Mannosidase II Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/5

Western blot analysis of extracts from COS7, using Mannosidase II Antibody.

DF13260 at 1/100 staining Human colorectal cancer by IHC-P.

DF13260 at 1/100 staining Mouse testis tissue by IHC-P.

DF13260 at 1/100 staining Rat lung tissue by IHC-P.

DF13260 staining HepG2 cells by IF/ICC.

Product:	Mannosidase II Antibody
Catalog:	DF13260
Description:	Rabbit polyclonal antibody to Mannosidase II
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat, Monkey
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	131kDa; 131kD(Calculated).
Uniprot:	Q16706
RRID:	AB_2846279

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Prediction:

Pig(88%), Bovine(88%), Sheep(88%), Rabbit(100%), Dog(88%), Chicken(88%), Xenopus(88%)

Clonality:

Polyclonal

Specificity:

Mannosidase II Antibody detects endogenous levels of total Mannosidase II.

RRID:

AB_2846279
Cite Format: Affinity Biosciences Cat# DF13260, RRID:AB_2846279.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

3-1; 6-alpha-mannosidase; Alpha mannosidase 2; alpha Mannosidase II; Alpha-mannosidase 2; AMAN II; Golgi alpha mannosidase II; Golgi alpha-mannosidase II; Golgi integral membrane protein 7; GOLIM7; MA2A1_HUMAN; MAN II; Man2a1; MANA 2; MANA2; MANII; Mann II; Mannosidase alpha class 2A member 1; Mannosidase Two; Mannosidase, alpha type II; Mannosidase, alpha, II; Mannosyl oligosaccharide 1 3 1 6 alpha mannosidase; Mannosyl oligosaccharide 1,3 1,6 alpha mannosidase; Mannosyl-oligosaccharide 1;

Immunogens

Immunogen:

Uniprot:

Q16706(MA2A1_HUMAN) >>Visit HPA database.

Gene(ID):

MAN2A1(4124)

Sequence:

Q16706 MA2A1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKLSRQFTVFGSAIFCVVIFSLYLMLDRGHLDYPRNPRREGSFPQGQLSMLQEKIDHLERLLAENNEIISNIRDSVINLSESVEDGPKSSQSNFSQGAGSHLLPSQLSLSVDTADCLFASQSGSHNSDVQMLDVYSLISFDNPDGGVWKQGFDITYESNEWDTEPLQVFVVPHSHNDPGWLKTFNDYFRDKTQYIFNNMVLKLKEDSRRKFIWSEISYLSKWWDIIDIQKKDAVKSLIENGQLEIVTGGWVMPDEATPHYFALIDQLIEGHQWLENNIGVKPRSGWAIDPFGHSPTMAYLLNRAGLSHMLIQRVHYAVKKHFALHKTLEFFWRQNWDLGSVTDILCHMMPFYSYDIPHTCGPDPKICCQFDFKRLPGGRFGCPWGVPPETIHPGNVQSRARMLLDQYRKKSKLFRTKVLLAPLGDDFRYCEYTEWDLQFKNYQQLFDYMNSQSKFKVKIQFGTLSDFFDALDKADETQRDKGQSMFPVLSGDFFTYADRDDHYWSGYFTSRPFYKRMDRIMESHLRAAEILYYFALRQAHKYKINKFLSSSLYTALTEARRNLGLFQHHDAITGTAKDWVVVDYGTRLFHSLMVLEKIIGNSAFLLILKDKLTYDSYSPDTFLEMDLKQKSQDSLPQKNIIRLSAEPRYLVVYNPLEQDRISLVSVYVSSPTVQVFSASGKPVEVQVSAVWDTANTISETAYEISFRAHIPPLGLKVYKILESASSNSHLADYVLYKNKVEDSGIFTIKNMINTEEGITLENSFVLLRFDQTGLMKQMMTKEDGKHHEVNVQFSWYGTTIKRDKSGAYLFLPDGNAKPYVYTTPPFVRVTHGRIYSEVTCFFDHVTHRVRLYHIQGIEGQSVEVSNIVDIRKVYNREIAMKISSDIKSQNRFYTDLNGYQIQPRMTLSKLPLQANVYPMTTMAYIQDAKHRLTLLSAQSLGVSSLNSGQIEVIMDRRLMQDDNRGLEQGIQDNKITANLFRILLEKRSAVNTEEEKKSVSYPSLLSHITSSLMNHPVIPMANKFSSPTLELQGEFSPLQSSLPCDIHLVNLRTIQSKVGNGHSNEAALILHRKGFDCRFSSKGTGLFCSTTQGKILVQKLLNKFIVESLTPSSLSLMHSPPGTQNISEINLSPMEISTFRIQLR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Bovine

Sheep

Dog

Xenopus

Chicken

Zebrafish

Horse

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16706 As Substrate

Q16706

Site	PTM Type	Source
Y33	Phosphorylation	Uniprot
S75	Phosphorylation	Uniprot
N78	N-Glycosylation	Uniprot
S80	Phosphorylation	Uniprot
S82	Phosphorylation	Uniprot
S105	O-Glycosylation	Uniprot
T192	Phosphorylation	Uniprot
Y194	Phosphorylation	Uniprot
Y218	Phosphorylation	Uniprot
S307	Phosphorylation	Uniprot
K373	Ubiquitination	Uniprot
Y407	Phosphorylation	Uniprot
Y442	Phosphorylation	Uniprot
K473	Ubiquitination	Uniprot
T509	Phosphorylation	Uniprot
Y514	Phosphorylation	Uniprot
Y532	Phosphorylation	Uniprot
Y533	Phosphorylation	Uniprot
K716	Ubiquitination	Uniprot
S723	Phosphorylation	Uniprot
Y733	Phosphorylation	Uniprot
K737	Ubiquitination	Uniprot
K739	Ubiquitination	Uniprot
S743	Phosphorylation	Uniprot
T747	Phosphorylation	Uniprot
K801	Ubiquitination	Uniprot
Y852	Phosphorylation	Uniprot
S865	Phosphorylation	Uniprot
K887	Ubiquitination	Uniprot
S998	Phosphorylation	Uniprot
N1125	N-Glycosylation	Uniprot

Research Backgrounds

Q16706_MA2A1_HUMAN

Function:

Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.

PTMs:

Glycosylated.

Subcellular Location:

Golgi apparatus membrane>Single-pass type II membrane protein.

Subunit Structure:

Homodimer; disulfide-linked.

Family&Domains:

Belongs to the glycosyl hydrolase 38 family.

Research Fields

· Metabolism > Glycan biosynthesis and metabolism > N-Glycan biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). PA-MSHA Regulates PD-L1 Expression in Hepatoma Cells. Immunological Investigations (PubMed: 36762677) [IF=2.8]

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Mannosidase II Antibody - #DF13260