SCD1 Antibody - #DF13253

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Product: SCD1 Antibody
Catalog: DF13253
Description: Rabbit polyclonal antibody to SCD1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 41kDa; 42kD(Calculated).
Uniprot: O00767
RRID: AB_2846272

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
SCD1 Antibody detects endogenous levels of total SCD1.
RRID:
AB_2846272
Cite Format: Affinity Biosciences Cat# DF13253, RRID:AB_2846272.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACOD_HUMAN; Acyl-CoA desaturase; Delta(9)-desaturase; Delta-9 desaturase; Delta-9-Desaturase; FADS5; Fatty acid desaturase; PRO0998; SCD 1; SCD; SCD1; Stearoyl-CoA desaturase;

Immunogens

Immunogen:
Uniprot:

O00767(ACOD_HUMAN) >>Visit HPA database.

Gene(ID):
SCD(6319)
Expression:
O00767 ACOD_HUMAN:

Detected in fetal liver, lung and brain. Highly expressed in adult adipose tissue, and at lower levels in adult brain and lung.

Sequence:
MPAHLLQDDISSSYTTTTTITAPPSRVLQNGGDKLETMPLYLEDDIRPDIKDDIYDPTYKDKEGPSPKVEYVWRNIILMSLLHLGALYGITLIPTCKFYTWLWGVFYYFVSALGITAGAHRLWSHRSYKARLPLRLFLIIANTMAFQNDVYEWARDHRAHHKFSETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGSTLDLSDLEAEKLVMFQRRYYKPGLLMMCFILPTLVPWYFWGETFQNSVFVATFLRYAVVLNATWLVNSAAHLFGYRPYDKNISPRENILVSLGAVGEGFHNYHHSFPYDYSASEYRWHINFTTFFIDCMAALGLAYDRKKVSKAAILARIKRTGDGNYKSG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Horse
57
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00767 As Substrate

Site PTM Type Enzyme
Y14 Phosphorylation
K34 Ubiquitination
Y41 Phosphorylation
K51 Sumoylation
K51 Ubiquitination
Y55 Phosphorylation
Y59 Phosphorylation
K60 Ubiquitination
K62 Ubiquitination
K68 Sumoylation
K68 Ubiquitination
S80 Phosphorylation
T91 Phosphorylation
T95 Phosphorylation
K162 Ubiquitination
K189 Ubiquitination
K194 Ubiquitination
K196 Ubiquitination
S198 Phosphorylation
T199 Phosphorylation
S203 Phosphorylation
K209 Ubiquitination
K278 Ubiquitination
K338 Ubiquitination
K341 Ubiquitination
T351 Phosphorylation
K357 Ubiquitination

Research Backgrounds

Function:

Stearyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates. Catalyzes the insertion of a cis double bond at the delta-9 position into fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Gives rise to a mixture of 16:1 and 18:1 unsaturated fatty acids. Plays an important role in lipid biosynthesis. Plays an important role in regulating the expression of genes that are involved in lipogenesis and in regulating mitochondrial fatty acid oxidation (By similarity). Plays an important role in body energy homeostasis (By similarity). Contributes to the biosynthesis of membrane phospholipids, cholesterol esters and triglycerides (By similarity).

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in fetal liver, lung and brain. Highly expressed in adult adipose tissue, and at lower levels in adult brain and lung.

Subunit Structure:

May self-associate and form homodimers.

Family&Domains:

The histidine box domains are involved in binding the catalytic metal ions.

Belongs to the fatty acid desaturase type 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Metabolism > Lipid metabolism > Biosynthesis of unsaturated fatty acids.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

References

1). Metabolomics reveals that CAF-derived lipids promote colorectal cancer peritoneal metastasis by enhancing membrane fluidity. International Journal of Biological Sciences (PubMed: 35342344) [IF=9.2]

Application: WB    Species: human    Sample: DLD1 and HCT116 cells

Figure 5. |Low expression of SCD is associated with poor prognosis in patients with CRC and PM-CRC. A, Schematic representation of fatty acid synthesis and function. B, Western blot showing SCD expression in DLD1 and HCT116 cells incubated with CAF-CM.

