Nicotinic Acetylcholine Receptor alpha 7 Antibody - #DF13247

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Product: Nicotinic Acetylcholine Receptor alpha 7 Antibody
Catalog: DF13247
Description: Rabbit polyclonal antibody to Nicotinic Acetylcholine Receptor alpha 7
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Chicken
Mol.Wt.: 56kDa, 48 kDa; 56kD(Calculated).
Uniprot: P36544
RRID: AB_2846266

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Chicken(100%)
Clonality:
Polyclonal
Specificity:
Nicotinic Acetylcholine Receptor alpha 7 Antibody detects endogenous levels of total Nicotinic Acetylcholine Receptor alpha 7.
RRID:
AB_2846266
Cite Format: Affinity Biosciences Cat# DF13247, RRID:AB_2846266.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

7nAChR; a7 nicotinic acetylcholine receptor; acetylcholine receptor, neuronal nicotinic, alpha-7 subunit; ACHA7_HUMAN; AChR; Acra7; alpha 7 neuronal nicotinic acetylcholine receptor; alpha-7 nicotinic cholinergic receptor subunit; alpha7; cholinergic receptor, neuronal nicotinic, alpha polypeptide 7; cholinergic receptor, nicotinic, alpha 7 (neuronal); cholinergic receptor, nicotinic, alpha 7; cholinergic receptor, nicotinic, alpha polypeptide 7; CHRNA7; CHRNA7-2; NACHRA7; neuronal acetylcholine receptor protein, alpha-7 chain; Neuronal acetylcholine receptor subunit alpha-7;

Immunogens

Immunogen:
Uniprot:

P36544(ACHA7_HUMAN) >>Visit HPA database.

Gene(ID):
CHRNA7(1139)
Sequence:
MRCSPGGVWLALAASLLHVSLQGEFQRKLYKELVKNYNPLERPVANDSQPLTVYFSLSLLQIMDVDEKNQVLTTNIWLQMSWTDHYLQWNVSEYPGVKTVRFPDGQIWKPDILLYNSADERFDATFHTNVLVNSSGHCQYLPPGIFKSSCYIDVRWFPFDVQHCKLKFGSWSYGGWSLDLQMQEADISGYIPNGEWDLVGIPGKRSERFYECCKEPYPDVTFTVTMRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLQYHHHDPDGGKMPKWTRVILLNWCAWFLRMKRPGEDKVRPACQHKQRRCSLASVEMSAVAPPPASNGNLLYIGFRGLDGVHCVPTPDSGVVCGRMACSPTHDEHLLHGGQPPEGDPDLAKILEEVRYIANRFRCQDESEAVCSEWKFAACVVDRLCLMAFSVFTIICTIGILMSAPNFVEAVSKDFA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Chicken
100
Xenopus
75
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P36544 As Substrate

Site PTM Type Enzyme
Y30 Phosphorylation
T83 Phosphorylation
Y86 Phosphorylation
Y217 Phosphorylation
T221 Phosphorylation
T225 Phosphorylation
S365 Phosphorylation
Y386 Phosphorylation P12931 (SRC)
Y442 Phosphorylation P12931 (SRC)

Research Backgrounds

Function:

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.

Subcellular Location:

Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homopentamer (By similarity). Interacts with RIC3; which is required for proper folding and assembly. Interacts with LYPD6. Interacts with the alpha-conotoxin RgIA (By similarity). Interacts with alpha-conotoxins ImI and ImII.

Family&Domains:

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Organismal Systems > Nervous system > Cholinergic synapse.

References

1). Gelatin nanoparticles loaded with 3-alkylpyridinium salt APS7, an analog of marine toxin, are a promising support in human lung cancer therapy. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38906020) [IF=7.5]
2). Prenatal Hypoxia Triggers a Glucocorticoid-Associated Depressive-like Phenotype in Adult Rats, Accompanied by Reduced Anxiety in Response to Stress. International journal of molecular sciences, 2024 (PubMed: 38892090) [IF=5.6]
3). α7nAChR-mediated astrocytic activation: a novel mechanism of Xiongzhi Dilong decoction in ameliorating chronic migraine. Journal of ethnopharmacology, 2024 (PubMed: 38971346) [IF=5.4]
4). α-Mangostin Treats Early-Stage Adjuvant-Induced Arthritis of Rat by Regulating the CAP-SIRT1 Pathway in Macrophages. Drug Design, Development and Therapy, 2022 (PubMed: 35250263) [IF=4.8]

Application: WB    Species: Rat    Sample: RAW264.7 Cells

Figure 4 Effect of MG activated CAP-SIRT1 pathway on NF-κB-mediated inflammatory response in rat macrophages. (A) Expression of α7nAChR, SIRT1, ac-p65, p-p65 and p65 in macrophages investigated by immunoblotting; (B–E) quantitative result of (A). #P<0.05, ##P<0.01, ###P<0.001 compared with normal, *P<0.05, **P<0.01, ***P<0.001 compared with AIA rats, &P<0.05 compared with MG (early).
5). The prenatal hypoxic pathology associated with maternal stress predisposes to dysregulated expression of the chrna7 gene and the subsequent development of nicotine addiction in adult offspring. Neuroendocrinology, 2024 (PubMed: 38198758) [IF=4.1]

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