Mannosidase II Antibody - #DF13260

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Product: Mannosidase II Antibody
Catalog: DF13260
Description: Rabbit polyclonal antibody to Mannosidase II
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 131kDa; 131kD(Calculated).
Uniprot: Q16706
RRID: AB_2846279

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(88%), Bovine(88%), Sheep(88%), Rabbit(100%), Dog(88%), Chicken(88%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
Mannosidase II Antibody detects endogenous levels of total Mannosidase II.
RRID:
AB_2846279
Cite Format: Affinity Biosciences Cat# DF13260, RRID:AB_2846279.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3-1; 6-alpha-mannosidase; Alpha mannosidase 2; alpha Mannosidase II; Alpha-mannosidase 2; AMAN II; Golgi alpha mannosidase II; Golgi alpha-mannosidase II; Golgi integral membrane protein 7; GOLIM7; MA2A1_HUMAN; MAN II; Man2a1; MANA 2; MANA2; MANII; Mann II; Mannosidase alpha class 2A member 1; Mannosidase Two; Mannosidase, alpha type II; Mannosidase, alpha, II; Mannosyl oligosaccharide 1 3 1 6 alpha mannosidase; Mannosyl oligosaccharide 1,3 1,6 alpha mannosidase; Mannosyl-oligosaccharide 1;

Immunogens

Immunogen:
Uniprot:

Q16706(MA2A1_HUMAN) >>Visit HPA database.

Gene(ID):
MAN2A1(4124)
Sequence:
MKLSRQFTVFGSAIFCVVIFSLYLMLDRGHLDYPRNPRREGSFPQGQLSMLQEKIDHLERLLAENNEIISNIRDSVINLSESVEDGPKSSQSNFSQGAGSHLLPSQLSLSVDTADCLFASQSGSHNSDVQMLDVYSLISFDNPDGGVWKQGFDITYESNEWDTEPLQVFVVPHSHNDPGWLKTFNDYFRDKTQYIFNNMVLKLKEDSRRKFIWSEISYLSKWWDIIDIQKKDAVKSLIENGQLEIVTGGWVMPDEATPHYFALIDQLIEGHQWLENNIGVKPRSGWAIDPFGHSPTMAYLLNRAGLSHMLIQRVHYAVKKHFALHKTLEFFWRQNWDLGSVTDILCHMMPFYSYDIPHTCGPDPKICCQFDFKRLPGGRFGCPWGVPPETIHPGNVQSRARMLLDQYRKKSKLFRTKVLLAPLGDDFRYCEYTEWDLQFKNYQQLFDYMNSQSKFKVKIQFGTLSDFFDALDKADETQRDKGQSMFPVLSGDFFTYADRDDHYWSGYFTSRPFYKRMDRIMESHLRAAEILYYFALRQAHKYKINKFLSSSLYTALTEARRNLGLFQHHDAITGTAKDWVVVDYGTRLFHSLMVLEKIIGNSAFLLILKDKLTYDSYSPDTFLEMDLKQKSQDSLPQKNIIRLSAEPRYLVVYNPLEQDRISLVSVYVSSPTVQVFSASGKPVEVQVSAVWDTANTISETAYEISFRAHIPPLGLKVYKILESASSNSHLADYVLYKNKVEDSGIFTIKNMINTEEGITLENSFVLLRFDQTGLMKQMMTKEDGKHHEVNVQFSWYGTTIKRDKSGAYLFLPDGNAKPYVYTTPPFVRVTHGRIYSEVTCFFDHVTHRVRLYHIQGIEGQSVEVSNIVDIRKVYNREIAMKISSDIKSQNRFYTDLNGYQIQPRMTLSKLPLQANVYPMTTMAYIQDAKHRLTLLSAQSLGVSSLNSGQIEVIMDRRLMQDDNRGLEQGIQDNKITANLFRILLEKRSAVNTEEEKKSVSYPSLLSHITSSLMNHPVIPMANKFSSPTLELQGEFSPLQSSLPCDIHLVNLRTIQSKVGNGHSNEAALILHRKGFDCRFSSKGTGLFCSTTQGKILVQKLLNKFIVESLTPSSLSLMHSPPGTQNISEINLSPMEISTFRIQLR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
88
Bovine
88
Sheep
88
Dog
88
Xenopus
88
Chicken
88
Zebrafish
75
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16706 As Substrate

Site PTM Type Enzyme
Y33 Phosphorylation
S75 Phosphorylation
N78 N-Glycosylation
S80 Phosphorylation
S82 Phosphorylation
S105 O-Glycosylation
T192 Phosphorylation
Y194 Phosphorylation
Y218 Phosphorylation
S307 Phosphorylation
K373 Ubiquitination
Y407 Phosphorylation
Y442 Phosphorylation
K473 Ubiquitination
T509 Phosphorylation
Y514 Phosphorylation
Y532 Phosphorylation
Y533 Phosphorylation
K716 Ubiquitination
S723 Phosphorylation
Y733 Phosphorylation
K737 Ubiquitination
K739 Ubiquitination
S743 Phosphorylation
T747 Phosphorylation
K801 Ubiquitination
Y852 Phosphorylation
S865 Phosphorylation
K887 Ubiquitination
S998 Phosphorylation
N1125 N-Glycosylation

Research Backgrounds

Function:

Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.

PTMs:

Glycosylated.

Subcellular Location:

Golgi apparatus membrane>Single-pass type II membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer; disulfide-linked.

Family&Domains:

Belongs to the glycosyl hydrolase 38 family.

Research Fields

· Metabolism > Glycan biosynthesis and metabolism > N-Glycan biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). PA-MSHA Regulates PD-L1 Expression in Hepatoma Cells. Immunological Investigations, 2023 (PubMed: 36762677) [IF=2.8]

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