Application: IHC    Species: human    Sample: DLD1 and HCT116 cells

Figure 5. |Low expression of SCD is associated with poor prognosis in patients with CRC and PM-CRC. C, RT-qPCR was performed to measure the mRNA level of SCD in DLD1/HCT116 cells incubated with CAF-CM. D-E, Comparison of SCD protein expression by IHC (D).
2). AKAP8L enhances the stemness and chemoresistance of gastric cancer cells by stabilizing SCD1 mRNA. Cell Death & Disease (PubMed: 36522343) [IF=9.0]
3). Yogurt-derived Lactobacillus plantarum Q16 alleviated high-fat diet-induced non-alcoholic fatty liver disease in mice. Food Science and Human Wellness [IF=7.0]

Application: WB    Species: Mouse    Sample:

Fig. 5. Effects ofL. plantarum Q16 on key proteins involved in hepatic lipid metabolism in HFD-fed obese mice. Data are presented as mean ± SD (n = 6). Different lowercase alphabet letters were significantly different at the level of P < 0.05.
4). Peptides released from bovine α-lactalbumin by simulated digestion alleviated free fatty acids-induced lipid accumulation in HepG2 cells. Journal of Functional Foods [IF=5.6]
5). Patchouli alcohol ameliorates acute liver injury via inhibiting oxidative stress and gut-origin LPS leakage in rats. International Immunopharmacology (PubMed: 34182243) [IF=5.6]
6). Digeda-4 decoction and its disassembled prescriptions improve dyslipidemia and apoptosis by regulating AMPK/SIRT1 pathway on tyloxapol-induced nonalcoholic fatty liver disease in mice. Journal of Ethnopharmacology (PubMed: 37348794) [IF=5.4]
7). Sex hormone-binding globulin improves lipid metabolism and reduces inflammation in subcutaneous adipose tissue of metabolic syndrome-affected horses. Frontiers in molecular biosciences (PubMed: 38146533) [IF=5.0]
8). The enhancer RNA ADCY10P1 is associated with the progression of ovarian cancer. Journal of Ovarian Research (PubMed: 35568893) [IF=4.0]
9). Combined effects of ambient particulate matter exposure and a high-fat diet on oxidative stress and steatohepatitis in mice. PLoS One (PubMed: 30921449) [IF=3.7]

Application: WB    Species: mouse    Sample: liver

Fig 3. |Ambient PM exposure leads to hepatic steatosis by impairing hepatic lipid metabolism. (A) Oil Red O staining observation of liver (×200, scale bars = 100 μm). (B) H&E staining observation of liver (×200, scale bars = 50 μm). (C) The volume density of quantitation of hepatic steatosis (n = 5). (D) The genes expression involved in fatty acid β-oxidation in liver (n = 5). (E) The mRNA expression of genes involved in lipogenesis and FXR in liver (n = 5). (F) Bands of PPARα,PPARγ, ACOX1, FAS, SREBP-1c, SCD1.
10). Arctium lappa L. polysaccharide can regulate lipid metabolism in type 2 diabetic rats through the SREBP-1/SCD-1 axis. Carbohydrate Research (PubMed: 32535406) [IF=3.1]

Application: IHC    Species: rat    Sample: liver

Fig. 6.| Expression of SREBP-1 and SCD-1 in the liver by immunohistochemistry (**P < 0.01 vs. the NG group, ##P < 0.01 vs. the DM group).

Application: WB    Species: rat    Sample: liver

Fig. 7.| Detection of p-AMPK, AMPK, SREBP-1, and SCD-1 expression by Western blotting (**P < 0.01 vs. the DM group, n = 3).
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