FCER1G Antibody - #DF13263

(1)   (4)  
Product: FCER1G Antibody
Catalog: DF13263
Description: Rabbit polyclonal antibody to FCER1G
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 9kDa; 10kD(Calculated).
Uniprot: P30273
RRID: AB_2846282

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
FCER1G Antibody detects endogenous levels of total FCER1G.
RRID:
AB_2846282
Cite Format: Affinity Biosciences Cat# DF13263, RRID:AB_2846282.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide; Fc receptor gamma chain; Fc-epsilon RI-gamma; FCER1G; FCERG_HUMAN; FceRI gamma; FCRG; FcRgamma; High affinity immunoglobulin epsilon receptor subunit gamma; IgE Fc receptor subunit gamma; Immunoglobulin E receptor, high affinity, gamma chain;

Immunogens

Immunogen:
Uniprot:

P30273(FCERG_HUMAN) >>Visit HPA database.

Gene(ID):
FCER1G(2207)
Sequence:
MIPAVVLLLLLLVEQAAALGEPQLCYILDAILFLYGIVLTLLYCRLKIQVRKAAITSYEKSDGVYTGLSTRNQETYETLKHEKPPQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30273 As Substrate

Site PTM Type Enzyme
Y58 Phosphorylation
K60 Ubiquitination
S61 Phosphorylation
Y65 Phosphorylation
T66 Phosphorylation
S69 Phosphorylation
T70 Phosphorylation
T75 Phosphorylation
Y76 Phosphorylation
T78 Phosphorylation

Research Backgrounds

Function:

Adapter protein containing an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors. As a component of the high-affinity immunoglobulin E (IgE) receptor, mediates allergic inflammatory signaling in mast cells. As a constitutive component of interleukin-3 receptor complex, selectively mediates interleukin 4/IL4 production by basophils, priming T-cells toward effector T-helper 2 subset. Associates with pattern recognition receptors CLEC4D and CLEC4E to form a functional signaling complex in myeloid cells. Binding of mycobacterial trehalose 6,6'-dimycolate (TDM) to this receptor complex leads to phosphorylation of ITAM, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes. May function cooperatively with other activating receptors. Functionally linked to integrin beta-2/ITGB2-mediated neutrophil activation. Also involved in integrin alpha-2/ITGA2-mediated platelet activation.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

IgE Fc receptor is a tetramer of an alpha chain, a beta chain, and two disulfide-linked gamma chains. Associates with FCGR1A; forms a functional signaling complex. Associated with CLEC6A (By similarity). Interacts with CLEC4E. Interacts (via ITAM domain) with SYK (via SH2 domains); activates SYK, enabling integrin-mediated activation of neutrophils and macrophages (By similarity). Interacts with CSF2RB and recruits SYK in response to IL3 stimulation; this interaction is direct (By similarity). Interacts with CD300LH; the interaction may be indirect (By similarity). Interacts with CD300LD (By similarity). Interacts with TARM1.

Family&Domains:

Belongs to the CD3Z/FCER1G family.

References

1). Comprehensive landscape of immune-based classifier related to early diagnosis and macrophage M1 in spinal cord injury. Aging (Albany NY) (PubMed: 36842142) [IF=5.2]

Application: WB    Species: Rat    Sample:

Figure 10 Verification of the expression of Core IRGs via rat SCI model. (A, B) Sequencing results of Core IRGs in SCI and sham group; (C) WB analysis demonstrating the expression of Core IRGs in the sham, SCI-3d and 7d group, respectively; (D, E) Immunofluorescence showed the expression of Core IRGs in sham and SCI-7d groups. Bar = 50 um; *p < 0.05; **p < 0.01; and ***p < 0.001.

Application: IF/ICC    Species: Rat    Sample:

Figure 10 Verification of the expression of Core IRGs via rat SCI model. (A, B) Sequencing results of Core IRGs in SCI and sham group; (C) WB analysis demonstrating the expression of Core IRGs in the sham, SCI-3d and 7d group, respectively; (D, E) Immunofluorescence showed the expression of Core IRGs in sham and SCI-7d groups. Bar = 50 um; *p < 0.05; **p < 0.01; and ***p < 0.001.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.