGAVRQIFGDYKTTICGKGLSATVTGGQKGRGSRGQHQAHSLERVCHCLGKWLGHPDKFVGITYALTVVWLLVFACSAVPVYIYFNTWTTCQSIAFPSKTSASIGSLCADARMYGVLPWNAFPGKVCGSNLLSICKTAEFQMTFHLFIAAFVGAAATLVSLLTFMIAATYNFAVLKLMGRGTKF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
50
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P60201 As Substrate

Site PTM Type Enzyme
S114 Phosphorylation
T116 Phosphorylation
T118 Phosphorylation

Research Backgrounds

Function:

This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Myelin membrane.
Note: Colocalizes with SIRT2 in internodal regions, at paranodal axoglial junction and Schmidt-Lanterman incisures of myelin sheat.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the myelin proteolipid protein family.

References

1). Proteomic analysis of the effects of caffeine in a neonatal rat model of hypoxic‐ischemic white matter damage. CNS Neuroscience & Therapeutics, 2022 (PubMed: 35393758) [IF=5.5]

Application: WB    Species: Rat    Sample: brain tissue

FIGURE 3 Caffeine improved myelin development after hypoxic‐ischemic WMD in neonatal rats. (A) Western blot detection of MBP, PLP, and MAG. Relative protein levels of (B) MBP, (C) PLP, and (D) MAG. (E) Immunohistochemical staining of MBP and LFB staining in the corpus callosum (CC) area of the ligated side cerebral hemisphere at 14 days after hypoxic‐ischemia (MBP scale bar = 50 μm; LBF scale bar = 50 μm). Average optical density (AOD) values of (F) MBP and (G) LBF staining. (H) Diagrammatic representation of the brain region analyzed for the level of MBP and LBF staining. Data are presented as means ± SEMs. Statistical analyses involved one‐way ANOVA, followed by Tukey's test. *p < 0.05, **p < 0.01, ***p < 0.001. Sham group (n = 6); HI group (n = 6); Caffeine group (n = 6); and Caffeine+AK‐7 group (n = 6). Ca, Caffeine group; Ca+AK‐7, Caffeine+AK‐7 group
2). Identification and preliminary analysis of hub genes associated with bladder cancer progression by comprehensive bioinformatics analysis. Scientific reports, 2024 (PubMed: 38307969) [IF=4.6]

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