TRPC4 Antibody - #DF13254
| Product: | TRPC4 Antibody |
| Catalog: | DF13254 |
| Description: | Rabbit polyclonal antibody to TRPC4 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Mol.Wt.: | 112kDa,90kDa; 112kD(Calculated). |
| Uniprot: | Q9UBN4 |
| RRID: | AB_2846273 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13254, RRID:AB_2846273.
Fold/Unfold
CCE1; HTRP 4; hTrp-4; hTrp4; MGC119570; MGC119571; MGC119572; MGC119573; RP11-10M8.1; Short transient receptor potential channel 4; STRPC4; Transient receptor potential 4; transient receptor potential cation channel subfamily C; Transient receptor potential cation channel subfamily C member 4; transient receptor potential channel 4; TRP 4; trp related protein 4; Trp-related protein 4; TRP4; TRPC 4; TrpC4; TRPC4 protein; TRPC4_HUMAN;
Immunogens
Strongly expressed in placenta. Expressed at lower levels in heart, pancreas, kidney and brain. Expressed in endothelial cells. Isoform alpha was found to be the predominant isoform. Isoform beta was not found in pancreas and brain.
- Q9UBN4 TRPC4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQFYYKRNVNAPYRDRIPLRIVRAESELSPSEKAYLNAVEKGDYASVKKSLEEAEIYFKININCIDPLGRTALLIAIENENLELIELLLSFNVYVGDALLHAIRKEVVGAVELLLNHKKPSGEKQVPPILLDKQFSEFTPDITPIILAAHTNNYEIIKLLVQKGVSVPRPHEVRCNCVECVSSSDVDSLRHSRSRLNIYKALASPSLIALSSEDPFLTAFQLSWELQELSKVENEFKSEYEELSRQCKQFAKDLLDQTRSSRELEIILNYRDDNSLIEEQSGNDLARLKLAIKYRQKEFVAQPNCQQLLASRWYDEFPGWRRRHWAVKMVTCFIIGLLFPVFSVCYLIAPKSPLGLFIRKPFIKFICHTASYLTFLFLLLLASQHIDRSDLNRQGPPPTIVEWMILPWVLGFIWGEIKQMWDGGLQDYIHDWWNLMDFVMNSLYLATISLKIVAFVKYSALNPRESWDMWHPTLVAEALFAIANIFSSLRLISLFTANSHLGPLQISLGRMLLDILKFLFIYCLVLLAFANGLNQLYFYYEETKGLTCKGIRCEKQNNAFSTLFETLQSLFWSIFGLINLYVTNVKAQHEFTEFVGATMFGTYNVISLVVLLNMLIAMMNNSYQLIADHADIEWKFARTKLWMSYFEEGGTLPTPFNVIPSPKSLWYLIKWIWTHLCKKKMRRKPESFGTIGRRAADNLRRHHQYQEVMRNLVKRYVAAMIRDAKTEEGLTEENFKELKQDISSFRFEVLGLLRGSKLSTIQSANASKESSNSADSDEKSDSEGNSKDKKKNFSLFDLTTLIHPRSAAIASERHNISNGSALVVQEPPREKQRKVNFVTDIKNFGLFHRRSKQNAAEQNANQIFSVSEEVARQQAAGPLERNIQLESRGLASRGDLSIPGLSEQCVLVDHRERNTDTLGLQVGKRVCPFKSEKVVVEDTVPIIPKEKHAKEEDSSIDYDLNLPDTVTHEDYVTTRL
PTMs - Q9UBN4 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S205 | Phosphorylation | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| Y624 | Phosphorylation | Uniprot | |
| S688 | Phosphorylation | Uniprot | |
| S781 | Phosphorylation | Uniprot | |
| S783 | Phosphorylation | Uniprot | |
| S787 | Phosphorylation | Uniprot | |
| S955 | Phosphorylation | Uniprot | |
| S956 | Phosphorylation | Uniprot | |
| Y959 | Phosphorylation | Uniprot | |
| Y972 | Phosphorylation | Uniprot |
Research Backgrounds
Forms a receptor-activated non-selective calcium permeant cation channel. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Mediates cation entry, with an enhanced permeability to barium over calcium. May also be activated by intracellular calcium store depletion.
Phosphorylation modulates TRPC channel function by regulating the level of TRPC4 at the cell surface and by increasing the association with SLC9A3R1/NHERF.
Membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.
Note: Enhanced insertion into the cell membrane after activation of the EGF receptor.
Strongly expressed in placenta. Expressed at lower levels in heart, pancreas, kidney and brain. Expressed in endothelial cells. Isoform alpha was found to be the predominant isoform. Isoform beta was not found in pancreas and brain.
Interacts with TRPC4AP (By similarity). Homotetramer and heterotetramer with TRPC1 and/or TRPC5. Isoform alpha but not isoform beta associates with inositol 1,4,5-trisphosphate receptor (ITPR). Interacts with (via PDZ-binding domain) with SLC9A3R1/NHERF. Interacts with MX1 and RNF24. Interacts (via CIRB domain) with SESTD1 (via spectrin 1 repeat). Interacts with CDH5 and CTNNB1. Interacts with SPTAN1 (via C-terminal spectrin repeats) and SPTBN5 (via C-terminus). Interacts (via protein 4.1-binding domain) with EPB41L2.
The protein 4.1-binding domain (654-685) is required for binding to EPB41L2 and channel activation.
The calmodulin- and inositol 1,4,5-trisphosphate receptor-binding (CIRB) domain (695-724) is sufficient for the interaction with SESTD1.
The spectrin-binding domain (730-758) is required for binding to SPTAN1 and SPTBN5.
Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.
Research Fields
· Organismal Systems > Development > Axon guidance. (View pathway)
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