cbx7 Antibody - #DF13257

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Product: cbx7 Antibody
Catalog: DF13257
Description: Rabbit polyclonal antibody to cbx7
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 28kDa; 28kD(Calculated).
Uniprot: O95931
RRID: AB_2846276

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
cbx7 Antibody detects endogenous levels of total cbx7.
RRID:
AB_2846276
Cite Format: Affinity Biosciences Cat# DF13257, RRID:AB_2846276.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cbx 7; CBX7; Chromobox homolog 7; Chromobox protein homolog 7;

Immunogens

Immunogen:
Uniprot:

O95931(CBX7_HUMAN) >>Visit HPA database.

Gene(ID):
CBX7(23492)
Sequence:
MELSAIGEQVFAVESIRKKRVRKGKVEYLVKWKGWPPKYSTWEPEEHILDPRLVMAYEEKEERDRASGYRKRGPKPKRLLLQRLYSMDLRSSHKAKGKEKLCFSLTCPLGSGSPEGVVKAGAPELVDKGPLVPTLPFPLRKPRKAHKYLRLSRKKFPPRGPNLESHSHRRELFLQEPPAPDVLQAAGEWEPAAQPPEEEADADLAEGPPPWTPALPSSEVTVTDITANSITVTFREAQAAEGFFRDRSGKF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
88
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95931 As Substrate

Site PTM Type Enzyme
S15 Phosphorylation
K75 Acetylation
K77 Acetylation
Y85 Phosphorylation
S86 Phosphorylation
S91 Phosphorylation
S92 Phosphorylation
T134 Phosphorylation
S167 Phosphorylation

Research Backgrounds

Function:

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Promotes histone H3 trimethylation at 'Lys-9' (H3K9me3). Binds to trimethylated lysine residues in histones, and possibly also other proteins. Regulator of cellular lifespan by maintaining the repression of CDKN2A, but not by inducing telomerase activity.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of a PRC1-like complex. Interacts with RING1 and RNF2/RING1B, but not with BMI1, EED or EZH2. Interacts with PCGF1, PCGF2, PCGF3, PCGF5 and PCGF6.

References

1). Identification of the Prognostic Biomarkers CBX6 and CBX7 in Bladder Cancer. Diagnostics (Basel, Switzerland), 2023 (PubMed: 37189494) [IF=3.6]

Application: WB    Species: Human    Sample: BLCA cell and tissue

Figure 11. Validation of CBX family protein expression levels in BLCA cell and tissue samples. (A) The expression levels of CBX family proteins were detected by Western blotting in normal urothelial cell lines (SV-HUC-1) and BLCA cell lines (5637 and T24) (n = 3). (B–I) Quantitative results of (A). (J,K) Expression levels of CBX6, CD25, and CD86 in BLCA tumor tissue samples analyzed by immunohistochemistry (IHC). (L,M) Expression level of CBX7, KIT, and CD14 analyzed by IHC in BLCA tumor tissue samples. * p < 0.05; ** p < 0.01; *** p < 0.001.

Application: IF/ICC    Species: Human    Sample: BLCA cell and tissue

Figure 11. Validation of CBX family protein expression levels in BLCA cell and tissue samples. (A) The expression levels of CBX family proteins were detected by Western blotting in normal urothelial cell lines (SV-HUC-1) and BLCA cell lines (5637 and T24) (n = 3). (B–I) Quantitative results of (A). (J,K) Expression levels of CBX6, CD25, and CD86 in BLCA tumor tissue samples analyzed by immunohistochemistry (IHC). (L,M) Expression level of CBX7, KIT, and CD14 analyzed by IHC in BLCA tumor tissue samples. * p < 0.05; ** p < 0.01; *** p < 0.001.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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